Treatment of clostridium difficile infection

ABSTRACT

Provided herein are compositions and methods for the treatment or prevention of pathogenic infections.

RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No.15/993,037, filed May 30, 2018, which is a continuation of U.S.application Ser. No. 15/630,088, filed Jun. 22, 2017, which is acontinuation of international application number PCT/US2017/037498,filed Jun. 14, 2017, which claims the benefit under 35 U.S.C. § 119(e)of U.S. provisional application No. 62/349,914, filed Jun. 14, 2016,each of which is incorporated by reference herein in its entirety.

FIELD OF INVENTION

The disclosure relates to compositions of purified bacterial strains,and methods for treating pathogenic infections, such as Clostridiumdifficile infections, by administering the compositions to a subjecthaving a pathogenic infection.

BACKGROUND OF THE INVENTION

The collection of bacterial, viral, and fungal commensal microorganismsthat reside within and on the human body are collectively known as thehuman microbiome. The bacterial subset of the human microbiome plays animportant role in host nutrient acquisition, development, immunologicalhomeostasis, neurological health, and protection against pathogens(LeBlanc et al. Curr. Opin. Biotechnol. (2013) 24(2): 160-168; Hooper etal. Science (2012) 336(6086): 1268-1273; Hughes et al. Am. J.Gastroenterol. (2013) 108(7): 1066-1074). As the largest reservoir ofmammalian commensals, bacteria residing in the gastrointestinal (GI)tract influence nearly all of these aspects of human biology (Blaser J.Clin. Invest. (2014) 124(10): 4162-4165). Consequently, perturbation ofthe normal bacterial populations within the GI niche, a state known asdysbiosis, can predispose humans to a variety of diseases.

Clostridium difficile infection (CDI) arises after intestinalcolonization by the anaerobic spore-forming Gram-positive pathogenClostridium difficile. Upon colonization of the GI tract, C. difficileproduces toxins which causes diarrhea and may ultimately lead to death.This illness is the most common identifiable cause of nosocomialdiarrhea and is thought to arise as a direct result of dysbiosis (CalfeeGeriatrics (2008) 63: 10-21; Shannon-Lowe et al BMJ (2010) 340: c1296).Not surprisingly, usage of nearly all classes of antibiotics has beenassociated with CDI, presumably by inducing dysbiosis in the GI tractand thereby enabling C. difficile outgrowth. The Center for DiseaseControl currently classifies CDI as a public health threat requiringimmediate and aggressive action because of its natural resistance tomany drugs and the emergence of a fluoroquinolone-resistant strain thatis now prevalent throughout North America and Europe. C. difficile wasresponsible for almost half a million infections and was associated withapproximately 29,000 deaths in 2011 (Lessa et al. NEJM 2015, 372:825-834).

The antibiotics metronidazole, vancomycin, and fidaxomicin are thecurrent therapeutic options for treatment of CDI. However, metronidazoleis inadequate because of decreased response rates and neithermetronidazole nor vancomycin prevent disease recurrence, with up to 30%of patients initially responding experiencing a clinical recurrenceafter antibiotic cessation (Miller Expert Opin. Pharmacother. (2010) 11:1569-1578). Fidaxomicin has been shown to be superior to vancomycin inpreventing recurrent CDI (Mullane Ther. Adv. Chronic Dis. (2014) 5(2):69-84). Because of its narrow spectrum of activity, fidaxomicin isthought to enable normal microbiome repopulation of the gut followingdysbiosis and CDI, thereby lowering the likelihood of recurrent disease(Tannock et al. Microbiology (2010) 156 (Pt 11): 3354-3359; Louie et al.Clin. Infect. Dis. (2012) 55 Suppl. 2: S132-142). Nonetheless, 14% offidaxomicin-treated patients experience CDI relapse and mutationsconferring reduced sensitivity have already been reported (Eyre et al.J. Infect. Dis. (2014) 209(9): 1446-1451).

Because the risk of recurrent CDI is heightened by antibiotic use and C.difficile spores are inherently recalcitrant to the availablechemotherapeutic arsenal, alternative therapeutic modalities are beingpursued for the treatment of CDI. Fecal microbiota transplantation (FMT)is one such modality that has shown efficacy against CDI (Khoruts et al.Immunol. Lett. (2014) 162(2): 77-81; van Nood et al. N. Engl. J. Med.(2013) 368(5): 407-415). To date, results of FMT studies for thetreatment of CDI, have reported cure rates up to 90% in three randomizedcontrolled studies (Cammarota et al. Alimen. Pharmacol. Therap. (2015)41(9): 835-843; Kassam et al. Am. J. Gastroenterol. (2013) 108(4):500-508; van Nood et al. N. Engl. J. Med. (2013) 368(5): 407-415;Youngster et al. Infec. Dis. Soc. Am. (2014) 58(11): 1515-1522).

Despite the success of FMT, this therapeutic approach is not withoutrisks and logistical concerns. Selection of FMT donors is critical andchallenging. When FMT donor recruitment is performed with stringentscreening and standardization protocols, most prospective donors failthis process. Only 6-10% of prospective FMT donors qualify, with themajority of failures arising from asymptomatic carriage of GI pathogens(Paramsothy et al. Inflamm. Bowel Dis. (2015) 21(7): 1600-1606; Borodyet al. Curr. Opin. Gastroenterol. (2014) 30(10): 97-105; Burns et al.Gastroenterology (2015) 148: S96-S97; Surawicz Ann. Intern. Med. (2015)162(9): 662-663). Furthermore, variation between donors may lead tovariation in FMT efficacy. In addition, the risk of transmission of evennon-infectious illnesses may be heightened by FMT. Indeed, significantweight gain has been reported in a patient who received an FMT from anoverweight stool donor (Alang et al. Open Forum Infect. Dis. (Winter2015) 2(1)).

SUMMARY OF THE INVENTION

Provided herein are compositions and methods for the treatment orprevention of pathogenic infections including C. difficile.

In one aspect, the disclosure provides compositions comprising two ormore purified bacterial strains of species selected from the groupconsisting of: Clostridium hathewayi, Blautia hansenii, Blautiaproducta, Blautia producta ATCC 27340, Clostridium bacterium UC5.1-1D4,Blautia coccoides, Eubacterium contortum, Eubacterium fissicatena,Sellimonas intestinalis, Dracourtella massiliensis, Dracourtellamassilinesis GD1, Ruminococcus torques, Anaerostipes caccae, Clostridiumscindens, Marvinbryanta formatexigens, Eisenbergiella tayi,Flavinofractor plautii, Clostridium orbiscindens 1_3_50AFAA,Lachnospiraceae bacterium 7_1_58FAA, Subdoligranulum, Anaerotruncuscolihominis, Anaerotruncus colihominis DSM 17241, Clostridium symbiosum,Clostridium symbiosum WAL-14163, Clostridium bolteae, Clostridiumbolteae 90A9, Dorea longicatena, Dorea longicatena CAG:42, Clostridiuminnocuum, Erysipelotrichaceae_bacterium_21-3, Blautia wexlerae,Clostridium disporicum, Erysipelatoclostridium ramosum,Pseudoflavinofractor capillosus, Turicibacter sanguinis, Lactobacillusmucosae, Ruminococcus obeum, Megasphaera elsdenii, Acidaminococcusfermentans, Acidaminococcus intestine, Ruminococcus faecis, Bacteroidescellulosilyticus, Anaerostipes hadrus, Eubacterium rectale, Ruminococcuschampanellnsis, Ruminococcus albus, Bifidobacterium bifidum, Blautialuti, Roseburia faecis, Fusicatenibacter saccharivorans, Roseburiafaecis, Blautia faecis, Dorea formicigenerans and Bacteroides ovatus.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Clostridium hathewayi, Blautia hansenii,Blautia producta, Blautia producta ATCC 27340, Clostridium bacteriumUC5.1-1D4, Blautia coccoides, Eubacterium contortum, Eubacteriumfissicatena, Sellimonas intestinalis, Dracourtella massiliensis,Dracourtella massilinesis GD1, Ruminococcus torques, Anaerostipescaccae, Clostridium scindens, Marvinbryanta formatexigens,Eisenbergiella tayi, Flavinofractor plautii, Clostridium orbiscindens1_3_50AFAA, Lachnospiraceae bacterium 7_1_58FAA, Subdoligranulum,Anaerotruncus colihominis, Anaerotruncus colihominis DSM 17241,Clostridium symbiosum, Clostridium symbiosum WAL-14163, Clostridiumbolteae, Clostridium bolteae 90A9, Dorea longicatena, Dorea longicatenaCAG:42, Clostridium innocuum, Erysipelotrichaceae_bacterium_21-3,Blautia wexlerae, Turicibacter sanguinis, Lactobacillus mucosae, andBacteroides ovatus.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Clostridium hathewayi, Blautia hansenii,Blautia producta, Blautia coccoides, Eubacterium contortum, Eubacteriumfissicatena, Anaerostipes caccae, Clostridium scindens, Marvinbryantaformatexigens, and Eisenbergiella tayi.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Flavinofractor plautii, Clostridiumorbiscindens 1_3_50AFAA, Lachnospiraceae bacterium 7_1_58FAA,Subdoligranulum, Anaerotruncus colihominis, Anaerotruncus colihominisDSM 17241, Eubacterium fissicatena, Sellimonas intestinalis,Dracourtella massiliensis, Dracourtella massilinesis GD1, Ruminococcustorques, Clostridium symbiosum, Clostridium symbiosum WAL-14163,Clostridium bolteae, Clostridium bolteae 90A9, Dorea longicatena, Dorealongicatena CAG:42, Blautia producta, Blautia producta ATCC 27340,Clostridium bacterium UC5.1-1D4, Clostridium innocuum, andErysipelotrichaceae_bacterium_21-3.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Clostridium orbiscindens 1_3_50AFAA,Anaerotruncus colihominis DSM 17241, Dracourtella massilinesis GD1,Clostridium symbiosum WAL-14163, Clostridium bolteae 90A9, Dorealongicatena CAG:42, Clostridium bacterium UC5.1-1D4, andErysipelotrichaceae_bacterium_21-3.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Clostridium orbiscindens 1_3_50AFAA,Anaerotruncus colihominis DSM 17241, Sellimonas intestinalis,Clostridium symbiosum WAL-14163, Clostridium bolteae 90A9, Dorealongicatena CAG:42, Clostridium bacterium UC5.1-1D4, andErysipelotrichaceae_bacterium_21_3.

In some embodiments of the compositions provided herein, the compositioncomprises purified bacterial strains Clostridium orbiscindens1_3_50AFAA, Anaerotruncus colihominis DSM 17241, Dracourtellamassilinesis GD1, Clostridium symbiosum WAL-14163, Clostridium bolteae90A9, Dorea longicatena CAG:42, Clostridium bacterium UC5.1-1D4, andErysipelotrichaceae_bacterium_21-3.

In some embodiments of the compositions provided herein, the compositioncomprises purified bacterial strains Clostridium orbiscindens1_3_50AFAA, Anaerotruncus colihominis DSM 17241, Sellimonas intestinalisGD1, Clostridium symbiosum WAL-14163, Clostridium bolteae 90A9, Dorealongicatena CAG:42, Clostridium bacterium UC5.1-1D4, andErysipelotrichaceae_bacterium_21_3.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Flavinofractor plautii, Anaerotruncuscolihominis, Dracourtella massiliensis, Clostridium symbiosum,Clostridium bolteae, Dorea longicatena, Blautia producta, andClostridium innocuum.

In some embodiments of the compositions provided herein, the compositioncomprises purified bacterial strains Flavinofractor plautii,Anaerotruncus colihominis, Dracourtella massiliensis, Clostridiumsymbiosum, Clostridium bolteae, Dorea longicatena, Blautia producta, andClostridium innocuum.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Flavinofractor plautii, Anaerotruncuscolihominis, Eubacterium fissicatena, Clostridium symbiosum, Clostridiumbolteae, Dorea longicatena, Blautia producta, and Clostridium innocuum.

In some embodiments of the compositions provided herein, the compositioncomprises purified bacterial strains Flavinofractor plautii,Anaerotruncus colihominis, Eubacterium fissicatena, Clostridiumsymbiosum, Clostridium bolteae, Dorea longicatena, Blautia producta, andClostridium innocuum.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Flavinofractor plautii, Lachnospiraceaebacterium 7_1_58FAA, Subdoligranulum, Anaerotruncus colihominis,Eubacterium fissicatena, Ruminococcus torques, Clostridium symbiosum,Clostridium bolteae, Dorea longicatena, Blautia producta, Clostridiuminnocuum, Erysipelotrichaceae_bacterium_21-3, and Bacteroides ovatus.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Clostridium orbiscindens 1_3_50 AFAA,Anaerotruncus colihominis DSM 17241, Dracourtella massiliensis GD1,Clostridium symbiosum WAL-14163, Clostridium bolteae 90A9, Dorealongicatena CAG:42, Clostridium bacterium UC5.1-1D4,Dysipelotrichaceae_bacterium_21-3, and Bacteroides ovatus.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Clostridium orbiscindens 1_3_50AFAA,Anaerotruncus colihominis DSM 17241, Sellimonas intestinalis,Clostridium symbiosum WAL-14163, Clostridium bolteae 90A9, Dorealongicatena CAG:42, Clostridium bacterium UC5.1-1D4,Erysipelotrichaceae_bacterium_21-3, and Bacteroides ovatus.

In some embodiments of the compositions provided herein, the compositiondoes not include a bacterial strain of the species Flavinofractorplautii, Subdoligranulum, or Lachnospiraceae bacterium 7_1_58FAA. Insome embodiments of the compositions provided herein, the compositiondoes not include a bacterial strain of the species Bacteroides ovatus.The composition of any one of claims 4-12, wherein the composition doesnot include a bacterial strain of the species Flavinofractor plautii,Subdoligranulum, Clostridium orbiscindens 1_3_50AFAA, or Lachnospiraceaebacterium 7¬1_58 FAA.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Clostridium scindens, Clostridiumhathewayi, Blautia hansenii, Blautia wexlerae, Blautia producta, Blautiacoccoides, Dorea longicatena, Clostridium innocuum,Erysipelotrichaceae_bacterium_21-3, Flavinofractor plautii,Lachnospiraceae bacterium 7-1_58FAA, Subdoligranulum, Anaerotruncuscolihominis, and Clostridium symbiosum. In some embodiments of thecompositions provided herein, the composition does not include abacterial strain of the species Flavinofractor plautii, Subdoligranulum,or Lachnospiraceae bacterium 7_1_58FAA.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Clostridium scindens, Clostridiumhathewayi, Blautia hansenii, Blautia wexlerae, Anaerotruncuscolihominis, Dorea longicatena, Clostridium innocuum,Erysipelotrichaceae_bacterium_21-3, Flavinofractor plautii,Lachnospiraceae bacterium 7-1_58FAA, Subdoligranulum, Turicibactersanguinis, and Lactobacillus mucosae. In some embodiments of thecompositions provided herein, the composition does not include abacterial strain of the species Flavinofractor plautii, Subdoligranulumor Lachnospiraceae bacterium 7_1_58FAA.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Dorea longicatena, Ruminococcus obeum,Megasphaera elsdenii, Acidaminococcus fermentans, Acidaminococcusintestine, Ruminococcus faecis, Bacteroides cellulosilyticus,Anaerostipes hadrus, Flavinofractor plautii, Eubacterium rectale,Ruminococcus champanellensis, Ruminococcus albus, Bifidobacteriumbifidum, Ruminococcus faecis, Blautia luti, Roseburia faecis,Fusicatenibacter saccharivorans, Blautia faecis, Dorea formicigenerans,and Blautia hansenii.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains of species selectedfrom the group consisting of: Acidaminococcus fermentans,Acidaminococcus intestine, Anaerostipes hadrus, Blautia faecis, Blautiahansenii, Dorea formicigenerans, Dorea longicatena, Eubacterium rectale,Flavinofractor plautii, Fusicatenibacter saccharivorans, Megasphaeraelsdenii, Roseburia faecis, Ruminococcus champanellensis, Ruminococcusalbus, Ruminococcus faecis, and Ruminococcus obeum.

In one aspect the disclosure provides compositions comprising two ormore purified bacterial strains, wherein the two or more purifiedbacterial strains comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NOs:1-83 and 124-159.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs:1-23, SEQ ID NO:83, SEQ ID NOs: 124-159.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ IDNO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ IDNO:23.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NOs:124-159. In some embodiments of the compositions provided herein, thecomposition comprises two or more purified bacterial strains, whereinthe two or more purified bacterial strains comprise 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ IDNO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21. In someembodiments of the compositions provided herein, the compositioncomprises purified bacterial strains that comprise 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ IDNO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21. In someembodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs: 124-159. In some embodiments of thecompositions provided herein, the composition comprises two or morepurified bacterial strains, wherein the two or more purified bacterialstrains comprise 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ ID NO:124, SEQ ID NO: 129, SEQ ID NO: 132, SEQ ID NO: 137, SEQ ID NO: 141, SEQID NO: 146, SEQ ID NO: 152, and SEQ ID NO: 157. In some embodiments ofthe compositions provided herein, the composition comprises purifiedbacterial strains that comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO: 124, SEQ ID NO: 129, SEQ ID NO: 132, SEQ ID NO: 137, SEQID NO: 141, SEQ ID NO: 146, SEQ ID NO: 152, and SEQ ID NO: 157.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20 and SEQ ID NO:21, and wherein the compositiondoes not include a bacterial strain comprising a 16S rDNA sequencehaving at least 97% homology with a nucleic acid sequence of SEQ IDNO:10. In some embodiments of the compositions provided herein, thecomposition comprises two or more purified bacterial strains, whereinthe two or more purified bacterial strains comprise 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NOs:124-156, and wherein the compositiondoes not include a bacterial strain comprising a 16S rDNA sequencehaving at least 97% homology with a nucleic acid sequence selected fromthe group consisting of SEQ ID NOs:157-159.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21 and SEQ ID NO:22. In someembodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequence selected from the groupconsisting of SEQ ID NO: 124-145, SEQ ID NO: 152-159, SEQ ID NO: 18, andSEQ ID NO: 22.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:21 and SEQ ID NO:22, and wherein the compositiondoes not include a bacterial strain comprising a 16S rDNA sequencehaving at least 97% homology with a nucleic acid sequence of SEQ IDNO:10. In some embodiments of the compositions provided herein, thecomposition comprises two or more purified bacterial strains, whereinthe two or more purified bacterial strains comprise 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO: 124-145 and SEQ ID NO: 152-156, andwherein the composition does not include a bacterial strain comprising a16S rDNA sequence having at least 97% homology with a nucleic acidsequence selected from the group consisting of SEQ ID NOs:157-159.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10 and SEQ ID NOs:14-22. In some embodiments ofthe compositions provided herein, the composition comprises two or morepurified bacterial strains, wherein the two or more purified bacterialstrains comprise 16S rDNA sequences having at least 97% homology withnucleic acid sequence selected from the group consisting of SEQ ID NOs:124-159, SEQ ID NO: 18, and SEQ ID NO: 22.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs:14-22 and wherein the composition does notinclude a bacterial strain comprising a 16S rDNA sequence having atleast 97% homology with a nucleic acid sequence of SEQ ID NO:10. In someembodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs: 129-156, SEQ ID NO: 18, SEQ ID NO: 22, andwherein the composition does not include a bacterial strain comprising a16S rDNA sequence having at least 97% homology with a nucleic acidsequence selected from the group consisting of SEQ ID NOs:157-159.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 and SEQ ID NO:83.In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID Nos: 124-159 and SEQ ID NO: 83.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 and SEQ ID NO:83, and whereinthe composition does not include a bacterial strain comprising a 16SrDNA sequence having at least 97% homology with a nucleic acid sequenceof SEQ ID NO:10. In some embodiments of the compositions providedherein, the composition comprises two or more purified bacterialstrains, wherein the two or more purified bacterial strains comprise 16SrDNA sequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NOs: 124-156 and SEQ ID NO:83, and wherein composition does not include a bacterial straincomprising a 16S rDNA sequence having at least 97% homology with anucleic acid sequence selected from the group consisting of SEQ IDNOs:157-159.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22 and SEQ ID NO:83.In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs: 124-159, SEQ ID NO: 22, and SEQ ID NO: 83,

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22 and SEQ ID NO:83, and whereinthe composition does not include a bacterial strain comprising a 16SrDNA sequence having at least 97% homology with a nucleic acid sequenceof SEQ ID NO:10. In some embodiments of the compositions providedherein, the composition comprises two or more purified bacterialstrains, wherein the two or more purified bacterial strains comprise 16SrDNA sequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NOs: 124-145, SEQ ID NOs:152-156, SEQ ID NO: 22, and SEQ ID NO: 83, wherein composition does notinclude a bacterial strain comprising a 16S rDNA sequence having atleast 97% homology with a nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:157-159.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NOs:14-22, and SEQ ID NO:83. In someembodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs: 124-159, SEQ ID NO: 18, SEQ ID NO:22, and SEQID NO: 83.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs:14-22 and SEQ ID NO:83, and wherein thecomposition does not include a bacterial strain comprising a 16S rDNAsequence having at least 97% homology with a nucleic acid sequence ofSEQ ID NO:10. In some embodiments of the compositions provided herein,the composition comprises two or more purified bacterial strains,wherein the two or more purified bacterial strains comprise 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO: 124-15, SEQ ID NO: 18,SEQ ID NO:22, and SEQ ID NO: 83, wherein composition does not include abacterial strain comprising a 16S rDNA sequence having at least 97%homology with a nucleic acid sequence selected from the group consistingof SEQ ID NOs:157-159.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ IDNO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, and SEQID NO:21.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:5, SEQ IDNO:6, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:18, and SEQ IDNO:21.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs:24-79.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs:24-27, SEQ ID NO:32, SEQ ID NO:34, SEQ IDNO:35, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:43, SEQ IDNO:44, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:51, SEQ ID NO:55, SEQ IDNO:56, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:68, SEQ IDNO:70, SEQ ID NO:72, SEQ ID NO:76 and SEQ ID NO:77.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:21, and SEQ IDNOs:80-82.

In one aspect the disclosure provides compositions comprising two ormore purified bacterial strains, wherein the two or more purifiedbacterial strains comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NOs:84-123.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:87, SEQ ID NO:88, SEQ ID NO:89, SEQ ID NO:90,SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:94, SEQ ID NO:95, SEQ ID NO:97,SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103,SEQ ID NO:105, SEQ ID NO:106, SEQ ID NO:108, SEQ ID NO:109, SEQ IDNO:110, SEQ ID NO:121, and SEQ ID NO:122.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:87, SEQ ID NO:88, SEQ ID NO:89, SEQ ID NO:99,SEQ ID NO:103, SEQ ID NO:105, SEQ ID NO:106, SEQ ID NO:108, SEQ IDNO:109, and SEQ ID NO:121.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98,SEQ ID NO:102, SEQ ID NO:106, SEQ ID NO:110, and SEQ ID NO:122.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:102,SEQ ID NO:106, SEQ ID NO:110, and SEQ ID NO:122, and wherein thecomposition does not include a bacterial strain comprising a 16S rDNAsequence having at least 97% homology with a nucleic acid sequence ofSEQ ID NO:93.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98,SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:106, SEQ ID NO:110, and SEQ IDNO:122.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:101,SEQ ID NO:102, SEQ ID NO:106, SEQ ID NO:110, and SEQ ID NO:122, andwherein the composition does not include a bacterial strain comprising a16S rDNA sequence having at least 97% homology with a nucleic acidsequence of SEQ ID NO:93.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:87, SEQ ID NO:93, SEQ ID NO:94, SEQ ID NO:95,SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:103, SEQ ID NO:105,SEQ ID NO:106, and SEQ ID NO:122.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:87, SEQ ID NO:90, SEQ ID NO:91, SEQ ID NO:93,SEQ ID NO:94, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99,and SEQ ID NO:105.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:92, SEQ ID NO:93,SEQ ID NO:96, SEQ ID NO:97, SEQ ID NO:99, SEQ ID NO:100, SEQ ID NO:104,SEQ ID NO:107, SEQ ID NO:111, SEQ ID NO:112, SEQ ID NO:113, SEQ IDNO:114, SEQ ID NO:115, SEQ ID NO:116, SEQ ID NO:117, SEQ ID NO:118, SEQID NO:119, and SEQ ID NO:120.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:92, SEQ ID NO:93,SEQ ID NO:96, SEQ ID NO:97, SEQ ID NO:99, SEQ ID NO:104, SEQ ID NO:107,SEQ ID NO:111, SEQ ID NO:112, SEQ ID NO:113, SEQ ID NO:114, SEQ IDNO:115, SEQ ID NO:116, SEQ ID NO:117, and SEQ ID NO:119.

In some embodiments of the compositions provided herein, the compositioncomprises two or more purified bacterial strains, wherein the two ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:86, SEQ ID NO:95, SEQ ID NO:98, SEQ ID NO:110,SEQ ID NO:122, and SEQ ID NO:123.

In some embodiments of the compositions provided herein, the compositioncomprises at least one bacterial strain from Clostridium cluster XIVaand at least one bacterial strain from Clostridium cluster XVII. In someembodiments of the compositions provided herein, the compositioncomprises at least one bacterial strain from Clostridium cluster IV andat least one bacterial strain from Clostridium cluster XVII. In someembodiments of the compositions provided herein, the compositioncomprises at least one bacterial strain from Clostridium cluster XIVa,at least one strain from Clostridium cluster IV and at least onebacterial strain from Clostridium cluster XVII.

In some embodiments of the compositions provided herein, the compositioncomprises at least one Bacteroides strain. In some embodiments of thecompositions provided herein, the composition does not includeClostridium scindens.

In some embodiments of the compositions provided herein, the compositioncomprises at least 3, at least 4, at least 5, at least 6, at least 7, atleast 8, at least 9, at least 10, at least 11, at least 12, at least 13,at least 14, at least 15, at least 16, at least 17, at least 18, atleast 19, or at least 20 purified bacterial strains.

In some embodiments of the compositions provided herein, one or more ofthe bacterial strains are spore formers. In some embodiments of thecompositions provided herein, one or more of the bacterial strains arein spore form. In some embodiments of the compositions provided herein,each of the bacterial strains is in spore form.

In some embodiments of the compositions provided herein, one or more ofthe bacterial strains is in vegetative form. In some embodiments of thecompositions provided herein, each of the bacterial strains is invegetative form.

In some embodiments of the compositions provided herein, the compositioncomprises only obligate anaerobic bacterial strains. In some embodimentsof the compositions provided herein, the composition comprises bacterialstrains that originate from more than one human donor.

In some embodiments of the compositions provided herein, one or more ofthe bacterial strains are baiCD−. In some embodiments of thecompositions provided herein, each of the bacterial strains is baiCD−.In some embodiments of the compositions provided herein, the compositiondoes not mediate bile acid 7-alpha-dehydroxylation. In some embodimentsof the compositions provided herein, the composition inhibits C.difficile toxin production. In some embodiments of the compositionsprovided herein, the composition inhibits C. difficile replicationand/or survival.

In some embodiments of the compositions provided herein, the bacterialstrains are lyophilized.

In some embodiments of the compositions provided herein, the compositioninduces the proliferation and/or accumulation of regulatory T cells(Tregs).

In one aspect, the disclosure provides compositions comprising two ormore purified bacterial strains, wherein the composition comprises atleast one bacterial strain from Clostridium cluster XIVa and at leastone bacterial strain from Clostridium cluster XVII. In one aspect, thedisclosure provides compositions comprising two or more purifiedbacterial strains, wherein the composition comprises at least onebacterial strain from Clostridium cluster IV and at least one bacterialstrain from Clostridium cluster XVII. In one aspect, the disclosureprovides compositions comprising two or more purified bacterial strains,wherein the composition comprises at least one bacterial strain fromClostridium cluster IV, at least one bacterial strain from Clostridiumcluster XIVa and at least one bacterial strain from Clostridium clusterXVII.

In some embodiments of the compositions provided herein, the compositioncomprises at least one Bacteroides strain. In some embodiments of thecompositions provided herein, the composition does not includeClostridium scindens.

In some embodiments of the compositions provided herein, the compositioncomprises at least 3, at least 4, at least 5, at least 6, at least 7, atleast 8, at least 9, at least 10, at least 11, at least 12, at least 13,at least 14, at least 15, at least 16, at least 17, at least 18, atleast 19, or at least 20 purified bacterial strains.

In some embodiments of the compositions provided herein, one or more ofthe bacterial strains are spore formers. In some embodiments of thecompositions provided herein, one or more of the bacterial strains arein spore form. In some embodiments of the compositions provided herein,each of the bacterial strains is in spore form.

In some embodiments of the compositions provided herein, one or more ofthe bacterial strains is in vegetative form. In some embodiments of thecompositions provided herein, each of the bacterial strains is invegetative form.

In some embodiments of the compositions provided herein, the compositioncomprises only obligate anaerobic bacterial strains.

In some embodiments of the compositions provided herein, the compositioncomprises bacterial strains that originate from more than one humandonor.

In some embodiments of the compositions provided herein, one or more ofthe bacterial strains are baiCD−. In some embodiments of thecompositions provided herein, each of the bacterial strains is baiCD−.In some embodiments of the compositions provided herein, the compositiondoes not mediate bile acid 7-alpha-dehydroxylation. In some embodimentsof the compositions provided herein, the composition inhibits C.difficile toxin production. In some embodiments of the compositionsprovided herein, the composition inhibits C. difficile replicationand/or survival.

In some embodiments of the compositions provided herein, the bacterialstrains are lyophilized.

In some embodiments of the compositions provided herein, the compositioninduces the proliferation and/or accumulation of regulatory T cells(Tregs).

In one aspect, the disclosure provides a pharmaceutical compositioncomprising any of the compositions provided herein further comprising apharmaceutically acceptable excipient. In some embodiments of thepharmaceutical compositions provided herein, the pharmaceuticalcomposition is formulated for oral delivery. In some embodiments of thepharmaceutical compositions provided herein, the pharmaceuticalcomposition is formulated for rectal delivery. In some embodiments ofthe pharmaceutical compositions provided herein, the pharmaceuticalcomposition is formulated for delivery to the intestine. In someembodiments of the pharmaceutical compositions provided herein, thepharmaceutical composition is formulated for delivery to the colon. Inone aspect, the disclosure provides a food product comprising any of thecompositions provided herein further comprising a nutrient.

In one aspect, the disclosure provides a method of treating a pathogenicinfection in a subject, comprising administering to the subject atherapeutically effective amount of any of the compositions or foodproducts provided herein to treat the pathogenic infection. In someembodiments of the methods provided herein, the pathogenic infection isC. difficile, Vancomycin Resistant Enterococci (VRE), CarbapenemResistant Enterobacteriaceae (CRE), Neisseria gonorrheae, MultidrugResistant Acinetobacter, Campylobacter, Extended spectrum beta-lactamese(ESBL) producing Enterobacteriaceae, Multidrug Resistant Pseudomonasaeruginosa, Salmonella, Drug resistant non-typhoid Salmonella, Drugresistant Salmonella Typhi, Drug resistant Shigella, MethicillinResistant Staphylococcus aureus, Drug resistant Streptococcuspneumoniae, Drug resistant Tuberculosis, Vancomycin resistantStaphylococcus aureus, Erythromycin Resistant Group A Streptococcus,Clindamycin resistant Group B Streptococcus, and combinations thereof.In some embodiments of the methods provided herein, the pathogenicinfection is C. difficile. In some embodiments of the methods providedherein, the pathogenic infection is Vancomycin-Resistant Enterococci.

In some embodiments of the methods provided herein, the subject ishuman. In some embodiments of the methods provided herein, the subjectis an asymptotic carrier.

In some embodiments of the methods provided herein, the subject isadministered a dose of an antibiotic prior to administration of thecomposition. In some embodiments of the methods provided herein, thesubject is administered more than one dose of the antibiotic prior toadministration of the composition. In some embodiments of the methodsprovided herein, the subject has not been administered an antibioticprior to administration of the composition.

In some embodiments of the methods provided herein, the composition isadministered to the subject by oral administration. In some embodimentsof the methods provided herein, the composition is administered to thesubject by rectal administration.

In some embodiments of the methods provided herein, the administeringresults in proliferation and/or accumulation of regulatory T cells(Tregs).

Each of the limitations of the invention can encompass variousembodiments of the invention. It is, therefore, anticipated that each ofthe limitations of the invention involving any one element orcombinations of elements can be included in each aspect of theinvention. This invention is not limited in its application to thedetails of construction and the arrangement of components set forth inthe following description or illustrated in the drawings. The inventionis capable of other embodiments and of being practiced or of beingcarried out in various ways.

BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying drawings are not intended to be drawn to scale. Thefigures are illustrative only and are not required for enablement of thedisclosure. For purposes of clarity, not every component may be labeledin every drawing. In the drawings:

FIG. 1 shows the strains of Compositions A-D. Each entry includes theSEQ ID NO of the 16S rDNA sequence of the strain, a strain identifier,and the species with the closest known homology (can be more than onespecies). The bracketed roman numeral indicates the Clostridium clusterclassification of each strain based on the closest species homology.Strains that are not classified in Cluster XIVa are highlighted in bold.The two non-clostridial strains (SEQ ID NO:2, closest known speciesTuricibacter sanguinis, and SEQ ID NO:6, closest known speciesLactobacillus mucosae) do not belong to the Clostridium genus.

FIG. 2 shows various Clostridium difficile infection models. Timelinesindicate antibiotic type, duration of treatment, as well as exposure toC. difficile spores. The top panel shows an antibiotic cocktailtreatment model in which the antibiotic cocktail is provided in thedrinking water from day −10 to day −3 followed by intraperitonealclindamycin on day −1. The middle panel shows a clindamycin IP injectionmodel, in which clindamycin is administered by intraperitoneal injectionon day −1. The bottom panel shows the cefoperazone treatment model, inwhich cefoperazone is provided in the drinking water from day −12 to day−2, followed by administration of a live biotherapeutic product (LBP) onday −1.

FIG. 3 shows the experimental conditions described in Example 1. Thegroups of mice were divided based on the antibiotic regimen receivedprior to administration of the indicated amount of C. difficile spores.“Abx” refers to treatment with any of the antibiotic regimens.

FIGS. 4A-4L show data obtained in Example 1. FIGS. 4A-4D show survivalof mice that received no treatment (FIG. 4A), antibiotic cocktail (FIG.4B), clindamycin (FIG. 4C), or cefoperazone (FIG. 4D) prior to C.difficile infection. FIGS. 4E-4H show body weight of mice that receivedno treatment (FIG. 4E), antibiotic cocktail (FIG. 4F), clindamycin (FIG.4G), or cefoperazone (FIG. 4H) prior to C. difficile infection. FIGS.4I-4L show C. difficile burden (CFU) per gram of feces from mice thatreceived no treatment (FIG. 4I), antibiotic cocktail (FIG. 4J),clindamycin (FIG. 4K), or cefoperazone (FIG. 4L) prior to C. difficileinfection. Open circles indicate infection with 10 C. difficile spores;closed squares indicate infection with 10,000 C. difficile spores. Blacktriangles in FIG. 4J indicate an additional experimental arm in whichmice were treated with vancomycin following C. difficile infection.

FIG. 5 shows experimental conditions evaluated in Example 2, the resultsfor which are presented in FIGS. 7-9. Composition E corresponds to amixture of 17 bacterial strains (See e.g., Narushima et al., GutMicrobes 5: 3, 333-339). Composition I corresponds to a mixture ofClostridium scindens, Pseudoflavonifractor capillosus, and Blautiahansenii. “Abx” refers to treatment with any of the antibiotic regimens.

FIG. 6 shows survival of mice over time post infection with C. difficilespores, according to the experimental conditions shown in FIG. 5. Micelosing >20% body weight of baseline were included in mortality numbersin survival curves.

FIGS. 7A-7I show weight of the mice at various times post infection withC. difficile spores. Groups of mice received cefoperazone (Abx)treatment followed by the indicated composition, or no cefoperazone (noAbx), then were administered C. difficile spores. FIG. 7A shows weightof the mice that received no antibiotic treatment. FIG. 7B shows weightof the mice that received cefoperazone treatment. FIG. 7C shows weightof the mice that received cefoperazone treatment followed by vancomycin.FIG. 7D shows weight of the mice that received cefoperazone treatmentfollowed by Composition I. FIG. 7E shows weight of the mice thatreceived cefoperazone treatment followed by Composition E. FIG. 7F showsweight of the mice that received cefoperazone treatment followed bycomposition A. FIG. 7G shows weight of the mice that receivedcefoperazone treatment followed by composition B. FIG. 7H shows weightof the mice that received cefoperazone treatment followed by compositionC. FIG. 7I shows weight of the mice that received cefoperazone treatmentfollowed by composition D.

FIGS. 8A-8C show the load of C. difficile in colony forming units (CFUs)in fecal pellets at various times post infection with C. difficile. FIG.8A shows C. difficile CFU/g feces one-day post infection. FIG. 8B showsC. difficile CFU/g feces 3 days post infection. FIG. 8C shows C.difficile CFU/g feces 8 days post infection.

FIG. 9 shows experimental conditions evaluated in Example 3, the resultsfor which are presented in FIGS. 10-12.

FIG. 10 shows survival of the mice over time post infection with C.difficile spores, according to the experimental conditions shown in FIG.9. Mice losing >20% body weight of baseline were included in mortalitynumbers in survival curves.

FIG. 11 shows weight of the mice at various times post infection with C.difficile spores.

FIG. 12 shows the C. difficile burden in colony forming units (CFUs) infecal pellets collected from mice 1, 3, and 8 days post infection withC. difficile.

FIG. 13 shows the strains of Composition F. The genus-species notationindicates the closest species based on the sequence of the isolatedstrain.

FIG. 14 shows the classification by Clostridium cluster of the strainsin Composition F and their short-chain fatty acid producing abilities.

FIG. 15 shows experimental conditions evaluated in Example 4, theresults for which are presented in FIGS. 16-18. The dosing days arerelative to C. difficile infection. FMT refers to Fecal MatterTransplant with fecal matter isolated from mice or from humans.

FIG. 16 shows survival of the mice over time post infection with C.difficile spores, according to the experimental conditions shown in FIG.15. Mice losing >20% body weight of baseline were included in mortalitynumbers in survival curves.

FIGS. 17A-17H show weight of the mice at various times post infectionwith C. difficile spores. Groups of mice received cefoperazone (Abx)treatment followed by the indicated composition, then were administeredC. difficile spores. FIG. 17A shows weight of the mice that receivedcefoperazone treatment. FIG. 17B shows weight of the mice that receivedcefoperazone treatment followed by FMT with fecal matter from a human.FIG. 17C shows weight of the mice that received cefoperazone treatmentfollowed by FMT with fecal matter from a mouse. FIG. 17D shows weight ofthe mice that received cefoperazone treatment followed by Composition Bon day −1. FIG. 17E shows weight of the mice that received cefoperazonetreatment followed by Composition B on days −2 and −1. FIG. 17F showsweight of the mice that received cefoperazone treatment followed byComposition B on days −2, −1, 1, 2, and 3. FIG. 17G shows weight of themice that received cefoperazone treatment followed by Composition F onday −1. FIG. 17H shows weight of the mice that received cefoperazonetreatment followed by Composition F on days −2, −1, 1, 2, and 3.

FIGS. 18A-18B show the load of C. difficile in colony forming units(CFUs) in fecal pellets at various times post infection with C.difficile. FIG. 18A shows C. difficile CFU/g feces 8 days postinfection. FIG. 18B shows C. difficile CFU/g feces 17 days postinfection.

FIG. 19 shows the strains of Composition G. The genus-species notationindicates the closest species based on the sequence of the isolatedstrain.

FIG. 20 shows experimental conditions evaluated in Example 5, theresults for which are presented in FIGS. 21-23. CompositionB1=Composition B with Bacteroides; Composition B2=Composition B withBacteroides but without Flavonifractor plautii.

FIG. 21 shows survival of the mice over time post infection with C.difficile spores, according to the experimental conditions shown in FIG.20. Mice losing >20% body weight of baseline were included in mortalitynumbers in survival curves.

FIGS. 22A-22J show weight of the mice at various times post infectionwith C. difficile spores. FIG. 22A shows weight of the mice thatreceived vehicle control. FIG. 22B shows weight of the mice thatreceived Composition F. FIG. 22C shows weight of the mice that receivedComposition G. FIG. 22D shows weight of the mice that receivedcefoperazone treatment followed by Composition B. FIG. 22E shows weightof the mice that received cefoperazone treatment followed by CompositionB2 (=Composition B without Flavonifractor plautii and with addedBacteroides). FIG. 22F shows weight of the mice that receivedcefoperazone treatment followed by Composition B1 (=Composition B withBacteroides added). FIG. 22G shows weight of the mice that receivedcefoperazone treatment followed by frozen Composition B. FIG. 22H showsweight of the mice that received cefoperazone treatment followed byethanol treated human fecal samples. FIG. 22I shows weight of the micethat received cefoperazone treatment followed by ethanol treatedComposition B. FIG. 22J shows weight of the mice that receivedcefoperazone treatment followed by Composition J.

FIG. 23 shows the load of C. difficile in colony forming units (CFUs) infecal pellets at various times post infection with C. difficile.

FIG. 24 shows weight of the indicated groups of mice at various timespost infection with C. difficile spores.

FIG. 25 shows experimental conditions evaluated in Example 6, theresults of which are presented in FIGS. 27-29.

FIG. 26 shows the strains in Composition H (SEQ IDNO:14—VE202-13—Anaerotruncus colihominis (Cluster IV); SEQ IDNO:16—VE202-16—Clostridium symbiosum (Cluster XIVa); SEQ IDNO:21-189—Clostridium innocuum (Cluster XVII); SEQ IDNO:82—PE9—Clostridium disporicum (Cluster I); SEQ IDNO:81—PE5—Clostridium bolteae (Cluster XIVa); SEQ IDNO:80—VE202-18—Erysipelatoclostridium ramosum (Cluster XVIII).

FIGS. 27A and 27B shows survival and weight loss of the mice over timepost infection with C. difficile spores, according to the experimentalconditions shown in FIG. 25. Mice losing >20% body weight of baselinewere included in mortality numbers in survival curves. FIG. 29A showssurvival/mortality of mice that received the indicated treatment priorto C. difficile infection. FIG. 29B shows the weight over time of micethat received the indicated treatment prior to C. difficile infection.

FIGS. 28A and 28B show results from the experimental conditions shown inFIG. 25. FIG. 28A shows survival/mortality of mice that received theindicated treatment prior to C. difficile infection. FIG. 28B shows theweight over time of mice that received the indicated treatment prior toC. difficile infection.

FIGS. 29A and 29B show the C. difficile burden in CFU/gram fecescollected from mice that received the indicated treatment prior to C.difficile. FIG. 29A shows C. difficile burden at one-day post C.difficile infection. FIG. 29B shows C. difficile burden at 4 days postC. difficile infection. FIG. 29C shows C. difficile burden at 19 dayspost C. difficile infection.

FIG. 30 shows that Composition B reduced the amount of C. difficileToxin B compared to no treatment controls: “2-1 (Cdiff)” and “2-4(Cdiff)” and FMT. In addition, Composition B reduced the amount of C.difficile Toxin B compared to Composition B with additional spores.

FIG. 31 shows Composition B reduced C. difficile growth in in vitrocompetition experiments. Cultures of C. difficile were incubated in thepresence of B. thetaiotaomicron, C. bifermentans, or Composition B, orin the absence of a competing strain(s) (C. diff only). The quantity ofC. difficile is presented as the percentage of the control (C. diffonly).

FIG. 32 shows that inoculation with Composition B induced the percentageof FoxP3+CD4+ cells (regulatory T cells) in the intestine of germ-freemice as compared to control mice (“GF”).

DETAILED DESCRIPTION OF THE INVENTION

Disclosed herein are compositions comprising purified bacterial strainsand pharmaceutical compositions and food products containing suchcompositions and bacterial strains. Also disclosed are methods oftreating a pathogenic infection, such as Clostridium difficile (C.difficile) infection, in a subject by administering said compositions tothe subject.

Various factors including antibiotic usage can induce dysbiosis of thegastrointestinal tract, which may allow for colonization by pathogenicmicroorganisms, such as C. difficile. Such colonization or pathogenicinfection can lead to a variety of adverse effects in the subjectincluding diarrhea, which is one of the primary symptoms characteristicof C. difficile infection (CDI). In the case of CDI, diarrhea is thoughtto be a result of C. difficile production of Toxin B (also referred toas cytotoxin TcdB), which results in opening of the tight junctionsbetween intestinal epithelial cells, increasing vascular permeability,hemorrhage, and inflammation.

The compositions described herein are effective in the treatment of C.difficile infection. As shown herein, the disclosed compositions areeffective in suppressing the pathogenic effects of C. difficileinfection. The compositions provided herein reduce the amount of C.difficile after infection and thereby provide an effective method foreliminating C. difficile from the body (e.g., the gut). The compositionsprovided herein induce the proliferation and/or accumulation ofregulatory T cells (Tregs), for example when administered to a subject.Remarkably, the compositions disclosed herein have been found to reduceor inhibit production or activity of C. difficile Toxin B and therebyrepresent effective compositions for the treatment or prevention of CDI.The compositions disclosed herein have also been found to inhibit thegrowth and/or survival of C. difficile.

The present disclosure provides compositions comprising purifiedbacterial strains that can be administered to subjects experiencing orhaving experienced a pathogenic infection to treat the infection. Insome embodiments, the compositions may be administered to subjects whomay be at risk for a pathogenic infection. Such subjects includesubjects who previously had pathogenic infections, subjects who havebeen treated with antibiotics and subjects who will undergo a procedurethat will put them at an increased risk for a pathogenic infection(e.g., surgery and/or hospitalization). In some embodiments, thepathogenic infection, is infection by a pathogen that is presentpredominantly in the gut or the intestine. In some embodiments, thepathogen that is present predominantly in the gut or the intestine isClostridium difficile.

In some embodiments, the one or more of the bacterial strains of thecompositions provided herein colonize or recolonize the intestinal tractor parts of the intestinal tract (e.g., the colon or the cecum) of thesubject. Such colonization or recolonization may also be referred to asgrafting. In some embodiments, the one or more of the bacterial strainsof the compositions recolonize the intestinal tract (e.g., the colon orthe cecum) of the subject after the naturally present microbiome hasbeen partially or completely removed, e.g., because of administration ofantibiotics. In some embodiments, the one or more of the bacterialstrains of the compositions colonize a dysbiotic gastrointestinal tract.

In some embodiments, the one or more of the bacterial strains of thecompositions can “outgrow” a pathogen, such as C. difficile. Thus, insome embodiments, if a pathogen (e.g., C. difficile) and one or morebacteria of compositions provided herein are both present in theintestinal tract (e.g., the colon or the cecum), the one or morebacteria of compositions provided herein grow faster (e.g., have ashorter doubling time) than the pathogen, thereby preventing thepathogen from accumulating in the intestinal tract (e.g., the colon orthe cecum). In some embodiments, the faster growth results because theone or more bacteria of the compositions provided herein are better atgrafting in the intestinal tract (e.g., the colon or the cecum). In someembodiments, the faster growth results because the one or more bacteriaof the compositions provided herein are better at metabolizing nutrientspresent in the intestinal tract (e.g., the colon or the cecum). In someembodiments, the compositions of bacterial strains provided hereinprevent or inhibit production of bacterial toxins by the pathogenicinfection, or prevent or inhibit the cytopathic or cytotoxic effects ofsuch bacterial toxins. In some embodiments, the bacterial strains of thecompositions provided herein can treat pathogenic infections, because ofthe synergy between the bacterial strains. Thus, without being limiting,in some embodiments, the combination of the bacterial strains of thecompositions provided herein act synergistically because the combinationof the strains is particularly well-suited to use nutrients in theintestinal tract (e.g., the colon or the cecum), or instance throughmetabolic interactions, and/or because the combination is superior ingrafting (e.g., by providing a favorable microenvironment).

In some embodiments, a pathogenic infection such as C. difficile istreated because the combination of bacterial strains of the compositionsprovided herein is superior in the use of nutrients when compared to thepathogen such as C. difficile, thereby suppressing the growth of thepathogen such as C. difficile. In some embodiments, a pathogenicinfection such as C. difficile is treated because the combination ofbacterial strains of the compositions provided herein is superior ingrafting when compared to the pathogen such as C. difficile, therebysuppressing the growth of the pathogen such as C. difficile. In someembodiments, a pathogenic infection such as C. difficile is treatedbecause the combination of bacterial strains of the compositionsprovided herein is superior in the use of nutrients and in grafting whencompared to the pathogen such as C. difficile, thereby suppressing thegrowth of the pathogen such as C. difficile. In some embodiments, apathogenic infection such as C. difficile is treated because thecombination of bacterial strains of the compositions provided hereininhibits the growth and/or survival of the pathogen such as C.difficile. In some embodiments, a pathogenic infection such as C.difficile is treated because the combination of bacterial strains of thecompositions provided herein induces regulatory T cells (Tregs) in thesubject that results in reduction or elimination of the pathogen such asC. difficile. In some embodiments, a pathogenic infection such as C.difficile is treated because the combination of bacterial strains of thecompositions provided herein inhibits the growth and/or survival of thepathogen and induces regulatory T cells (Tregs) in the subject thatresults in reduction or elimination of the pathogen such as C.difficile.

In some embodiments, the synergistic effect is provided by the capacityof the combination to colonize specific niches in the intestinal tract(e.g., the colon or the cecum). In some embodiments, the synergisticeffect is provided by the capacity of the combination to metabolizespecific nutrients. In some embodiments, the synergistic effect isprovided by the capacity of the combination to provide specificmetabolites to the environment. Such specific metabolites may suppressgrowth of the pathogen and/or stimulate growth of non-pathogens. In someembodiments, the synergistic effect is provided by the capacity of thecombination to provide short-chain fatty acids to the environment. Insome embodiments, the synergistic effect is provided by the capacity ofthe combination to provide specific short-chain fatty acids to theenvironment. In some embodiments, the synergistic effect is provided bythe capacity of the combination to produce butyrate. In someembodiments, the synergistic effect is provided by the capacity of thecombination to produce acetate. In some embodiments, the synergisticeffect is provided by the capacity of the combination to producelactate. In some embodiments, the synergistic effect is provided by thecapacity of the combination to produce propionate. In some embodiments,the synergistic effect is provided by the capacity of the combination toproduce succinate. In some embodiments, the synergistic effect isprovided by the capacity of the combination to produce multiplemetabolites. In some embodiments, the synergistic effect is provided bythe capacity of the combination to produce multiple short-chain fattyacids. In some embodiments, the synergistic effect is provided by thecapacity of the combination to produce both butyrate and acetate. Insome embodiments, the synergistic effect is provided by the capacity ofthe combination to produce both butyrate and lactate. In someembodiments, the synergistic effect is provided by the capacity of thecombination to produce both butyrate and propionate. In someembodiments, the synergistic effect is provided by the capacity of thecombination to produce both butyrate and succinate. In some embodiments,the synergistic effect is provided by the capacity of the combination toproduce butyrate, acetate and additional short-chain fatty acids.

The bacterial strains used in the compositions provided herein generallyare isolated from the microbiome of healthy individuals. In someembodiments, the compositions include strains originating from a singleindividual. In some embodiments, the compositions include strainsoriginating from multiple individuals. In some embodiments, thebacterial strains are obtained from multiple individuals, isolated andgrown up individually. The bacterial compositions that are grown upindividually may subsequently be combined to provide the compositions ofthe disclosure. It should be appreciated that the origin of thebacterial strains of the compositions provided herein is not limited tothe human microbiome from a healthy individual. In some embodiments, thebacterial strains originate from a human with a microbiome in dysbiosis.In some embodiments, the bacterial strains originate from non-humananimals or the environment (e.g., soil or surface water). In someembodiments, the combinations of bacterial strains provided hereinoriginate from multiple sources (e.g., human and non-human animals).

In some embodiments, the bacteria of the compositions provided hereinare anaerobic bacteria. In some embodiments, the bacteria of thecompositions provided herein are obligate anaerobic bacteria. In someembodiments, the bacteria of the compositions provided herein areclostridia. Clostridia may be classified into phylogenetic clusters withother closely related strains and species. (See e.g.,Rajilic-Stojanovic, M., and de Vos, W. M. FEMS Microbiol Rev 38, (2014)996-1047). In general, clostridia are classified as belonging to aspecific cluster based on their 16S rRNA (or 16S rDNA) nucleic acidsequence. Methods for determining the identity of specific bacterialspecies based on their 16S rRNA (or 16S rDNA) nucleic acid sequence arewell known in the art (See e.g., Jumpstart Consortium Human MicrobiomeProject Data Generation Working, G. PLoS One (2012) 7, e39315).

Provided herein are compositions comprising bacterial strains belongingto specific Clostridium clusters that have been found to be effective intreating and/or preventing pathogenic infection (e.g., C. difficileinfection). In some embodiments, at least one of the bacterial strainsof the composition belongs to Clostridium cluster IV. In someembodiments, at least one of the bacterial strains of the compositionbelongs to Clostridium cluster XIVa. In some embodiments, at least oneof the bacterial strains of the composition belongs to Clostridiumcluster XVII. In some embodiments, at least one of the bacterial strainsof the composition belongs to Clostridium cluster I. In someembodiments, at least one of the bacterial strains of the compositionbelongs to Clostridium cluster IX. In some embodiments, at least one ofthe bacterial strains of the composition belongs to Clostridium clusterXIVa and at least one of the bacterial strains belongs to Clostridiumcluster XVII. In some embodiments, at least one of the bacterial strainsof the composition belongs to Clostridium cluster IV and at least one ofthe bacterial strains belongs to Clostridium cluster XVII. In someembodiments, at least one of the bacterial strains of the compositionbelongs to Clostridium cluster IV, at least one of the bacterial strainsbelongs to Clostridium cluster XIVa, and at least one of the bacterialstrains belongs to Clostridium cluster XVII.

In some embodiments, the composition has at least twice as manybacterial strains that belong to Clostridium cluster XIVa when comparedto the bacterial strains that belong to Clostridium cluster IV. In someembodiments, at least two of the bacterial strains of the compositionbelong to Clostridium cluster IV and at least five of the bacterialstrains belong to Clostridium cluster XIVa. In some embodiments, thecomposition has at least twice as many bacterial strains that belong toClostridium cluster XIVa when compared to the bacterial strains thatbelong to Clostridium cluster IV, and the composition has at least onestrain that belongs to Clostridium cluster XVII. In some embodiments, atleast two of the bacterial strains of the composition belong toClostridium cluster IV, at least five of the bacterial strains belongsto Clostridium cluster XIVa, and at least one of the bacterial strainsbelongs to Clostridium cluster XVII.

In some embodiments, the compositions provided herein do not includebacterial strains belonging to Clostridium cluster XVIII. In someembodiments, the compositions provided herein do not include bacterialstrains belonging to Clostridium cluster XVI. In some embodiments, thecompositions provided herein do not include bacterial strains belongingto Clostridium cluster XI. In some embodiments, the compositionsprovided herein do not include bacterial strains belonging toClostridium cluster I.

In one aspect, the disclosure provides bacterial strains comprising a16S rDNA sequence with a nucleic acid sequence selected from the groupconsisting of SEQ ID NOs: 1-83 and 124-159. It should be appreciatedthat SEQ ID NOs: 1-83 and 124-159 may include both full length andpartial 16S rDNA sequences.

In one aspect, the disclosure provides compositions comprising abacterial strain comprising a 16S rDNA sequence with a nucleic acidsequence selected from the group consisting of SEQ ID NOs: 1-83 and124-159. In one aspect, the disclosure provides compositions comprisingas an active ingredient a bacterial strain comprising a 16S rDNAsequence with a nucleic acid sequence selected from the group consistingof SEQ ID NOs: 1-83 and 124-159. It should be appreciated that for allcompositions provided herein, in some embodiments, the bacterial strainor the bacterial strains are the active ingredient of the composition.

It should be appreciated that for all compositions provided herein, insome embodiments, the bacterial strains are purified. Thus, for examplethe disclosure provides purified bacterial strains comprising a 16S rDNAsequence with a nucleic acid sequence selected from the group consistingof SEQ ID NOs: 1-83 and 124-159. In addition, for example, thedisclosure provides compositions comprising purified bacterial strainscomprising a 16S rDNA sequence with a nucleic acid sequence selectedfrom the group consisting of SEQ ID NOs: 1-83 and 124-159. The bacterialstrains disclosed herein originally may have been obtained and purifiedfrom the microbiota of one or more human individuals or obtained fromsources other than the human microbiota, including soil and non-humanmicrobiota. As provided herein, in some embodiments, bacteria isolatedfrom the human microbiota, non-human microbiota, soil, or anyalternative source are purified prior to use in the compositions andmethods provided herein.

In one aspect, the disclosure provides compositions comprising one ormore bacterial strains, wherein the one or more bacterial strainscomprise a 16S rDNA sequence with a nucleic acid sequence selected fromthe group consisting of SEQ ID NOs:1-83 and 124-159. In one aspect, thedisclosure provides compositions comprising one or more bacterialstrains wherein the one or more bacterial strains comprise 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NOs:1-83 and 124-159. Asdiscussed previously, in some embodiments, the bacterial strains arepurified. Thus, in one aspect, the disclosure provides compositionscomprising one or more purified bacterial strains wherein the one ormore purified bacterial strains comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs:1-83 and 124-159.

In one aspect, the disclosure provides compositions comprising two ormore purified bacterial strains wherein the two or more purifiedbacterial strains comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NOs:1-83 and 124-159. As discussed above, in some embodiments,the bacterial strains are the active ingredient of the composition.Thus, in some embodiments, the disclosure provides compositionscomprising as an active ingredient two or more purified bacterialstrains wherein the two or more purified bacterial strains comprise 16SrDNA sequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NOs:1-83 and 124-159.

In one aspect, the disclosure provides bacterial strains andcombinations of bacterial strains that are homologous or have a highpercent of homology with bacterial strains comprising 16S rDNA sequencesselected from the group consisting of SEQ ID NOs:1-83 and 124-159. Asdiscussed previously, in some embodiments, the bacterial strains arepurified. The bacterial strains disclosed herein that have a 16S rDNAsequence with a nucleic acid sequence selected from the group consistingof SEQ ID NOs:1-83 and 124-159 have a high percent of homology (e.g.,greater than 90%) with 16S rDNA sequences of bacterial strains that havebeen described in various databases (See e.g., the National Center forBiotechnology Information). Table 1 and Table 3 provides the closestknown species by homology when the 16S rDNA sequences comprising SEQ IDNOs:1-83 and 124-159 are compared to 16S rDNA sequences of bacterialspecies available in public databases. By way of example, the bacterialstrain comprising a 16S rDNA sequence with SEQ ID NO:1 (also referred toherein as “Strain 71”) disclosed herein has the highest homology with abacterial strain of the species Blautia wexlerae as defined by Accession# NR_044054 (having 16S rDNA sequence SEQ ID NO:94). While the bacterialstrain with SEQ ID NO:1 has homology with other published bacterialstrains as well, the highest homology is with a bacterial strain of thespecies Blautia wexlerae as defined by Accession # NR_044054. In thisparticular example the homology of SEQ ID NO:1 is 96.6% with SEQ IDNO:94 (corresponding to Blautia wexlerae). It should be appreciated thatmultiple bacterial strains disclosed herein may have the highesthomology with the same species. (e.g., both SEQ ID NO:4 and SEQ ID NO:5have the highest homology with a 16S rDNA sequence of a strain of thespecies Blautia hansenii).

It should further be appreciated that the bacterial strains disclosedherein that have a 16S rDNA sequence with a nucleic acid sequenceselected from the group consisting of SEQ ID NOs:1-83 and 124-159, arealso homologous to other strains based on their whole genome sequence,or subset of their whole genome sequence. Homologies based on wholegenome analysis are provided in Table 2 and Table 3.

In one aspect, the disclosure provides compositions comprising one ormore bacterial strains wherein the one or more bacterial strains are ofspecies selected from the group consisting of Clostridium hathewayi,Blautia hansenii, Blautia producta, Blautia producta ATCC 27340,Clostridium bacterium UC5.1-1D4, Blautia coccoides, Eubacteriumcontortum, Eubacterium fissicatena, Sellimona intestinalis, Dracourtellamassiliensis, Dracourtella massiliensis GD1, Ruminococcus torques,Anaerostipes caccae, Clostridium scindens, Marvinbryanta formatexigens,Eisenbergiella tayi, Flavinofractor plautii, Clostridium orbiscindens1_3_50AFAA, Lachnospiraceae bacterium 7_1_58FAA, Subdoligranulum,Anaerotruncus colihominis, Anaerotruncus colihominis DSM 17241,Clostridium symbiosum, Clostridium symbiosum WAL-14163, Clostridiumbolteae, Clostrdium bolteae 90A9, Dorea longicatena, Dorea longicatenaCAG:42, Clostridium innocuum, Erysipelotrichaceae_bacterium_21-3,Blautia wexlerae, Clostridium disporicum, Erysipelatoclostridiumramosum, Pseudoflavinofractor capillosus, Turicibacter sanguinis,Lactobacillus mucosae, Ruminococcus obeum, Megasphaera elsdenii,Acidaminococcus fermentans, Acidaminococcus intestine, Ruminococcusfaecis, Bacteroides cellulosilyticus, Anaerostipes hadrus, Eubacteriumrectale, Ruminococcus champanellensis, Ruminococcus albus,Bifidobacterium bifidum, Blautia luti, Roseburia faecis,Fusicatenibacter saccharivorans, Roseburia faecis, Blautia faecis, Doreaformicigenerans and Bacteroides ovatus.

In some embodiments, the disclosure provides compositions comprising twoor more bacterial strains, wherein the two or more bacterial strains areof species selected from the group consisting of Clostridium hathewayi,Blautia hansenii, Blautia producta, Blautia producta ATCC 27340,Clostridium bacterium UC5.1-1D4, Blautia coccoides, Eubacteriumcontortum, Eubacterium fissicatena, Sellimona intestinalis, Dracourtellamassiliensis, Dracourtella massiliensis GD1, Ruminococcus torques,Anaerostipes caccae, Clostridium scindens, Marvinbryanta formatexigens,Eisenbergiella tayi, Flavinofractor plautii, Clostridium orbiscindens1_3_50AFAA, Lachnospiraceae bacterium 7_1_58FAA, Subdoligranulum,Anaerotruncus colihominis, Anaerotruncus colihominis DSM 17241,Clostridium symbiosum, Clostridium symbiosum WAL-14163, Clostridiumbolteae, Clostrdium bolteae 90A9, Dorea longicatena, Dorea longicatenaCAG:42, Clostridium innocuum, Erysipelotrichaceae_bacterium_21-3,Blautia wexlerae, Clostridium disporicum, Erysipelatoclostridiumramosum, Pseudoflavinofractor capillosus, Turicibacter sanguinis,Lactobacillus mucosae, Ruminococcus obeum, Megasphaera elsdenii,Acidaminococcus fermentans, Acidaminococcus intestine, Ruminococcusfaecis, Bacteroides cellulosilyticus, Anaerostipes hadrus, Eubacteriumrectale, Ruminococcus champanellensis, Ruminococcus albus,Bifidobacterium bifidum, Blautia luti, Roseburia faecis,Fusicatenibacter saccharivorans, Roseburia faecis, Blautia faecis, Doreaformicigenerans and Bacteroides ovatus.

It should be appreciated that the compositions may include multiplestrains of a particular species. Thus, for illustration, a non-limitingexample of the compositions disclosed herein, comprises one strain ofClostridium hathewayi and two strains of Blautia hansenii.

The invention also encompasses compositions comprising bacterial strainsthat are close in homology to and/or fall within the species Clostridiumhathewayi, Blautia hansenii, Blautia producta, Blautia producta ATCC27340, Clostridia bacteria UC5.1-1D4, Blautia coccoides, Eubacteriumcontortum, Eubacterium fissicatena, Sellimona intestinalis, Dracourtellamassiliensis, Dracourtella massiliensis GD1, Ruminococcus torques,Anaerostipes caccae, Clostridium scindens, Marvinbryanta formatexigens,Eisenbergiella tayi, Flavinofractor plautii, Clostridium orbiscindens1_3_50AFAA, Lachnospiraceae bacterium 7_1_58FAA, Subdoligranulum,Anaerotruncus colihominis, Anaerotruncus colihominis DSM 17241,Clostridium symbiosum, Clostridium symbiosum WAL-14163, Clostridiumbolteae, Clostrdium bolteae 90A9, Dorea longicatena, Dorea longicatenaCAG:42, Clostridium innocuum, Erysipelotrichaceae_bacterium_21-3,Blautia wexlerae, Clostridium disporicum, Erysipelatoclostridiumramosum, Pseudoflavinofractor capillosus, Turicibacter sanguinis,Lactobacillus mucosae, Ruminococcus obeum, Megasphaera elsdenii,Acidaminococcus fermentans, Acidaminococcus intestine, Ruminococcusfaecis, Bacteroides cellulosilyticus, Anaerostipes hadrus, Eubacteriumrectale, Ruminococcus champanellensis, Ruminococcus albus,Bifidobacterium bifidum, Blautia luti, Roseburia faecis,Fusicatenibacter saccharivorans, Roseburia faecis, Blautia faecis, Doreaformicigenerans and Bacteroides ovatus. Thus, in one embodiment, thecompositions of the disclosure include one or more bacterial strainscomprising 16S rDNA sequences having at least 97% homology with nucleicacid sequences selected from the group consisting of SEQ ID NOs: 84-123.In some embodiments, the compositions of the disclosure include two ormore bacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NOs:84-123.

In one aspect, the compositions of the disclosure include two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs:1-23 and 124-159. In some embodiments, thecompositions of the disclosure include two or more bacterial strains ofspecies selected from the group consisting of Clostridium hathewayi,Blautia hansenii, Blautia producta, Blautia producta ATCC 27340,Clostridia bacteria UC5.1-1D4, Blautia coccoides, Eubacterium contortum,Eubacterium fissicatena, Sellimona intestinalis, Dracourtellamassiliensis, Dracourtella massiliensis GD1, Ruminococcus torques,Anaerostipes caccae, Clostridium scindens, Marvinbryanta formatexigens,Eisenbergiella tayi, Flavinofractor plautii, Clostridium orbiscindens1_3_50AFAA, Lachnospiraceae bacterium 7_1_58FAA, Subdoligranulum,Anaerotruncus colihominis, Anaerotruncus colihominis DSM 17241,Clostridium symbiosum, Clostridium symbiosum WAL-14163, Clostridiumbolteae, Clostrdium bolteae 90A9, Dorea longicatena, Dorea longicatenaCAG:42, Clostridium innocuum, Erysipelotrichaceae_bacterium_21-3,Blautia wexlerae, Turicibacter sanguinis, Lactobacillus mucosae, andBacteroides ovatus. In some embodiments, the compositions of thedisclosure include two or more purified bacterial strains comprising 16SrDNA sequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO:87, SEQ ID NO:88, SEQ IDNO:89, SEQ ID NO:90, SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:94, SEQ IDNO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:102, SEQ IDNO:103, SEQ ID NO:105, SEQ ID NO:106, SEQ ID NO:108, SEQ ID NO:109, SEQID NO:110, SEQ ID NO:121, and SEQ ID NO:122.

In one aspect, the disclosure provides Composition A (See e.g., FIG. 1,Table A). As shown in FIG. 1, Composition A contains bacterial strainsthat comprise 16S rDNA sequences with nucleic acid sequences: SEQ IDNO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9,SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:23. In someembodiments, the disclosure provides compositions with two or morepurified bacterial strains that comprise 16S rDNA sequences with nucleicacid sequences selected from the group consisting of SEQ ID NO:3, SEQ IDNO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11,SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:23. In some embodiments, thedisclosure provides compositions with five or more purified bacterialstrains that comprise 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ IDNO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12,SEQ ID NO:13, and SEQ ID NO:23. In some embodiments, the disclosureprovides compositions with at least ten purified bacterial strains,wherein the bacterial strains comprise 16S rDNA sequences with nucleicacid sequences SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQID NO:23, respectively. In some embodiments, the disclosure provides acomposition consisting of ten purified bacterial strains, wherein thebacterial strains comprise 16S rDNA sequences with nucleic acidsequences SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ IDNO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ IDNO:23, respectively. In some embodiments, the disclosure provides acomposition essentially consisting of ten purified bacterial strains,wherein the bacterial strains comprise 16S rDNA sequences with nucleicacid sequences SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQID NO:23, respectively. As used herein, essentially consisting of refersto a composition that includes no additional bacterial strains.

In some embodiments, the disclosure provides compositions with bacterialstrains that comprise 16S rDNA sequences having at least 97% homologywith nucleic acid sequences selected from the group consisting of: SEQID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ IDNO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:23. Insome embodiments, the disclosure provides compositions with two or morepurified bacterial strains that comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ IDNO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ IDNO:23. In some embodiments, the disclosure provides compositions withfive or more purified bacterial strains that comprise 16S rDNA having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ IDNO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ IDNO:23. In some embodiments, the disclosure provides compositions with atleast ten purified bacterial strains, wherein the bacterial strainscomprise 16S rDNA sequences having at least 97% homology with nucleicacid sequences SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQID NO:23, respectively. In some embodiments, the disclosure provides acomposition consisting of ten purified bacterial strains, wherein thebacterial strains comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences SEQ ID NO:3, SEQ ID NO:4, SEQ IDNO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12,SEQ ID NO:13, and SEQ ID NO:23, respectively. In some embodiments, thedisclosure provides a composition essentially consisting of ten purifiedbacterial strains, wherein the bacterial strains comprise 16S rDNAsequences having at least 97% homology with nucleic acid sequences SEQID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ IDNO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:23,respectively.

The bacterial strains in Composition A are related to the followingbacterial species: Clostridium hathewayi, Blautia hansenii, Blautiaproducta, Blautia coccoides, Eubacterium contortum, Eubacteriumfissicatena, Anaerostipes caccae, Clostridium scindens, Marvinbryantaformatexigens, and Eisenbergiella tayi (See e.g., Table 1). It should beappreciated that multiple bacterial strains of the compositionsdisclosed herein can have the same related bacterial species. Forinstance, the bacterial strains having 16S rDNA sequences with nucleicacid sequences SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:7 all have Blautiahansenii as related species. In some embodiments, the disclosureprovides compositions with two or more bacteria of species selected fromthe group consisting of Clostridium hathewayi, Blautia hansenii, Blautiaproducta, Blautia coccoides, Eubacterium contortum, Eubacteriumfissicatena, Anaerostipes caccae, Clostridium scindens, Marvinbryantaformatexigens, and Eisenbergiella tayi. In some embodiments, thedisclosure provides compositions with two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:87, SEQ ID NO:88, SEQ ID NO:89, SEQ ID NO:99, SEQ ID NO:103, SEQ IDNO:105, SEQ ID NO:106, SEQ ID NO:108, SEQ ID NO:109, and SEQ ID NO:121.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:23. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11,SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:23. In some embodiments, thecomposition comprises two or more purified bacterial strains comprising16S rDNA sequences having at least 97% homology with nucleic acidsequences selected from the group consisting of SEQ ID NO:3, SEQ IDNO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12,SEQ ID NO:13, and SEQ ID NO:23. In some embodiments, the compositioncomprises two or more purified bacterial strains comprising 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ IDNO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ IDNO:23. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ IDNO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:23. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:3, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12,SEQ ID NO:13, and SEQ ID NO:23. In some embodiments, the compositioncomprises two or more purified bacterial strains comprising 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ IDNO:5; SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:13.

Each of the bacterial strains of Composition A are BaiCD+, meaning thatthe bacterial strains encode, or are predicted to encode, the bileinducible operon gene BaiCD and/or a protein with stereospecificNAD(H)-dependent 3-oxo-Δ⁴-cholenoic acid oxidoreductase activity. TheBaiCD status of a bacterial strain can be determined for instance by PCR(See e.g., Wells et al. Clin Chim Acta (2003) May; 331(1-2):127-34).Furthermore, each of the strains of Composition A are classified asbelonging to Clostridium cluster XIVa. In some embodiments, thedisclosure provides compositions comprising two or more bacterialstrains, wherein the bacterial strains are BaiCD+ strains. In someembodiments, the disclosure provides compositions comprising two or morebacterial strains, wherein the bacterial strains are BaiCD+ and belongto Clostridium cluster XIVa. In some embodiments of the compositionscomprising two or more bacterial strains that are BaiCD+ strains andthat belong to Clostridium cluster XIVa, the compositions do not includebacterial strains that belong to Clostridium cluster IV.

In some embodiments, the disclosure provides compositions with two ormore purified bacterial strains that comprise 16S rDNA sequences havingat least 97% homology with nucleic acid sequences selected from thegroup consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7,SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, andSEQ ID NO:23, wherein all the bacterial strains belong to Clostridiumcluster XIVa.

TABLE A Composition A SEQ_03-5—Clostridium_hathewayi (XIVa)*SEQ_04-7—Blautia_hansenii (XIVa)* SEQ_05-10—Blautia_hansenii (XIVa)*SEQ_07-59—Blautia_producta/Blautia_coccoides (XIVa)SEQ_08-79—Blautia_hansenii (XIVa)*SEQ_09-VE202-21—Eubacterium_contortum/ Eubacterium_fissicatena (XIVa)*SEQ_11-VE202-9—Anaerostipes_caccae (XIVa)*SEQ_12-VE202-26—Clostridium_scindens (XIVa)*SEQ_13-136—Marvinbryantia_formatexigens (XIVa)*SEQ_23-VE202-29—Eisenbergiella_tayi (XIVa)* *BaiCD⁺

In one aspect, the disclosure provides Composition B (See e.g., FIG. 1,Table B). As shown in FIG. 1, Composition B contains bacterial strainsthat comprise 16S rDNA sequences with nucleic acid sequences: SEQ IDNO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159. In someembodiments, the compositions include two or more purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ IDNO:21, and SEQ ID NOs: 124-159. In some embodiments, the compositionsinclude two or more purified bacterial strains comprising 16S rDNAsequences with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21. In some embodiments, thecompositions include two or more purified bacterial strains comprising16S rDNA sequences with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs: 124-159. In some embodiments, the compositionsinclude two or more purified bacterial strains comprising 16S rDNAsequences with nucleic acid sequences selected from the group consistingof SEQ ID NO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ IDNO:141, SEQ ID NO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the compositions include five or more purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159. In some embodiments, thecompositions include five or more purified bacterial strains comprising16S rDNA sequences with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21. In someembodiments, the compositions include five or more purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NOs: 124-159. In someembodiments, the compositions include five or more purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NO: 124, SEQ ID NO:129, SEQID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152and SEQ ID NO:157.

In some embodiments, the compositions include at least eight purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159, respectively. In someembodiments, the compositions include at least eight purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQID NO:21, respectively. In some embodiments, the compositions include atleast eight purified bacterial strains comprising 16S rDNA sequenceswith nucleic acid sequences selected from the group consisting of SEQ IDNOs: 124-159, respectively. In some embodiments, the compositionsinclude at least eight purified bacterial strains comprising 16S rDNAsequences with nucleic acid sequences selected from the group consistingof SEQ ID NO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ IDNO:141, SEQ ID NO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the composition consists of at least eight purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159, respectively. In someembodiments, the composition consists of at least eight purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ IDNO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21, respectively. Insome embodiments, the composition consists of eight purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NOs:124-159, respectively.In some embodiments, the compositions consists of at least eightpurified bacterial strains comprising 16S rDNA sequences with nucleicacid sequences SEQ ID NO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ IDNO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the composition essentially consists of at leasteight purified bacterial strains comprising 16S rDNA sequences withnucleic acid sequences selected from the group consisting of SEQ IDNO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159,respectively. In some embodiments, the composition essentially consistsof at least eight purified bacterial strains comprising 16S rDNAsequences with nucleic acid sequences SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQID NO:21, respectively. In some embodiments, the composition essentiallyconsists of at least eight purified bacterial strains comprising 16SrDNA sequences with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs:124-159, respectively. In some embodiments, thecomposition essentially consists of at least eight purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequences SEQ IDNO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQID NO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the compositions include eight purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ IDNO:21, and SEQ ID NOs: 124-159, respectively. In some embodiments, thecompositions include eight purified bacterial strains comprising 16SrDNA sequences with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21,respectively. In some embodiments, the compositions include eightpurified bacterial strains comprising 16S rDNA sequences with nucleicacid sequences selected from the group consisting of SEQ ID NOs:124-159, respectively. In some embodiments, the compositions includeeight purified bacterial strains comprising 16S rDNA sequences withnucleic acid sequences selected from the group consisting of SEQ ID NO:124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ IDNO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the composition consists of eight purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159, respectively. In someembodiments, the composition consists of eight purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequences SEQ IDNO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:19, SEQ ID NO:20, and SEQ ID NO:21, respectively. In someembodiments, the composition consists of eight purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NOs:124-159, respectively.In some embodiments, the compositions consists of eight purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences SEQ ID NO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137,SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the composition essentially consists of eightpurified bacterial strains comprising 16S rDNA sequences with nucleicacid sequences selected from the group consisting of SEQ ID NO:10, SEQID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159, respectively. In someembodiments, the composition essentially consists of eight purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ IDNO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21, respectively. Insome embodiments, the composition essentially consists of eight purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NOs:124-159,respectively. In some embodiments, the composition essentially consistsof eight purified bacterial strains comprising 16S rDNA sequences withnucleic acid sequences SEQ ID NO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152, and SEQ IDNO:157.

In one aspect, the disclosure provides a composition that containsbacterial strains that comprise 16S rDNA sequences with nucleic acidsequences: SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22, and SEQ ID NOs:124-159. In one aspect, the disclosure provides a composition thatcontains bacterial strains that comprise 16S rDNA sequences with nucleicacid sequences: SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, and SEQ ID NO:22. In oneaspect, the disclosure provides a composition that contains bacterialstrains that comprise 16S rDNA sequences with nucleic acid sequences:SEQ ID NOs: 124-159. In one aspect, the disclosure provides acomposition that contains bacterial strains that comprise 16S rDNAsequences with nucleic acid sequences: SEQ ID NO: 124, SEQ ID NO:129,SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ IDNO:152 and SEQ ID NO:157.

In some embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:21, SEQ ID NO:22, SEQ ID NOs: 124-145, and SEQ ID NOs: 152-159. Insome embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:21, and SEQ ID NO:22. In some embodiments, the compositions includetwo or more purified bacterial strains comprising 16S rDNA sequenceswith nucleic acid sequences selected from the group consisting of SEQ IDNO:22, SEQ ID NOs: 124-145, and SEQ ID NOs: 152-159. In someembodiments, the compositions include two or more purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NO: 124, SEQ ID NO:129, SEQID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152,and SEQ ID NO:157.

In some embodiments, the compositions include five or more purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:21, SEQ ID NO:22, and SEQ ID NOs: 124-145, and SEQ ID NOs: 152-159.In some embodiments, the compositions include five or more purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:21, and SEQ ID NO:22. In some embodiments, the compositions includefive or more purified bacterial strains comprising 16S rDNA sequenceswith nucleic acid sequences selected from the group consisting of SEQ IDNO: 22, SEQ ID NOs: 124-145, and SEQ ID NOs: 152-159. In someembodiments, the compositions include five or more purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NO: 124, SEQ ID NO:129, SEQID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152and SEQ ID NO:157.

In some embodiments, the compositions include at least eight purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:21, SEQ ID NO:22, SEQ ID NOs: 124-145, and SEQ ID NOs: 152-159,respectively. In some embodiments, the compositions include at leasteight purified bacterial strains comprising 16S rDNA sequences withnucleic acid sequences selected from the group consisting of SEQ IDNO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:19, SEQ ID NO:21, and SEQ ID NO:22, respectively. In someembodiments, the compositions include at least eight purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NO:22, SEQ ID NOs: 124-145,and SEQ ID NOs: 152-159. In some embodiments, the compositions includefive or more purified bacterial strains comprising 16S rDNA sequenceswith nucleic acid sequences selected from the group consisting of SEQ IDNO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQID NO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the composition consists of at least eight purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:21, SEQ ID NO:22, SEQ ID NOs: 124-145, and SEQ ID NOs: 152-159,respectively. In some embodiments, the composition consists of at leasteight purified bacterial strains comprising 16S rDNA sequences withnucleic acid sequences SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ IDNO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, and SEQ ID NO:22,respectively. In some embodiments, the composition consists of at leasteight purified bacterial strains comprising 16S rDNA sequences withnucleic acid sequences selected from the group consisting of SEQ ID NO:22, SEQ ID NOs: 124-145, and SEQ ID NOs: 152-159. In some embodiments,the composition consists of at least eight purified bacterial strainscomprising 16S rDNA sequences with nucleic acid sequences SEQ ID NO:124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ IDNO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the composition essentially consists of at leasteight purified bacterial strains comprising 16S rDNA sequences withnucleic acid sequences sequences selected from the group consisting ofSEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NOs: 124-145, and SEQID NOs: 152-159, respectively. In some embodiments, the compositionessentially consists of at least eight purified bacterial strainscomprising 16S rDNA sequences with nucleic acid sequences SEQ ID NO:10,SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19,SEQ ID NO:21, and SEQ ID NO:22, respectively. In some embodiments, thecomposition essentially consists of at least eight purified bacterialstrains comprising 16S rDNA sequences with nucleic acid sequencesselected from the group consisting of SEQ ID NO: 22, SEQ ID NOs:124-145, and SEQ ID NOs: 152-159. In some embodiments, the compositionessentially consists of at least eight purified bacterial strainscomprising 16S rDNA sequences with nucleic acid sequences SEQ ID NO:124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ IDNO:146, SEQ ID NO:152 and SEQ ID NO:157.

In one aspect, the disclosure provides compositions that containbacterial strains that comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof: SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ IDNO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO: 124-159.In one aspect, the disclosure provides compositions that containbacterial strains that comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences: SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQID NO:21. In one aspect, the disclosure provides compositions thatcontain bacterial strains that comprise 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of: SEQ ID NOs: 124-159. In one aspect, the disclosureprovides compositions that contain bacterial strains that comprise 16SrDNA sequences having at least 97% homology with nucleic acid sequencesSEQ ID NO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ IDNO:141, SEQ ID NO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159. Insome embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21. In some embodiments, thecompositions include two or more purified bacterial strains comprising16S rDNA sequences having at least 97% homology with nucleic acidsequences selected from the group consisting of SEQ ID NOs: 124-159. Insome embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ IDNO:141, SEQ ID NO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the compositions include five or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159. Insome embodiments, the compositions include five or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21. In some embodiments, thecompositions include five or more purified bacterial strains comprising16S rDNA sequences having at least 97% homology with nucleic acidsequences selected from the group consisting of SEQ ID NOs: 124-159. Insome embodiments, the compositions include five or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ IDNO:141, SEQ ID NO:146, SEQ ID NO:152 and SEQ ID NO:157.

In some embodiments, the compositions include at least eight purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO: 124-159,respectively. In some embodiments, the compositions include at leasteight purified bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21,respectively. In some embodiments, the compositions include at leasteight purified bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs: 124-159. In some embodiments, the compositionsinclude at least eight purified bacterial strains comprising 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO: 124, SEQ ID NO:129, SEQID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152and SEQ ID NO:157.

In some embodiments, the composition consists of at least eight purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NOs: 124-159,respectively. In some embodiments, the composition consists of at leasteight purified bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:20, and SEQ ID NO:21, respectively. In some embodiments, thecomposition consists of at least eight purified bacterial strainscomprising 16S rDNA sequences having at least 97% homology with nucleicacid sequences selected from the group consisting of SEQ ID NOs:124-159,respectively. In some embodiments, the composition consists of at leasteight purified bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences SEQ ID NO: 124, SEQ IDNO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQID NO:152 and SEQ ID NO:157.

In some embodiments, the composition essentially consists of at leasteight purified bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO:124-159, respectively. In some embodiments, the composition essentiallyconsists of at least eight purified bacterial strains comprising 16SrDNA sequences having at least 97% homology with nucleic acid sequencesSEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21, respectively. In someembodiments, the composition essentially consists of at least eightpurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs: 124-159, respectively. In some embodiments,the composition essentially consists of at least eight purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences SEQ ID NO: 124, SEQ ID NO:129, SEQID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152and SEQ ID NO:157.

In one aspect, the disclosure provides a composition that containsbacterial strains that comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences: SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ IDNO:22, SEQ ID NO: 124-145, and SEQ ID NOs: 152-159. In one aspect, thedisclosure provides a composition that contains bacterial strains thatcomprise 16S rDNA sequences having at least 97% homology with nucleicacid sequences: SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, and SEQ ID NO:22. In oneaspect, the disclosure provides a composition that contains bacterialstrains that comprise 16S rDNA sequences having at least 97% homologywith nucleic acid sequences: SEQ ID NO:22, SEQ ID NO: 124-145, and SEQID NOs: 152-159.

In some embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22, and SEQ ID NO: 124-145, andSEQ ID NOs: 152-159. In some embodiments, the compositions include twoor more purified bacterial strains comprising 16S rDNA sequences havingat least 97% homology with nucleic acid sequences selected from thegroup consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ IDNO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, and SEQ ID NO:22. Insome embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO: 22, SEQ ID NO: 124-145, and SEQ ID NOs: 152-159.

In some embodiments, the compositions include five or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO: 124-145, and SEQ IDNOs: 152-159. In some embodiments, the compositions include five or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, and SEQ ID NO:22. In someembodiments, the compositions include five or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ ID NO:20, SEQ ID NO: 124-145, and SEQ ID NOs: 152-159.

In some embodiments, the compositions include at least eight purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO: 124-145, and SEQ IDNOs: 152-159, respectively. In some embodiments, the compositionsinclude at least eight purified bacterial strains comprising 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, and SEQID NO:22, respectively. In some embodiments, the compositions include atleast eight purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO: 22, SEQ ID NO: 124-145, and SEQ IDNOs: 152-159.

In some embodiments, the composition consists of at least eight purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ IDNO:22, SEQ ID NO: 124-145, and SEQ ID NOs: 152-159, respectively. Insome embodiments, the composition consists of at least eight purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, and SEQID NO:22, respectively. In some embodiments, the composition consists ofat least eight purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences SEQ ID NO: 22,SEQ ID NO: 124-145, and SEQ ID NOs: 152-159.

In some embodiments, the composition essentially consists of at leasteight purified bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:21, SEQ ID NO:22, SEQ ID NO: 124-145, and SEQ ID NOs: 152-159,respectively. In some embodiments, the composition essentially consistsof at least eight purified bacterial strains comprising 16S rDNAsequences having at least 97% homology with nucleic acid sequences SEQID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:19, SEQ ID NO:21, and SEQ ID NO:22, respectively. In someembodiments, the composition essentially consists of at least eightpurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences SEQ ID NO: 22, SEQ ID NO:124-145, and SEQ ID NOs: 152-159.

In one aspect, the disclosure provides a composition that containsbacterial strains that comprise 16S rDNA sequences with nucleic acidsequences: SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ IDNO:22, and SEQ ID NO: 124-159. In one aspect, the disclosure provides acomposition that contains bacterial strains that comprise 16S rDNAsequences with nucleic acid sequences: SEQ ID NO:10, SEQ ID NO:14, SEQID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ IDNO:20, SEQ ID NO:21, and SEQ ID NO:22. In one aspect, the disclosureprovides a composition that contains bacterial strains that comprise 16SrDNA sequences with nucleic acid sequences: SEQ ID NO:18, SEQ ID NO:22,and SEQ ID NO: 124-159.

In some embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22,and SEQ ID NO: 124-159. In some embodiments, the compositions includetwo or more purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ IDNO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ IDNO:21, and SEQ ID NO:22. In some embodiments, the compositions includetwo or more purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:18, SEQ ID NO:22, and SEQ ID NO:124-159.

In some embodiments, the compositions include five or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22,and SEQ ID NOs: 124-159. In some embodiments, the compositions includefive or more purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ IDNO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ IDNO:21, and SEQ ID NO:22. In some embodiments, the compositions includefive or more purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:18, SEQ ID NO:22, and SEQ ID NOs:124-159.

In some embodiments, the compositions include at least ten purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22,and SEQ ID NOs: 124-159, respectively. In some embodiments, thecompositions include at least ten purified bacterial strains comprising16S rDNA sequences having at least 97% homology with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ IDNO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO:22, respectively. Insome embodiments, the compositions include at least ten purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO: 18, SEQ ID NO: 22, and SEQ ID NOs: 124-159.

In some embodiments, the composition consists of ten purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, and SEQID NO: 124-159, respectively. In some embodiments, the compositionconsists of ten purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences SEQ ID NO:10,SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO:22,respectively. In some embodiments, the composition consists of tenpurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:18, SEQ ID NO:22, SEQ ID NOs: 124-159,respectively.

In some embodiments, the composition essentially consists of tenpurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21,SEQ ID NO:22, and SEQ ID NO: 124-159, respectively. In some embodiments,the composition essentially consists of ten purified bacterial strainscomprising 16S rDNA sequences having at least 97% homology with nucleicacid sequences SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21,and SEQ ID NO:22, respectively. In some embodiments, the compositionessentially consists of ten purified bacterial strains comprising 16SrDNA sequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO:18, SEQ ID NO:22, andSEQ ID NO: 124-159, respectively.

The bacterial strains in Composition B are related to the followingbacterial species: Flavinofractor plautii, Lachnospiraceae, bacterium7_1_58FAA, Subdoligranulum Anaerotruncus colihominis, Eubacteriumfissicatena, Ruminococcus torques Clostridium symbiosum, Clostridiumbolteae, Dorea longicatena, Blautia producta, Clostridium innocuum, andErysipelotrichaceae_bacterium_21-3 (See e.g., Table 2).

Selected strains were subjected to whole genome sequencing using aPacBio Biosciences platform (Menlo Park, Calif.) and sequences wereassembled into whole genomes (Table 3). The 16S rDNA sequences wereidentified using Prokka and Barrnap. It was found that several strainscontained more than one 16S sequence. All identified 16S rRNA genenucleotide sequences for each strain were then clustered at 97% identityusing the usearch (v 5.2.236) algorithm and the cluster seed sequencewas selected as the representative sequence for each Composition Bstrain (The Consensus 16S sequence: column labeled “*Consensus SEQ ID #of 16S region as determined by WGS” in Table 3). Table 3 providesidentification of the indicated strains included in Composition B basedon Sanger sequencing of the 16S region as well as on whole genomesequencing (WGS). The closest species of the bacterial strains wereidentified both by comparison to a 16S database (column labeled:“Closest species based on Consensus SEQ ID # of 16S region as comparedwith 16S database”) and to whole genome databases (column labeled:“Closest species based on WGS compared versus WG databases).

Based on identification of 16S sequences through whole genomesequencing, and by comparing these sequences with 16S databases, thebacterial strains in Composition B are related to the followingbacterial species: Clostridium bolteae, Anaerotruncus colihominis,Dracourtella massiliensis, Clostridium symbiosum Blautia producta, Dorealongicatena Clostridium innocuum and Flavinofractor plautii (see, e.g.,Table 3).

Based on whole genome sequencing and comparing of the whole genome towhole genome databases, the bacterial strains in Composition B are mostclosely related to the following bacterial species: Clostridium bolteae90A9, Anaerotruncus colihominis DSM 17241, Dracourtella massiliensisGD1, Clostridium symbiosum WAL-14163, Clostridium bacterium UC5.1-1D4,Dorea longicatena CAG:42, Erysipelotrichaceae bacterium 21_3, andClostridium orbiscindens 1_3_50AFAA (see, e.g., Table 3).

It should be appreciated that multiple strains of the compositionsdisclosed herein can have the same related bacterial species. Forinstance, the bacterial strains comprising 16S rDNA sequences withnucleic acid sequences SEQ ID NO 18, SEQ ID NO:20 and SEQ ID NO:22 allhave Dorea longicatena as related bacterial species. In someembodiments, the disclosure provides compositions with two or morebacteria selected from the group consisting of Flavinofractor plautii,Lachnospiraceae, bacterium 7_1_58FAA, Subdoligranulum Anaerotruncuscolihominis, Eubacterium fissicatena, Ruminococcus torques Clostridiumsymbiosum, Clostridium bolteae, Dorea longicatena, Blautia producta,Clostridium innocuum and Erysipelotrichaceae_bacterium_21-3. In someembodiments, the disclosure provides compositions with two or morebacteria selected from the group consisting of Flavinofractor plautii,Anaerotruncus colihominis, Eubacterium fissicatena, Clostridiumsymbiosum, Clostridium bolteae, Dorea longicatena, Blautia producta, andClostridium innocuum. In some embodiments, the disclosure providescompositions that include two or more purified bacterial strainscomprising 16S rDNA sequences having at least 97% homology with nucleicacid sequences selected from the group consisting of SEQ ID NO:93, SEQID NO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:102, SEQ ID NO:106, SEQID NO:110, and SEQ ID NO:122.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, and SEQ ID NOs:124-159. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ IDNO:22. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NOs: 124-159. In some embodiments, the compositioncomprises two or more purified bacterial strains comprising 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO: 124, SEQ ID NO:129, SEQID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152and SEQ ID NO:157.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22, and SEQ ID NO:124-159. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:21, and SEQ IDNO:22. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:18, SEQ ID NO:22, and SEQ ID NO: 124-159. Insome embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:18, SEQ ID NO:22, SEQ ID NO: 124, SEQ ID NO:129, SEQ IDNO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152, andSEQ ID NO:157.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO:124-159. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, and SEQ IDNO:21. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:18 and SEQ ID NO: 124-159. In some embodiments,the composition comprises two or more purified bacterial strainscomprising 16S rDNA sequences having at least 97% homology with nucleicacid sequences selected from the group consisting of SEQ ID NO:18, SEQID NO: 124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141,SEQ ID NO:146, SEQ ID NO:152, and SEQ ID NO:157

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22, and SEQ ID NO: 124-145, andSEQ ID NO: 151-159. In some embodiments, the composition comprises twoor more purified bacterial strains comprising 16S rDNA sequences havingat least 97% homology with nucleic acid sequences selected from thegroup consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ IDNO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, and SEQ ID NO:22. Insome embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:22, SEQ ID NO: 124-145, and SEQ ID NO: 151-159. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ ID NO:124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ IDNO:152, and SEQ ID NO:157

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO: 124-159. Insome embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21. In some embodiments, thecomposition comprises two or more purified bacterial strains comprising16S rDNA sequences having at least 97% homology with nucleic acidsequences selected from the group consisting of SEQ ID NOs: 124-159.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NOs: 124-145, and SEQID NOs: 152-159. In some embodiments, the composition comprises two ormore purified bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, and SEQ ID NO:21. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ ID NO:18, SEQ ID NOs: 124-145, and SEQ ID NOs: 152-159.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21,SEQ ID NO:22, SEQ ID NOs: 157-159, and SEQ ID NOs:141-156. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:10, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQID NO:22. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:18, SEQ ID NO:22, SEQ ID NOs: 157-159, and SEQID NO:141-156.

Each of the bacteria of Composition B are BaiCD− strains, meaning thatthe strains do not encode and/or are not predicted to encode the bileinducible operon gene baiCD and/or a protein with stereospecificNAD(H)-dependent 3-oxo-Δ⁴-cholenoic acid oxidoreductase activity. Insome embodiments, the disclosure provides compositions comprising two ormore bacteria, wherein the bacteria are BaiCD− strains. The strains ofComposition B are classified as belonging to Clostridium clusters IV,XIVa, and XVII. In some embodiments, the disclosure provides two or morebacterial strains, wherein the bacteria are BaiCD− strains and belong toClostridium clusters IV, XIVa, or XVII. In some embodiments, thedisclosure provides two or more bacterial strains, wherein the bacteriaare BaiCD− strains and belong to Clostridium clusters IV or XVII. Insome embodiments, the disclosure provides two or more bacterial strains,wherein the bacteria are BaiCD− strains and belong to Clostridiumclusters XIVa or XVII. In some embodiments, the disclosure provides twoor more bacterial strains, wherein the bacteria are BaiCD− strains andbelong to Clostridium clusters IV or XIVa. In some embodiments, thedisclosure provides two or more bacterial strains, wherein the bacteriaare BaiCD− strains and belong to Clostridium cluster IV. In someembodiments, the disclosure provides two or more bacterial strains,wherein the bacteria are BaiCD− strains and belong to Clostridiumcluster XIVa. In some embodiments, the disclosure provides two or morebacterial strains, wherein the bacteria are BaiCD− strains and belong toClostridium clusters XVII. In some embodiments, the disclosure providestwo or more bacterial strains, wherein the bacteria are BaiCD− strainsand belong to Clostridium clusters IV, XIVa, and XVII and do not belongto Clostridium clusters XVI or XVIII.

In some embodiments, the disclosure provides two or more bacterialstrains wherein the bacterial strains are spore forming bacterialstrains. In some embodiments, the disclosure provides two or morebacterial strains wherein the bacteria are spore formers and wherein thebacterial strains comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:21, SEQ ID NOs124-140, and SEQ ID NO: 152-156. In some embodiments, the disclosureprovides two or more bacterial strains wherein the bacteria are sporeformers and wherein the bacterial strains comprise 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:17, andSEQ ID NO:21. In some embodiments, the disclosure provides two or morebacterial strains wherein the bacteria are spore formers and wherein thebacterial strains comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NOs: 124-140, and SEQ ID NOs: 152-156.

In some embodiments, the disclosure provides two or more bacterialstrains wherein the bacteria include both spore formers and non-sporeformers. In some embodiments, the disclosure provides two or morebacterial strains wherein the bacteria include both spore formers andnon-spore formers, and wherein the spore forming bacterial strainscomprise two or more purified bacterial strains comprising 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO:14, SEQ ID NO:16, SEQ IDNO:17, SEQ ID NO:21, SEQ ID NOs: 124-140, and SEQ ID NOs: 152-156. Insome embodiments, the disclosure provides two or more bacterial strainswherein the bacteria include both spore formers and non-spore formers,and wherein the spore forming bacterial strains comprise two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:17, and SEQ IDNO:21. In some embodiments, the disclosure provides two or morebacterial strains wherein the bacteria include both spore formers andnon-spore formers, and wherein the spore forming bacterial strainscomprise two or more purified bacterial strains comprising 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NOs: 124-140 and SEQ ID NO:152-156.

TABLE B Composition B SEQ_10-211—Flavonifractor_plautii (IV)SEQ_14-VE202-13—Anaerotruncus_colihominis (IV)SEQ_15-VE202-14—Eubacterium_fissicatena (XIVa)SEQ_16-VE202-16—Clostridium_symbiosum (XIVa)SEQ_17-VE202-7—Clostridium_bolteae (XIVa) SEQ_19-16—Blautia_producta(XIVa) SEQ_20-170—Dorea_longicatena (XIVa)SEQ_21-189—Clostridium_innocuum (XVII)

In some embodiments, the compositions include one or more bacterialspecies from the Bacteroides genus. In some embodiments, thecompositions include one or more bacterial species selected from thegroup consisting of B. acidifaciens, B. caccae, B. coprocola, B.coprosuis, B. eggerthii, B. finegoldii, B. fragilis, B. helcogenes, B.intestinalis, B. massiliensis, B. nordii, B. ovatus, B.thetaiotaomicron, B. vulgatus, B. plebeius, B. uniformis B. salyersai,B. pyogenes, B. goldsteinii, B. dorei, and B. johnsonii. In someembodiments, the compositions include Bacteroides ovatus. In someembodiments, the Bacteroides ovatus has a 16S rDNA sequence comprisingSEQ ID NO:83. In some embodiments, the Bacteroides ovatus has a 16S rDNAsequence having at least 97% homology with a nucleic acid sequencecomprising SEQ ID NO:83. In some embodiments, the Bacteroides ovatus hasa 16S rDNA sequence having at least 97% homology with a nucleic acidsequence comprising SEQ ID NO:101.

While not being limited to a specific mechanism it is thought that theinclusion of a Bacteroides species in the bacterial compositionsdisclosed herein increases the ability to sense and adapt to nutrientavailability or influence the host immune system so that it becomes moreeffective in fighting pathogens (e.g., C. difficile). In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO: 124-159, and SEQ ID NO:83.In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO:83. (CompositionB1, See e.g., Table B1). In some embodiments, the composition comprisestwo or more purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NOs: 124-159, and SEQ ID NO: 83.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO: 124-145, SEQ ID NO:152-159, and SEQ ID NO:83. In some embodiments, the compositioncomprises two or more purified bacterial strains comprising 16S rDNAsequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ IDNO:22, and SEQ ID NO:83. In some embodiments, the composition comprisestwo or more purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NOs: 124-145, SEQ ID NO: 152-159, and SEQID NO: 83.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22,SEQ ID NO: 124-159, and SEQ ID NO:83. In some embodiments, thecomposition comprises two or more purified bacterial strains comprising16S rDNA sequences having at least 97% homology with nucleic acidsequences selected from the group consisting of SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ IDNO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22 and SEQ ID NO:83. Insome embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NOs: 124-159, SEQ ID NO: 18, SEQ ID NO: 22, and SEQ ID NO: 83.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16s rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ IDNO:101, SEQ ID NO:102, SEQ ID NO:106, SEQ ID NO:110, and SEQ ID NO:122.It should also be appreciated that in some embodiments, the compositionsdisclosed herein do not include bacterial species from the Bacteroidesgenus.

TABLE B1 Composition B1 SEQ_10-211—Flavonifractor_plautii (IV)SEQ_14-VE202-13—Anaerotruncus_colihominis (IV)SEQ_15-VE202-14—Eubacterium_fissicatena (XIVa)SEQ_16-VE202-16—Clostridium_symbiosum (XIVa)SEQ_17-VE202-7—Clostridium_bolteae (XIVa) SEQ_20-170—Dorea_longicatena(XIVa) SEQ_19-16—Blautia_producta (XIVa) SEQ_21-189—Clostridium_innocuum(XVII) SEQ_83 Bacteroides ovatus

In some embodiments, the compositions disclosed herein do not includeClostridium orbiscindens 1_3_50AFAA, Flavinofractor plautii,Subdoligranulum or Lachnospiraceae bacterium 7_1_58FAA. In someembodiments, the compositions disclosed herein do not includeClostridium orbiscindens 1_3_50AFAA. In some embodiments, thecompositions disclosed herein do not include Flavinofractor plautii. Insome embodiments, the compositions disclosed herein do not includeSubdoligranulum. In some embodiments, the compositions disclosed hereindo not include Lachnospiraceae bacterium 7_1_58FAA.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:14, SEQ ID NO: 15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO: 124-156, wherein thecomposition does not include a bacterial strain comprising a 16s rDNAsequence having at least 97% homology with a nucleic acid sequence ofSEQ ID NO:10 and SEQ ID NOs: 157-159. In some embodiments, thecomposition comprises two or more purified bacterial strains comprising16S rDNA sequences having at least 97% homology with nucleic acidsequences selected from the group consisting of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQ IDNO:21, wherein the composition does not include a bacterial straincomprising a 16s rDNA sequence having at least 97% homology with anucleic acid sequence of SEQ ID NO:10. In some embodiments, thecomposition comprises two or more purified bacterial strains comprising16S rDNA sequences having at least 97% homology with nucleic acidsequences selected from the group consisting of SEQ ID NOs: 124-156,wherein the composition does not include a bacterial strain comprising a16S rDNA sequence having at least 97% homology with a nucleic acidsequence of SEQ ID NOs:157-159.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19,SEQ ID NO:21, SEQ ID NO:22, and SEQ ID NOs: 124-146 and SEQ ID NO:152-156, wherein the composition does not include a bacterial straincomprising a 16S rDNA sequence having at least 97% homology with anucleic acid sequence of SEQ ID NO:10 and SEQ ID NOs: 157-159. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:21, and SEQ ID NO:22, wherein the composition does not include abacterial strain comprising a 16S rDNA sequence having at least 97%homology with a nucleic acid sequence of SEQ ID NO:10. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:22, SEQ ID NOs: 124-146, and SEQ ID NOs: 152-156, wherein thecomposition does not include a bacterial strain comprising a 16S rDNAsequence having at least 97% homology with a nucleic acid sequence ofSEQ ID NOs:157-159.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, and SEQ ID NOs:124-156, wherein the composition does not include a bacterial straincomprising a 16S rDNA sequence having at least 97% homology with anucleic acid sequence of SEQ ID NO:10 and SEQ ID NOs: 157-159. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ IDNO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO:22, wherein thecomposition does not include a bacterial strain comprising a 16S rDNAsequence having at least 97% homology with a nucleic acid sequence ofSEQ ID NO:10. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:18, SEQ ID NO:22, and SEQ ID NOs: 124-159,wherein the composition does not include a bacterial strain comprising a16S rDNA sequence having at least 97% homology with a nucleic acidsequence of SEQ ID NOs: 157-159.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:102, SEQ IDNO:106, SEQ ID NO:110, and SEQ ID NO:122, wherein the composition doesnot include a bacterial strain comprising a 16S rDNA sequence having atleast 97% homology with a nucleic acid sequence of SEQ ID NO:93.

In some embodiments, the compositions include one or more bacterialspecies from the Bacteroides genus and do not include Clostridiumorbiscindens 1_3_50AFAA, Flavinofractor plautii, Subdoligranulum orLachnospiraceae bacterium 7_1_58FAA. (Composition B2, See e.g., TableB2). In some embodiments, the compositions include Bacteroides ovatusand do not include Clostridium orbiscindens 1_3_50AFAA, Flavinofractorplautii, Subdoligranulum or Lachnospiraceae bacterium 7_1_58FAA.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19,SEQ ID NO:20, SEQ ID NO:21 SEQ ID NO:83, and SEQ ID NOs: 124-156,wherein the composition does not include a bacterial strain comprising a16S rDNA sequence having at least 97% homology with a nucleic acidsequence of SEQ ID NO:10 and SEQ ID NOs: 157-159. In some embodiments,the composition comprises two or more purified bacterial strainscomprising 16S rDNA sequences having at least 97% homology with nucleicacid sequences selected from the group consisting of SEQ ID NO:14, SEQID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ IDNO:21 and SEQ ID NO:83, wherein the composition does not include abacterial strain comprising a 16S rDNA sequence having at least 97%homology with a nucleic acid sequence of SEQ ID NO:10. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:83 and SEQ ID NOs: 124-156, wherein the composition does not includea bacterial strain comprising a 16S rDNA sequence having at least 97%homology with a nucleic acid sequence of SEQ ID NOs: 157-159.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19,SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:83, SEQ ID NOs: 124-145, and SEQID NOs: 152-156, wherein the composition does not include a bacterialstrain comprising a 16S rDNA sequence having at least 97% homology witha nucleic acid sequence of SEQ ID NO:10 and SEQ ID NOs: 157-159. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:21, SEQ ID NO:22 and SEQ ID NO:83, wherein the composition does notinclude a bacterial strain comprising a 16S rDNA sequence having atleast 97% homology with a nucleic acid sequence of SEQ ID NO:10. In someembodiments, the composition comprises two or more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:22 SEQ ID NO:83, SEQ ID NOs: 124-145, and SEQ ID NOs: 152-156,wherein the composition does not include a bacterial strain comprising a16S rDNA sequence having at least 97% homology with a nucleic acidsequence of SEQ ID NOs: 157-159.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18,SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:83,and SEQ ID NO: 124-156, wherein the composition does not include abacterial strain comprising a 16S rDNA sequence having at least 97%homology with a nucleic acid sequence of SEQ ID NO:10 and SEQ ID NOs:157-159. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17,SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22 andSEQ ID NO:83, wherein the composition does not include a bacterialstrain comprising a 16S rDNA sequence having at least 97% homology witha nucleic acid sequence of SEQ ID NO:10. In some embodiments, thecomposition comprises two or more purified bacterial strains comprising16S rDNA sequences having at least 97% homology with nucleic acidsequences selected from the group consisting of SEQ ID NO:22, SEQ IDNO:83, and SEQ ID NO: 124-156, wherein the composition does not includea bacterial strain comprising a 16S rDNA sequence having at least 97%homology with a nucleic acid sequence of SEQ ID NOs: 157-159.

In some embodiments, the composition comprises two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:101, SEQ IDNO:102, SEQ ID NO:106, SEQ ID NO:110, and SEQ ID NO:122, wherein thecomposition does not include a bacterial strain comprising a 16S rDNAsequence having at least 97% homology with a nucleic acid sequence ofSEQ ID NO:93.

TABLE B2 Composition B2 SEQ_14-VE202-13—Anaerotruncus_colihominis (IV)SEQ_15-VE202-14—Eubacterium_fissicatena (XIVa)SEQ_16-VE202-16—Clostridium_symbiosum (XIVa) SEQ_17-VE202-7—Clostridium_bolteae (XIVa) SEQ_20-170—Dorea_longicatena (XIVa)SEQ_19-16—Blautia_producta (XIVa) SEQ_21-189—Clostridium_innocuum (XVII)SEQ_83 Bacteroides ovatus

In one aspect, the disclosure provides Composition C (See e.g., FIG. 1,Table C). As shown in FIG. 1, Composition C contains bacteria that havethe following 16S rDNA sequences: SEQ ID NO:12, SEQ ID NO:3, SEQ IDNO:5, SEQ ID NO:1, SEQ ID NO:7, SEQ ID NO:18, SEQ ID NO:21, SEQ IDNO:10, SEQ ID NO:14, and SEQ ID NO:16. In some embodiments, thedisclosure provides compositions with two or more purified bacterialstrains that have 16S rDNA sequences selected from the group consistingof SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:7, SEQID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:14, and SEQ ID NO:16. Insome embodiments, the compositions include four or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:7, SEQID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:14, and SEQ ID NO:16. Insome embodiments, the compositions include at least ten purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences selected from the group consisting of SEQ ID NO:12, SEQ IDNO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:7, SEQ ID NO:18, SEQ ID NO:21,SEQ ID NO:10, SEQ ID NO:14, and SEQ ID NO:16. In some embodiments, thecomposition consists of ten purified bacterial strains comprising 16SrDNA sequences with nucleic acid sequences SEQ ID NO:12, SEQ ID NO:3,SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:7, SEQ ID NO:18, SEQ ID NO:21, SEQID NO:10, SEQ ID NO:14, and SEQ ID NO:16, respectively. In someembodiments, the composition consists essentially of ten purifiedbacterial strains comprising 16S rDNA sequences with nucleic acidsequences SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ IDNO:7, SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:14, and SEQ IDNO:16, respectively.

In some embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:7, SEQID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:14, and SEQ ID NO:16. Insome embodiments, the compositions include four or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:7, SEQID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:14, and SEQ ID NO:16. Insome embodiments, the compositions include at least ten purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:7, SEQID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:14, and SEQ ID NO:16. Insome embodiments, the composition consists of ten purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ IDNO:1, SEQ ID NO:7, SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ IDNO:14, and SEQ ID NO:16, respectively. In some embodiments, thecomposition essentially consists of ten purified bacterial strainscomprising 16S rDNA sequences having at least 97% homology with nucleicacid sequences SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQID NO:7, SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:14, and SEQID NO:16, respectively.

The bacterial strains in Composition C are related to the followingspecies: Clostridium scindens, Clostridium hathewayi, Blautia hansenii,Blautia wexlerae, Blautia producta, Blautia coccoides, Dorealongicatena, Clostridium innocuum, Flavonifractor plautii,Lachnospiraceae bacterium 7_1_58FAA, Subdoligranulum, Anaerotruncuscolihominis, and Clostridium symbiosum. In some embodiments, thedisclosure provides compositions with two or more bacterial strains ofspecies selected from the group consisting of Clostridium scindens,Clostridium hathewayi, Blautia hansenii, Blautia wexlerae, Blautiaproduct, Blautia coccoides, Dorea longicatena, Clostridium innocuum,Flavonifractor plautii, Lachnospiraceae bacterium 7_1_58FAA,Subdoligranulum, Anaerotruncus colihominis, and Clostridium symbiosum.In some embodiments, the disclosure provides compositions that includetwo or more purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:87, SEQ ID NO:93, SEQ ID NO:94, SEQ IDNO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:103, SEQ IDNO:105, SEQ ID NO:106, and SEQ ID NO:122.

In some embodiments, the compositions disclosed herein do not includeFlavinofractor plautii, Subdoligranulum or Lachnospiraceae bacterium7_1_58FAA. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQID NO:7, SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:14, and SEQ ID NO:16,wherein the composition does not include a bacterial strain comprising a16S rDNA sequence having at least 97% homology with a nucleic acidsequence of SEQ ID NO:10. In some embodiments, the composition comprisestwo or more purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:87, SEQ ID NO:94, SEQ ID NO:95, SEQ IDNO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:103, SEQ ID NO:105, SEQ IDNO:106, and SEQ ID NO:122, wherein the composition does not include abacterial strain comprising a 16S rDNA sequence having at least 97%homology with a nucleic acid sequence of SEQ ID NO:93.

The strains of Composition C include both BaiCD+ strains and Bai CD−strains. In some embodiments, the disclosure provides compositionscomprising two or more bacteria, wherein one or more bacteria are BaiCD+strains and one or more bacteria are BaiCD-strains. In some embodimentsof the one or more bacteria that are BaiCD+ strains are selected frombacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, and SEQ ID NO:7.In some embodiments the one or more bacteria that are BaiCD− strains areselected from bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:14,and SEQ ID NO:16. In some embodiments of the one or more bacteria thatare BaiCD+ strains are selected from the bacterial species Clostridiumscindens, Clostridium hathewayi, Blautia hansenii, Blautia wexlerae,Blautia product, and Blautia coccoides. In some embodiments of the oneor more bacteria that are BaiCD− strains are selected from the bacterialspecies Dorea longicatena, Clostridium innocuum, Flavonifractor plautii,or Lachnospiraceae bacterium 7_1_58FAA, Anaerotruncus colihominis, andClostridium symbiosum. The clostridial strains of Composition C areclassified as belonging to Clostridium clusters IV, XIVa, and XVII. Insome embodiments, the disclosure provides two or more bacterial strains,wherein the bacteria are BaiCD− strains and BaiCD+ strains and belong toClostridium clusters IV, XIVa, or XVII. In some embodiments, thedisclosure provides two or more bacterial strains, wherein the bacteriaare BaiCD− strains and BaiCD+ strains and belong to Clostridium clustersXIVa or XVII. In some embodiments, the disclosure provides two or morebacterial strains, wherein the bacteria are BaiCD− strains and BaiCD+strains and belong to Clostridium clusters IV or XIVa.

TABLE C Composition C SEQ_12-VE202-26—Clostridium_scindens (XIVa)*SEQ_03-5—Clostridium_hathewayi (XIVa)* SEQ_05-10—Blautia_hansenii(XIVa)* SEQ_01-71—Blautia_wexlerae (XIVa)*SEQ_07-59—Blautia_producta/Blautia_coccoides (XIVa)*SEQ_18-148—Dorea_longicatena (XIVa) SEQ_21-189—Clostridium_innocuum(XVII) SEQ_10-211—Flavonifractor_plautii (IV)SEQ_14-VE202-13—Anaerotruncus_colihominis (IV)SEQ_16-VE202-16—Clostridium_symbiosum) (XIVa) *BaiCD+

In one aspect, the disclosure provides Composition D (See e.g., FIG. 1,Table D). As shown in FIG. 1, Composition D contains bacteria that havethe following 16S rDNA sequences: SEQ ID NO:12, SEQ ID NO:3, SEQ IDNO:5, SEQ ID NO:1, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:21, SEQ IDNO:10, SEQ ID NO:2, and SEQ ID NO:6. In some embodiments, the disclosureprovides compositions with two or more purified bacterial strains thathave 16S rDNA sequences selected from the group consisting of SEQ IDNO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:14, SEQ IDNO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:2, and SEQ ID NO:6. In someembodiments, the disclosure provides compositions that include three ormore purified bacterial strains comprising 16S rDNA sequences withnucleic acid sequences selected from the group consisting of SEQ IDNO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:14, SEQ IDNO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:2, and SEQ ID NO:6. In someembodiments, the disclosure provides compositions that include at leastten purified bacterial strains comprising 16S rDNA sequences withnucleic acid sequences selected from the group consisting of SEQ IDNO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:14, SEQ IDNO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:2, and SEQ ID NO:6. In someembodiments, the disclosure provides a composition that consists of tenpurified bacterial strains comprising 16S rDNA sequences with nucleicacid sequences SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQID NO:14, SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:2, and SEQID NO:6, respectively. In some embodiments, the disclosure provides acomposition that consists essentially of ten purified bacterial strainscomprising 16S rDNA sequences with nucleic acid sequences SEQ ID NO:12,SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:14, SEQ ID NO:18, SEQID NO:21, SEQ ID NO:10, SEQ ID NO:2, and SEQ ID NO:6, respectively Insome embodiments, the disclosure provides compositions that include twoor more purified bacterial strains comprising 16S rDNA sequences havingat least 97% homology with nucleic acid sequences selected from thegroup consisting of SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1,SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:2, andSEQ ID NO:6. In some embodiments, the disclosure provides compositionsthat include three or more purified bacterial strains comprising 16SrDNA sequences having at least 97% homology with nucleic acid sequencesselected from the group consisting of SEQ ID NO:12, SEQ ID NO:3, SEQ IDNO:5, SEQ ID NO:1, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:21, SEQ IDNO:10, SEQ ID NO:2, and SEQ ID NO:6. In some embodiments, the disclosureprovides compositions that include at least ten more purified bacterialstrains comprising 16S rDNA sequences having at least 97% homology withnucleic acid sequences selected from the group consisting of SEQ IDNO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQ ID NO:14, SEQ IDNO:18, SEQ ID NO:21, SEQ ID NO:10, SEQ ID NO:2, and SEQ ID NO:6. In someembodiments, the disclosure provides a composition that consists of tenpurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences SEQ ID NO:12, SEQ ID NO:3, SEQID NO:5, SEQ ID NO:1, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:21, SEQ IDNO:10, SEQ ID NO:2, and SEQ ID NO:6, respectively. In some embodiments,the disclosure provides a composition that consists essentially of tenpurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences SEQ ID NO:12, SEQ ID NO:3, SEQID NO:5, SEQ ID NO:1, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:21, SEQ IDNO:10, SEQ ID NO:2, and SEQ ID NO:6, respectively.

The bacterial strains in Composition D are related to the followingbacteria: Clostridium scindens, Clostridium hathewayi, Blautia hansenii,Blautia wexlerae, Anaerotruncus colihominis, Dorea longicatena,Clostridium innocuum, Flavonifractor plautii, Lachnospiraceae bacterium7_1_58FAA, Subdoligranulum, Turicibacter sanguinis, and Lactobacillusmucosae. In some embodiments, the disclosure provides compositions withtwo or more bacterial strains of species selected from the groupconsisting of Clostridium scindens, Clostridium hathewayi, Blautiahansenii, Blautia wexlerae, Anaerotruncus colihominis, Dorealongicatena, Clostridium innocuum, Erysipelotrichaceae_bacterium_21-3,Flavonifractor plautii, Lachnospiraceae bacterium 7_1_58FAA,Turicibacter sanguinis, and Lactobacillus mucosae. In some embodiments,the disclosure provides compositions that include two or more purifiedbacterial strains comprise 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:87, SEQ ID NO:90, SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:94,SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, and SEQ IDNO:105.

In some embodiments, the compositions disclosed herein do not includeFlavinofractor plautii, Subdoligranulum or Lachnospiraceae bacterium7_1_58FAA. In some embodiments, the composition comprises two or morepurified bacterial strains comprising 16S rDNA sequences having at least97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:1, SEQID NO:14, SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:2, and SEQ ID NO:6,wherein the composition does not include a bacterial strain comprising a16S rDNA sequence having at least 97% homology with a nucleic acidsequence of SEQ ID NO:10. In some embodiments, the composition comprisestwo or more purified bacterial strains comprising 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:87, SEQ ID NO:90, SEQ ID NO:91, SEQ IDNO:94, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, and SEQID NO:105, wherein the composition does not include a bacterial straincomprising a 16S rDNA sequence having at least 97% homology with anucleic acid sequence of SEQ ID NO:93.

The strains of Composition D include both BaiCD₊ strains and Bai CD−strains. In some embodiments, the disclosure provides compositionscomprising two or more bacteria, wherein one or more bacteria are BaiCD+strains and one or more bacteria are BaiCD-strains. In some embodimentsof the one or more bacteria that are BaiCD+ strains are selected frombacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:5, and SEQ ID NO:1. In someembodiments the one or more bacteria that are BaiCD− strains areselected from bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:18, SEQ ID NO:21, SEQ ID NO:10, and SEQ IDNO:14. In some embodiments of the one or more bacteria that are BaiCD+strains are selected from the bacterial species Clostridium scindens,Clostridium hathewayi, Blautia hansenii, and Blautia wexlerae. In someembodiments of the one or more bacteria that are BaiCD− strains areselected from the bacterial species Dorea longicatena, Clostridiuminnocuum, Flavonifractor plautii, and Anaerotruncus colihominis. TheClostridial strains of Composition D are classified as belonging toClostridium clusters IV, XIVa, and XVII. In some embodiments, thedisclosure provides two or more bacterial strains, wherein the bacteriaare BaiCD− strains and BaiCD+ strains and belong to Clostridium clustersIV, XIVa, or XVII. In some embodiments, the disclosure provides two ormore bacterial strains, wherein the bacteria are BaiCD− strains andBaiCD+ strains and belong to Clostridium clusters XIVa or XVII. In someembodiments, the disclosure provides two or more bacterial strains,wherein the bacteria are BaiCD− strains and BaiCD+ strains and belong toClostridium clusters IV or XIVa.

Composition D includes the non-Clostridium strains Turicibactersanguinis and Lactobacillus mucosae. In some embodiments, the disclosureprovides compositions comprising two or more bacteria, wherein thecomposition includes both Clostridium strains and non-Clostridiumstrains. In some embodiments, the non-clostridium strains are themembers of the genus Lactobacillus. Members of the genus Lactobacillusinclude, without limitation L. acetotolerans, L. acidifarinae, L.acidipiscis, L. acidophilus, L. agilis, L. algidus, L. alimentarius, L.amylolyticus, L. amylophilus, L. amylotrophicus, L. amylovorus, L.animalis, L. antri, L. apodemi, L. aviarius, L. bifermentans, L. brevis,L. buchneri, L. camelliae, L. casei, L. catenaformis, L. ceti, L.coleohominis, L. collinoides, L. composti, L. concavus, L. coryniformis,L. crispatus, L. crustorum, L. curvatus, L. delbrueckii subsp.bulgaricus, L. delbrueckii subsp. delbrueckii, L. delbrueckii subsp.lactis, L. dextrinicus, L. diolivorans, L. equi, L. equigenerosi, L.farraginis, L. farciminis, L. fermentum, L. fornicalis, L. fructivorans,L. frumenti, L. fuchuensis, L. gallinarum, L. gasseri, L. gastricus, L.ghanensis, L. graminis, L. hammesii, L. hamsteri, L. harbinensis, L.hayakitensis, L. helveticus, L. hilgardii, L. homohiochii, L. iners, L.ingluviei, L. intestinalis, L. jensenii, L. johnsonii, L. kalixensis, L.kefiranofaciens, L. kefiri, L. kimchii, L. kitasatonis, L. kunkeei, L.leichmannii, L. lindneri, L. malefermentans, L. mali, L. manihotivorans,L. mindensis, L. mucosae, L. murinus, L. nagelii, L. namurensis, L.nantensis, L. oligofermentans, L. oris, L. panis, L. pantheris, L.parabrevis, L. parabuchneri, L. paracasei, L. paracollinoides, L.parafarraginis, L. parakefiri, L. paralimentarius, L. paraplantarum, L.pentosus, L. perolens, L. plantarum, L. pontis, L. protectus, L.psittaci, L. rennini, L. reuteri, L. rhamnosus, L. rimae, L. rogosae, L.rossiae, L. ruminis, L. saerimneri, L. sakei, L. salivarius, L.sanfranciscensis, L. satsumensis, L. secaliphilus, L. sharpeae, L.siliginis, L. spicheri, L. suebicus, L. thailandensis, L. ultunensis, L.vaccinostercus, L. vaginalis, L. versmoldensis, L. vini, L. vitulinus,L. zeae, and L. zymae. In some embodiments, the non-clostridium strainis Lactobacillus mucosae. In some embodiments, the non-clostridiumstrain is Lactobacillus mucosae. In some embodiments, the Lactobacillusmucosae has a 16S rDNA sequence comprising SEQ ID NO:2. In someembodiments, the Lactobacillus mucosae has a 16S rDNA sequence having atleast 97% homology with a nucleic acid comprising SEQ ID NO:2. In someembodiments, the Lactobacillus mucosae has a 16S rDNA sequence having atleast 97% homology with a nucleic acid comprising SEQ ID NO:91.

In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein the composition includes both Clostridiumstrains and non-Clostridium strains. In some embodiments, thenon-clostridium strains are members of the genus Turicibacter. In someembodiments, the non-clostridium strain is Turicibacter sanguinis. Insome embodiments, the Turicibacter sanguinis has a 16S rDNA sequencecomprising SEQ ID NO:6. In some embodiments, the Turicibacter sanguinishas a 16S rDNA sequence having at least 97% homology with a nucleic acidcomprising SEQ ID NO:6. In some embodiments, the Turicibacter sanguinishas a 16S rDNA sequence having at least 97% homology with a nucleic acidcomprising SEQ ID NO:90.

In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein the composition includes both Clostridiumstrains and non-Clostridium strains. In some embodiments, thenon-Clostridium strains are Lactobacillus mucosae and Turicibactersanguinis.

In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein the composition does not includeLactobacillus. In some embodiments, the disclosure provides compositionscomprising two or more bacteria, wherein the composition does notinclude Turicibacter. In some embodiments, the disclosure providescompositions comprising two or more bacteria, wherein the compositiondoes not include Lactobacillus or Turicibacter. In some embodiments, thedisclosure provides compositions comprising two or more bacteria,wherein the composition only includes clostridia strains. In someembodiments, the disclosure provides compositions comprising two or morebacteria, wherein the composition only includes clostridia strainsbelonging to Clostridium cluster IV, XIVa or XVII strains. In someembodiments, the disclosure provides compositions comprising two or morebacteria, wherein the composition does not include Clostridium clusterXI strains.

In some embodiments, the disclosure provides compositions comprising twoor more purified bacterial strains selected from the group consistingof: Clostridium scindens, Pseudoflavonifractor capillosus, and Blautiahansenii. In some embodiments, the compositions disclosed herein do notinclude Clostridium scindens, Pseudoflavonifractor capillosus, orBlautia hansenii.

TABLE D Composition D SEQ_12-VE202-26—Clostridium_scindens (XIVa)*SEQ_03-5—Clostridium_hathewayi (XIVa)* SEQ_05-10—Blautia_hansenii(XIVa)* SEQ_01-71—Blautia_wexlerae (XIVa)*SEQ_14-VE202-13—Anaerotruncus_colihominis (IV)SEQ_18-148—Dorea_longicatena (XIVa) SEQ_21-189—Clostridium_innocuum(XVII) SEQ_10-211—Flavonifractor_plautii (IV)SEQ_02-102—Turicibacter_sanguinis (non-Clostridium)SEQ_06-40—Lactobacillus_mucosae (non-Clostridium) *BaiCD+

In one aspect, the disclosure provides Composition F (See e.g., FIGS. 13and 14, and Tables F1 and F2). As shown in FIG. 13, Composition Fcontains bacteria that have the following 16S rDNA sequences: SEQ IDNO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ IDNO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ IDNO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ IDNO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ IDNO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ IDNO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ IDNO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ IDNO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ IDNO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ IDNO:69, SEQ ID NO:70, SEQ ID NO:71, SEQ ID NO:72, SEQ ID NO:73, SEQ IDNO:74, SEQ ID NO:75, SEQ ID NO:76, SEQ ID NO:77, SEQ ID NO:78, and SEQID NO:79.

In some embodiments, the disclosure provides compositions with two ormore purified bacterial strains that have 16S rDNA sequences selectedfrom the group consisting of SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26,SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31,SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36,SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41,SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46,SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51,SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56,SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61,SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66,SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71,SEQ ID NO:72, SEQ ID NO:73, SEQ ID NO:74, SEQ ID NO:75, SEQ ID NO:76,SEQ ID NO:77, SEQ ID NO:78, and SEQ ID NO:79.

In some embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28,SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33,SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38,SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43,SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48,SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53,SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58,SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63,SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68,SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71, SEQ ID NO:72, SEQ ID NO:73,SEQ ID NO:74, SEQ ID NO:75, SEQ ID NO:76, SEQ ID NO:77, SEQ ID NO:78,and SEQ ID NO:79.

The bacterial strains in Composition F are related to the followingbacteria: Dorea longicatena, Ruminococcus obeum, Megasphaera elsdenii,Acidaminococcus fermentans, Acidaminococcus intestine, Megasphaeraelsdenii, Ruminococcus faecis, Bacteroides cellulosilyticus,Anaerostipes hadrus, Ruminococcus obeum, Flavonifractor plautii,Eubacterium rectale, Flavonifractor plautii, Megasphaera elsdenii,Eubacterium rectale, Ruminococcus champanellensis, Ruminococcus albus,Ruminococcus champanellensis, Ruminococcus faecis, Bifidobacteriumbifidum, Anaerostipes hadrus, Anaerostipes hadrus, Anaerostipes hadrus,Eubacterium rectale, Ruminococcus faecis, Blautia luti, Ruminococcusfaecis, Anaerostipes hadrus, Anaerostipes hadrus, Ruminococcus faecis,Eubacterium rectale, Eubacterium rectale, Anaerostipes hadrus,Ruminococcus faecis, Ruminococcus faecis, Dorea longicatena, Roseburiafaecis, Blautia luti, Fusicatenibacter saccharivorans, Fusicatenibactersaccharivorans, Roseburia faecis, Megasphaera elsdenii, Eubacteriumrectale, Eubacterium rectale, Roseburia faecis, Blautia faecis,Fusicatenibacter saccharivorans, and Dorea formicigenerans.

In some embodiments, the disclosure provides compositions with two ormore bacterial strains of species selected from the group consisting ofDorea longicatena, Ruminococcus obeum, Megasphaera elsdenii,Acidaminococcus fermentans, Acidaminococcus intestine, Megasphaeraelsdenii, Ruminococcus faecis, Bacteroides cellulosilyticus,Anaerostipes hadrus, Ruminococcus obeum, Flavonifractor plautii,Eubacterium rectale, Flavonifractor plautii, Megasphaera elsdenii,Eubacterium rectale, Ruminococcus champanellensis, Ruminococcus albus,Ruminococcus champanellensis, Ruminococcus faecis, Bifidobacteriumbifidum, Anaerostipes hadrus, Anaerostipes hadrus, Anaerostipes hadrus,Eubacterium rectale, Ruminococcus faecis, Blautia luti, Ruminococcusfaecis, Anaerostipes hadrus, Anaerostipes hadrus, Ruminococcus faecis,Eubacterium rectale, Eubacterium rectale, Anaerostipes hadrus,Ruminococcus faecis, Ruminococcus faecis, Dorea longicatena, Roseburiafaecis, Blautia luti, Fusicatenibacter saccharivorans, Fusicatenibactersaccharivorans, Roseburia faecis, Megasphaera elsdenii, Eubacteriumrectale, Eubacterium rectale, Roseburia faecis, Blautia faecis,Fusicatenibacter saccharivorans, and Dorea formicigenerans.

In some embodiments, the disclosure provides compositions that includetwo or more purified bacterial strains comprise 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:92, SEQ IDNO:93, SEQ ID NO:96, SEQ ID NO:97, SEQ ID NO:99, SEQ ID NO:100, SEQ IDNO:104, SEQ ID NO:107, SEQ ID NO:111, SEQ ID NO:112, SEQ ID NO:113, SEQID NO:114, SEQ ID NO:115, SEQ ID NO:116, SEQ ID NO:117, SEQ ID NO:118,SEQ ID NO:119, and SEQ ID NO:120. It should be appreciated that multiplestrains of the compositions disclosed herein can have the same relatedbacterial species. For instance, Composition F includes 12 strains thathave Eubacterium rectale as the closest related species.

In some embodiments, at least one of the bacterial strains of thecomposition belongs to Clostridium cluster IV. In some embodiments, atleast one of the bacterial strains of the composition belongs toClostridium cluster XIVa. In some embodiments, at least one of thebacterial strains of the composition belongs to Clostridium cluster IX.In some embodiments, at least one of the bacterial strains of thecomposition belongs to Clostridium cluster IV. In some embodiments, atleast one of the bacterial strains of the composition belongs toClostridium cluster XIVa and at least one of the bacterial strainsbelongs to Clostridium cluster IX. In some embodiments, at least one ofthe bacterial strains of the composition belongs to Clostridium clusterIV and at least one of the bacterial strains belongs to Clostridiumcluster IX. In some embodiments, at least one of the bacterial strainsof the composition belongs to Clostridium cluster IV, at least one ofthe bacterial strains belongs to Clostridium cluster XIVa, and at leastone of the bacterial strains belongs to Clostridium cluster IX. In someembodiments, the compositions provided herein do not include bacterialstrains belonging to Clostridium cluster XVIII. In some embodiments, thecompositions provided herein do not include bacterial strains belongingto Clostridium cluster XVI or XVIII.

Composition F includes non-clostridium bacterial strains. In someembodiments, the disclosure provides compositions comprising two or morebacteria, wherein the composition includes both Clostridium strains andnon-clostridium strains. In some embodiments, the non-clostridiumstrains are the members of the genus Bacteroides. In some embodiments,the non-clostridium strain is Bacteroides cellulosilyticus. In someembodiments, the non-clostridium strains are the members of the genusBifidobacterium. In some embodiments, the non-clostridium strain isBifidobacterium bifidum. In some embodiments, the disclosure providescompositions comprising two or more bacteria, wherein the compositionincludes both Clostridium strains and non-Clostridium strains, andwherein the non-Clostridium strains are Bacteroides cellulosilyticus andBifidobacterium bifidum.

In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein the composition does not include Bacteroides.In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein the composition does not includeBifidobacterium. In some embodiments, the disclosure providescompositions comprising two or more bacteria, wherein the compositiondoes not include Bacteroides and does not include Bifidobacterium. Insome embodiments, the disclosure provides compositions comprising two ormore bacteria, wherein the composition does not include non-Clostridiumstrains. In some embodiments, the disclosure provides compositionscomprising two or more bacteria, wherein the composition only includesclostridia strains belonging to Clostridium cluster IV, XIVa or XVIIstrains. In some embodiments, the disclosure provides compositionscomprising two or more bacteria, wherein the composition does notinclude Clostridium cluster XI strains.

TABLE F1 Composition F SEQ_NO StrainID Genus_species SEQ_24 YK96Dorea_longicatena SEQ_25 YK101 Ruminococcus_obeum SEQ_26 YK110Megasphaera_elsdenii SEQ_27 YK149 Acidaminococcus_fermentans/Acidaminococcus_intestini SEQ_28 YK154 Megasphaera_elsdenii SEQ_29 YK36Ruminococcus_faecis SEQ_30 YK95 Bacteroides_cellulosilyticus SEQ_31 YK32Anaerostipes_hadrus SEQ_32 YK64 Ruminococcus_obeum SEQ_33 YK73Flavonifractor_plautii SEQ_34 YK87 Eubacterium_rectale SEQ_35 YK105Flavonifractor_plautii SEQ_36 YK153 Megasphaera_elsdenii SEQ_37 YK163Eubacterium_rectale SEQ_38 YK191 Ruminococcus_champanellensis/Ruminococcus_albus SEQ_39 YK99 Ruminococcus_champanellensis SEQ_40 YK55Ruminococcus_faecis SEQ_41 YK75 Bifidobacterium_bifidum SEQ_42 YK90Anaerostipes_hadrus SEQ_43 YK30 Anaerostipes_hadrus SEQ_44 YK31Anaerostipes_hadrus SEQ_45 YK12 Eubacterium_rectale SEQ_46 YK27Ruminococcus_faecis SEQ_47 YK28 Blautia_luti SEQ_48 YK29Ruminococcus_faecis SEQ_49 YK33 Anaerostipes_hadrus SEQ_50 YK34Anaerostipes_hadrus SEQ_51 YK35 Ruminococcus_faecis SEQ_52 YK51Eubacterium_rectale SEQ_53 YK52 Eubacterium_rectale SEQ_54 YK54Anaerostipes_hadrus SEQ_55 YK56 Ruminococcus_faecis SEQ_56 YK57Ruminococcus_faecis SEQ_57 YK58 Dorea_longicatena SEQ_58 YK65Roseburia_faecis SEQ_59 YK67 Blautia_luti SEQ_60 YK69Fusicatenibacter_saccharivorans SEQ_61 YK70Fusicatenibacter_saccharivorans SEQ_62 YK71 Roseburia_faecis SEQ_63 YK74Megasphaera_elsdenii SEQ_64 YK88 Eubacterium_rectale SEQ_65 YK89Eubacterium_rectale SEQ_66 YK97 Roseburia_faecis SEQ_67 YK98Blautia_faecis SEQ_68 YK139 Fusicatenibacter_saccharivorans SEQ_69 YK141Dorea_formicigenerans SEQ_70 YK142 Ruminococcus_faecis SEQ_71 YK152Blautia_hansenii SEQ_72 YK155 Blautia_hansenii SEQ_73 YK157Eubacterium_rectale SEQ_74 YK160 Roseburia_faecis SEQ_75 YK166Eubacterium_rectale SEQ_76 YK168 Eubacterium_rectale SEQ_77 YK169Eubacterium_rectale SEQ_78 YK171 Eubacterium_rectale SEQ_79 YK192Roseburia_faecis

TABLE F2 Composition F, strain groupings Cluster Composition F *SCFAsXIVa Eubacterium rectale 12 A, B, L Ruminococcus faecis 8 A, LRuminococcus obeum 2 A, L Blautia faecis 1 A, L Blautia hansenii 2 A, LBlautia luti 2 A, L Anaerostipes hadrus 7 B Roseburia faecis 5 A, BFusicatenibacter saccharivorans 3 A, L Dorea formicigenerans 1 A Dorealongicatena 2 A IV Flavonifractor_plautii 2 A, B Ruminococcuschampanellensis 2 A IX Acidaminococcus fermentans 1 A, B, P Megasphaeraelsdeni 4 P other Bacteroides cellulosilyticus 1 A, S BifidobacteriumBifidum L, A *Short chain fatty acid legend: A, acetate; B, Butyrate; L,lactate; P, propionate; S, succinate

In one aspect, the disclosure provides Composition G (See e.g., FIG. 19;Table G). As shown in FIG. 19, Composition G contains bacteria that havethe following 16S rDNA sequences: SEQ ID NO:27, SEQ ID NO:43, SEQ IDNO:44, SEQ ID NO:51, SEQ ID NO:55, SEQ ID NO:68, SEQ ID NO:72, SEQ IDNO:70, SEQ ID NO:24, SEQ ID NO:34, SEQ ID NO:37, SEQ ID NO:46, SEQ IDNO:76, SEQ ID NO:77, SEQ ID NO:35, SEQ ID NO:62, SEQ ID NO:26, SEQ IDNO:63, SEQ ID NO:67, SEQ ID NO:40, SEQ ID NO:38, SEQ ID NO:47, SEQ IDNO:56, SEQ ID NO:25, and SEQ ID NO: 32.

In some embodiments, the disclosure provides compositions with two ormore purified bacterial strains that have 16S rDNA sequences selectedfrom the group consisting of SEQ ID NO:27, SEQ ID NO:43, SEQ ID NO:44,SEQ ID NO:51, SEQ ID NO:55, SEQ ID NO:68, SEQ ID NO:72, SEQ ID NO:70,SEQ ID NO:24, SEQ ID NO:34, SEQ ID NO:37, SEQ ID NO:46, SEQ ID NO:76,SEQ ID NO:77, SEQ ID NO:35, SEQ ID NO:62, SEQ ID NO:26, SEQ ID NO:63,SEQ ID NO:67, SEQ ID NO:40, SEQ ID NO:38, SEQ ID NO:47, SEQ ID NO:56,SEQ ID NO:25, and SEQ ID NO: 32.

In some embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:27, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:51, SEQ ID NO:55,SEQ ID NO:68, SEQ ID NO:72, SEQ ID NO:70, SEQ ID NO:24, SEQ ID NO:34,SEQ ID NO:37, SEQ ID NO:46, SEQ ID NO:76, SEQ ID NO:77, SEQ ID NO:35,SEQ ID NO:62, SEQ ID NO:26, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:40,SEQ ID NO:38, SEQ ID NO:47, SEQ ID NO:56, SEQ ID NO:25, and SEQ ID NO:32.

The bacterial strains in Composition G are related to the followingbacteria: Acidaminococcus fermentans, Acidaminococcus intestine,Anaerostipes hadrus, Blautia faecis, Blautia hansenii, Doreaformicigenerans, Dorea longicatena, Eubacterium rectale, Flavonifractorplautii, Fusicatenibacter saccharivorans, Megasphaera elsdenii,Roseburia faecis, Ruminococcus champanellensis, Ruminococcus albus,Ruminococcus faecis, and Ruminococcus obeum.

In some embodiments, the disclosure provides compositions with two ormore bacterial strains of species selected from the group consisting ofAcidaminococcus fermentans, Acidaminococcus intestine, Anaerostipeshadrus, Blautia faecis, Blautia hansenii, Dorea formicigenerans, Dorealongicatena, Eubacterium rectale, Flavonifractor plautii,Fusicatenibacter saccharivorans, Megasphaera elsdenii, Roseburia faecis,Ruminococcus champanellensis, Ruminococcus albus, Ruminococcus faecis,and Ruminococcus obeum.

In some embodiments, the disclosure provides compositions that includetwo or more purified bacterial strains comprise 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:92, SEQ IDNO:93, SEQ ID NO:96, SEQ ID NO:97, SEQ ID NO:99, SEQ ID NO:104, SEQ IDNO:107, SEQ ID NO:111, SEQ ID NO:112, SEQ ID NO:113, SEQ ID NO:114, SEQID NO:115, SEQ ID NO:116, SEQ ID NO:117, and SEQ ID NO:119.

TABLE G Composition G SEQ_27 YK149 Acidaminococcus_fermentans/Acidaminococcus_intesti SEQ_43 YK90 Anaerostipes_hadrus SEQ_44 YK30Anaerostipes_hadrus SEQ_51 YK34 Anaerostipes_hadrus SEQ_55 YK54Anaerostipes_hadrus SEQ_68 YK98 Blautia_faecis SEQ_72 YK152Blautia_hansenii SEQ_70 YK141 Dorea_formicigenerans SEQ_24 YK96Dorea_longicatena SEQ_34 YK87 Eubacterium_rectale SEQ_37 YK163Eubacterium_rectale SEQ_46 YK12 Eubacterium_rectale SEQ_76 YK166Eubacterium_rectale SEQ_77 YK168 Eubacterium_rectale SEQ_35 YK105Flavonifractor_plautii SEQ_62 YK70 Fusicatenibacter_saccharivoransSEQ_26 YK110 Megasphaera_elsdenii SEQ_63 YK71 Roseburia_faecis SEQ_67YK97 Roseburia_faecis SEQ_40 YK99 Ruminococcus_champanellensis SEQ_38YK191 Ruminococcus_champanellensis/ Ruminococcus_albus SEQ_47 YK27Ruminococcus_faecis SEQ_56 YK56 Ruminococcus_faecis SEQ_25 YK101Ruminococcus_obeum SEQ_32 YK64 Ruminococcus_obeum

In one aspect, the disclosure provides Composition H (See e.g., FIG. 26,Table H). As shown in FIG. 26, Composition H contains bacteria that havethe following 16S rDNA sequences: SEQ ID NO:14, SEQ ID NO:16, SEQ IDNO:21, SEQ ID NO:82, SEQ ID NO:81, and SEQ ID NO:80. In someembodiments, the disclosure provides compositions with two or morepurified bacterial strains that have 16S rDNA sequences selected fromthe group consisting of SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:21, SEQ IDNO:82, SEQ ID NO:81, and SEQ ID NO:80.

In some embodiments, the compositions include two or more purifiedbacterial strains comprising 16S rDNA sequences having at least 97%homology with nucleic acid sequences selected from the group consistingof SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:21, SEQ ID NO:82, SEQ ID NO:81,and SEQ ID NO:80. In some embodiments, the compositions include four ormore purified bacterial strains comprising 16S rDNA sequences having atleast 97% homology with nucleic acid sequences selected from the groupconsisting of SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:21, SEQ ID NO:82,SEQ ID NO:81, and SEQ ID NO:80.

The bacterial strains in Composition H are related to the followingbacteria: Anaerotruncus colihominis, Clostridium symbiosum, Clostridiuminnocuum, Erysipelotrichaceae_bacterium_21-3, Clostridium disporicum,Clostridium bolteae, and Erysipelatoclostridium ramosum. In someembodiments, the disclosure provides compositions with two or morebacterial strains selected from the group consisting of Anaerotruncuscolihominis, Clostridium symbiosum, Clostridium innocuum,Erysipelotrichaceae_bacterium_21-3, Clostridium disporicum, Clostridiumbolteae, and Erysipelatoclostridium ramosum.

In some embodiments, the disclosure provides compositions that includetwo or more purified bacterial strains comprise 16S rDNA sequenceshaving at least 97% homology with nucleic acid sequences selected fromthe group consisting of SEQ ID NO:86, SEQ ID NO:95, SEQ ID NO:98, SEQ IDNO:110, SEQ ID NO:122, and SEQ ID NO:123.

Composition H includes bacteria from Clostridium cluster I, IV, XIVa,XVII and XVIII. In some embodiments, the disclosure providescompositions that include two or more purified bacterial strains fromClostridium cluster I, IV, XIVa, XVII and XVIII. In some embodiments, atleast one of the bacterial strains of the composition belongs toClostridium cluster IV. In some embodiments, at least one of thebacterial strains of the composition belongs to Clostridium clusterXIVa. In some embodiments, at least one of the bacterial strains of thecomposition belongs to Clostridium cluster XVII. In some embodiments, atleast one of the bacterial strains of the composition belongs toClostridium cluster I. In some embodiments, at least one of thebacterial strains of the composition belongs to Clostridium clusterXVIII. In some embodiments, at least one of the bacterial strains of thecomposition belongs to Clostridium cluster XIVa and at least one of thebacterial strains belongs to Clostridium cluster IV. In someembodiments, at least one of the bacterial strains of the compositionbelongs to Clostridium cluster XIVa and at least one of the bacterialstrains belongs to Clostridium cluster XVII.

TABLE H Composition H SEQ_ID NO Strain Closest species Cluster SEQ_IDNO: 14 VE202-13 Anaerotruncus colihominis Cluster IV SEQ_ID NO: 16VE202-16 Clostridium symbiosum Cluster XIVa WAL-14163 SEQ_ID NO: 21 189Clostridium innocuum Cluster XVII SEQ_ID NO: 82 PE9 Clostridiumdisporicum Cluster I SEQ_ID NO: 81 PE5 Clostridium bolteae Cluster XIVaSEQ_ID NO: 80 VE202-18 Erysipelatoclostridium Cluster XVIII ramosum

In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein the composition does not include bacteria fromClostridium cluster I. In some embodiments, the disclosure providescompositions comprising two or more bacteria, wherein the compositiondoes not include bacteria from Clostridium cluster XVIII. In someembodiments, the disclosure provides compositions comprising two or morebacteria, wherein the composition does not include bacteria fromClostridium cluster I and does not include bacteria from Clostridiumcluster XVIII.

In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein all the bacteria are anaerobic bacteria. Insome embodiments, the disclosure provides compositions comprising two ormore bacteria, wherein all the bacteria are obligate anaerobic bacteria.

In some embodiments, the disclosure provides compositions comprising twoor more bacteria (e.g., purified bacterial strains), wherein thecomposition does not include Clostridium scindens. In some embodiments,the disclosure provides compositions comprising two or more bacteria,wherein the composition does not include Flavonifractor plautii. In someembodiments, the disclosure provides compositions comprising two or morebacteria, wherein the composition does not include Parabacteroides. Insome embodiments, the disclosure provides compositions comprising two ormore bacteria, wherein the composition does not include Lactobacillus.In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein the composition does not include Colinsella.In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein the composition does not include Dialister. Insome embodiments, the disclosure provides compositions comprising two ormore bacteria, wherein the composition does not include Raoultella. Insome embodiments, the disclosure provides compositions comprising two ormore bacteria, wherein the composition does not include Streptococcus.In some embodiments, the disclosure provides compositions comprising twoor more bacteria, wherein the composition does not includeStaphylococcus. In some embodiments, the disclosure providescompositions comprising two or more bacteria, wherein the compositiondoes not include Microbacterium. In some embodiments, the disclosureprovides compositions comprising two or more bacteria, wherein thecomposition does not include Proteobacteria. In some embodiments, thedisclosure provides compositions comprising two or more bacteria,wherein the composition does not include Peptostreptococcaceae. In someembodiments, the disclosure provides compositions comprising two or morebacteria, wherein the composition does not include Oscillospiraceae.

In one aspect, the disclosure provides bacterial strains with 16S rDNAsequences that have homology to a nucleic acid sequence of any one ofthe sequences of the bacterial strains or species described herein. Insome embodiments, the bacterial strain has at least 80%, 81%, 82%, 83%,84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%,98%, 99%, 99.5%, 99.6%, 99.7%, 99.8% or 99.9% homology relative to anyof the strains or bacterial species described herein over a specifiedregion or over the entire sequence. It would be appreciated by one ofskill in the art that the term “homology” or “percent homology,” in thecontext of two or more nucleic acid sequences or amino acid sequences,refers to a measure of similarity between two or more sequences orportion(s) thereof. The homology may exist over a region of a sequencethat is at least about 50 nucleotides in length, or more preferably overa region that is 100 to 500 or 1000 or more nucleotides in length. Insome embodiments, the homology exists over the length the 16S rRNA or16S rDNA sequence, or a portion thereof.

Additionally, or alternatively, two or more sequences may be assessedfor the identity between the sequences. The terms “identical” or percent“identity” in the context of two or more nucleic acids or amino acidsequences, refer to two or more sequences or subsequences that are thesame. Two sequences are “substantially identical” if two sequences havea specified percentage of amino acid residues or nucleotides that arethe same (e.g., at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%,99.6%, 99.7%, 99.8% or 99.9% identical) over a specified region or overthe entire sequence, when compared and aligned for maximumcorrespondence over a comparison window, or designated region asmeasured using one of the following sequence comparison algorithms or bymanual alignment and visual inspection. Optionally, the identity existsover a region that is at least about 50 nucleotides in length, or morepreferably over a region that is 100 to 500 or 1000 or more nucleotidesin length. In some embodiments, the identity exists over the length the16S rRNA or 16S rDNA sequence.

Additionally, or alternatively, two or more sequences may be assessedfor the alignment between the sequences. The terms “alignment” orpercent “alignment” in the context of two or more nucleic acids or aminoacid sequences, refer to two or more sequences or subsequences that arethe same. Two sequences are “substantially aligned” if two sequenceshave a specified percentage of amino acid residues or nucleotides thatare the same (e.g., at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%,99.5%, 99.6%, 99.7%, 99.8% or 99.9% identical) over a specified regionor over the entire sequence, when compared and aligned for maximumcorrespondence over a comparison window, or designated region asmeasured using one of the following sequence comparison algorithms or bymanual alignment and visual inspection. Optionally, the alignment existsover a region that is at least about 50 nucleotides in length, or morepreferably over a region that is 100 to 500 or 1000 or more nucleotidesin length. In some embodiments, the identity exists over the length the16S rRNA or 16S rDNA sequence.

For sequence comparison, typically one sequence acts as a referencesequence, to which test sequences are compared. Methods of alignment ofsequences for comparison are well known in the art. See, e.g., by thelocal homology algorithm of Smith and Waterman (1970) Adv. Appl. Math.2:482c, by the homology alignment algorithm of Needleman and Wunsch, J.Mol. Biol. (1970) 48:443, by the search for similarity method of Pearsonand Lipman. Proc. Natl. Acad. Sci. USA (1998) 85:2444, by computerizedimplementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA inthe Wisconsin Genetics Software Package, Genetics Computer Group.Madison. Wis.), or by manual alignment and visual inspection (see. e.g.,Brent et al., Current Protocols in Molecular Biology, John Wiley & Sons,Inc. (Ringbou ed., 2003)). Two examples of algorithms that are suitablefor determining percent sequence identity and sequence similarity arethe BLAST and BLAST 2.0 algorithms, which are described in Altschul etal., Nuc. Acids Res. (1977) 25:3389-3402, and Altschul et al., J. Mol.Biol. (1990) 215:403-410, respectively.

In one aspect, the disclosure provides compositions comprising multiplepurified bacterial strains (e.g., Compositions A-J). For instance, FIGS.1, 13, 19, and 26 present several example compositions comprisingmultiple bacterial strains. In one aspect, the 16S rDNA sequences ofpurified bacterial strains of the compositions were compared to 16S rDNAsequences of known bacterial species/strains in a bacterial genomedatabase to identify the closest known related bacterial species to thebacterial strains disclosed herein (See e.g., Table 1). It should beappreciated that multiple bacterial strains of the compositionsdisclosed herein may have the same closest related bacterial species. Inone aspect, the disclosure provides compositions comprising one or morebacterial strains or species with 16S rDNA sequences that have homologyto a nucleic acid sequence of any one of the sequences provided by SEQID NOs:1-83 and 124-159. In some embodiments, the species with 16S rDNAsequences with homology to a nucleic acid sequence of any one of theclosest related species to any of the strains described herein,correspond to bacterial strains with 16S rDNA sequences provided by SEQID NOs:84-123.

In some embodiments, the compositions disclosed herein provide at leastone of the bacterial strains (e.g., purified bacterial strains)described herein. In some embodiments, the compositions that comprise atleast one bacterial strain, comprise at least one bacterial strain witha 16S rDNA sequence selected from any one of SEQ ID NOs:1-122 and124-159. In some embodiments, the compositions that comprise at leastone bacterial strain, comprise at least one bacterial strain with a 97%homology to 16S rDNA sequence selected from any one of SEQ ID NOs:1-122and 124-159.

In some embodiments, the compositions disclosed herein comprise two ormore bacterial strains. In some embodiments, the compositions describedherein comprise at least 2, at least 3, at least 4, at least 5, at least6, at least 7, at least 8, at least 9, at least 10, at least 11, atleast 12, at least 13, at least 14, at least 15, at least 16, at least17, at least 18, at least 19, or at least 20 or more bacterial strains(e.g., purified bacterial strains).

It should be appreciated the compositions and methods provided hereincan be distinguished from compositions and methods associated with thetreatment of C. difficile infection that are available. For instance, ithas been proposed that non-toxigenic C. difficile strains, i.e., strainsthat do not produce C. difficile toxins, may be used to treat C.difficile infection (See, e.g., U.S. Pat. No. 6,635,260). Thecompositions disclosed herein can be distinguished at least because thecompositions described herein do not comprise non-toxigenic strains ofC. difficile. Thus, in some embodiments, the compositions herein do notinclude comprise non-toxigenic strains of C. difficile. C. difficilebelongs to Clostridium cluster XI. In some embodiments, the compositionsherein do not include bacterial strains belonging to Clostridium clusterXI.

It is also considered in the art that bacterial strains expressing abile inducible 7α/β-dehydroxylation operon can be used in the treatmentof C. difficile (see, e.g., Buffie et al. Nature (2015) 517:205-208).The catalysis of bile acid 7a dihydroxylation is mediated by astereo-specific NAD(H)-dependent 3-oxo-Δ⁴-cholenoic acid oxidoreductaseencoded by the gene baiCD. In some embodiments, the compositionsprovided herein do not mediate bile acid 7-alpha-dehydroxylation.

In contrast to the findings in the art, in some embodiments, as shownherein, combinations of bacterial strains that do not encode baiCD (or ahomolog thereof), or encode a baiCD that comprises one or more mutationsthat result in a non-functional BaiCD protein (“baiCD-”), are moreeffective at treating C. difficile infection and/or reducing orinhibiting production of Toxin B by C. difficile than combinations ofbacterial strains that have a functional BaiCD protein (“baiCD+”). Thus,in some embodiments, the compositions of bacterial strains providedherein are baiCD− (i.e., the combination of the bacteria has noeffective baiCD+ function). In some embodiments, all of bacterialstrains in the compositions provided herein are baiCD−. In someembodiments, the majority (i.e., 50% or greater) of the bacterialstrains in the compositions are baiCD−. In some embodiments, themajority (i.e., 50% or greater) of the bacterial strains in thecompositions are baiCD- and the composition has no effective BaiCDfunction. In some embodiments, the minority (i.e., 50% or less) of thebacterial strains in the compositions are baiCD- and the composition hasno effective BaiCD function. In some embodiments, bacterial strains forthe compositions are selected based on the absence (or presence) of abaiCD gene or a predicted baiCD gene. In some embodiments, bacterialstrains may be modified (e.g., genetically engineered) to prevent orreduce expression of a baiCD gene and/or to reduce or eliminateNAD(H)-dependent 3-oxo-Δ⁴-cholenoic acid oxidoreductase activity ofBaiCD protein. The NAD(H)-dependent 3-oxo-Δ⁴-cholenoic acidoxidoreductase activity of a bacterial strain may be assessed by methodssuch as measuring the amount of 7α-dehydroxylated bile acid. In someembodiments, the compositions described herein comprise bacterialstrains without the baiCD operon (baiCD⁻) or baiCD function.

In some embodiments, the compositions described herein do not includeClostridium scindens. In some embodiments, the compositions describedherein do not include Barnesiella intestihominis. In some embodiments,the compositions described herein do not include Blautia hansenii. Insome embodiments, the compositions described herein do not includePseudoflavinofractor capillosus. In some embodiments, the compositionsdescribed herein do not include Clostridium scindens. Barnesiellaintestihominis, Blautia hansenii or Pseudoflavinofractor capillosus.

In some embodiments, the compositions provided herein do not includeColinsella aerofaciens. In some embodiments, the compositions providedherein do not include Acetovibrio ethanolgignens. In some embodiments,the compositions provided herein do not bacterial strains belonging toClostridum cluster I. In some embodiments, the compositions providedherein do not include Clostridium butyricum. In some embodiments, thecompositions provided herein do not include Clostridium disporicum. Insome embodiments, the compositions provided herein do not includestrains belonging to Clostridum cluster XI. In some embodiments, thecompositions provided herein do not include Clostridium glycolicum. Insome embodiments, the compositions provided herein do not includeFaecalibacterium prausnitzii. In some embodiments, the compositionsprovided herein do not include Turicibacter sanguinis. In someembodiments, the compositions provided herein do not include Eubacteriumrectale. In some embodiments, the compositions provided herein do notinclude Eubacterium ventriosum. In some embodiments, the compositionsprovided herein do not include Ruminococcus obeum. In some embodiments,the compositions provided herein do not include Pseudobutyrivibrio. Insome embodiments, the compositions provided herein do not includeChristensenellaceae. In some embodiments, the compositions do notcomprise gram-negative bacteria. In some embodiments, the compositionsdo not comprise E. coli. In some embodiments, the compositions do notcomprise fungi, such as Monilla species.

In some embodiments of the compositions provided herein, thecompositions do not include bacterial strains that are resistant to oneor more antibiotics. It should be appreciated that it may be desirableto have a mechanism to remove the bacterial compositions provided hereinfrom the body of the subject after administration. One such mechanism isto remove the bacterial compositions by antibiotic treatment. Thus, insome embodiments, the compositions do not include bacterial strains thatare resistant to one or more antibiotics. In some embodiments, thecompositions do not include bacterial strains that are resistant to oneor more antibiotics selected from the group consisting of penicillin,benzylpenicillin, ampicillin, sulbactam, amoxicillin, clavulanate,tazobactam, piperacillin, cefmetazole, vancomycin, imipenem, meropenem,metronidazole and clindamycin. In some embodiments, the compositions donot include bacterial strains that are resistant to vancomycin.

In some embodiments, the compositions include bacterial strains that aresusceptible to at least four antibiotics that are efficacious in humans.In some embodiments, the compositions include bacterial strains that aresusceptible to at least three antibiotics that are efficacious inhumans. In some embodiments, the compositions include bacterial strainsthat are susceptible to at least two antibiotics that are efficacious inhumans. In some embodiments, the compositions include bacterial strainsthat are susceptible to at least one antibiotic that is efficacious inhumans. In some embodiments, the compositions include only bacterialstrains that are susceptible to at least four antibiotics that areefficacious in humans. In some embodiments, the compositions includeonly bacterial strains that are susceptible to at least threeantibiotics that are efficacious in humans. In some embodiments, thecompositions include only bacterial strains that are susceptible to atleast two antibiotics that are efficacious in humans. In someembodiments, the compositions include bacterial strains that aresusceptible to at least one antibiotic that is efficacious in humans. Asused herein, an “antibiotic that is efficacious in a human” refers to anantibiotic that has been used to successfully treat bacterial infectionsin a human.

In some embodiments, the compositions described herein comprise sporeforming and non-spore forming bacterial strains. In some embodiments,the compositions described herein comprise spore forming bacterialstrains. In some embodiments, the compositions described herein compriseonly spore forming bacterial strains. In some embodiments, thecompositions described herein comprise only non-spore forming bacterialstrains. The spore-forming bacteria can be in spore form (i.e., asspores) or in vegetative form (i.e., as vegetative cells). In sporeform, bacteria are generally more resistant to environmental conditions,such as heat, acid, radiation, oxygen, chemicals, and antibiotics. Incontrast, in the vegetative state or actively growing state, bacteriaare more susceptible to such environmental conditions, compared to inthe spore form. In general, bacterial spores are able to germinate fromthe spore form into a vegetative/actively growing state, underappropriate conditions. For instance, bacteria in spore format maygerminate when thye are introduced in the intestine.

In some embodiments, at least one (e.g., 1, 2, 3, 4, 5, or more) of thebacterial strains in the composition is a spore former. In someembodiments, at least one (e.g., 1, 2, 3, 4, 5, or more) of thebacterial strains in the composition is in spore form. In someembodiments, at least one (e.g., 1, 2, 3, 4, 5, or more) of thebacterial strains in the composition is a non-spore former. In someembodiments, at least one (e.g., 1, 2, 3, 4, 5, or more) of thebacterial strains in the composition is in vegetative form (As discussedabove, spore forming bacteria can also be in vegetative form). In someembodiments, at least one (e.g., 1, 2, 3, 4, 5, or more) of thebacterial strains in the composition is in spore form and at least one(e.g., 1, 2, 3, 4, 5, or more) of the bacterial strains in thecomposition is in vegetative form. In some embodiments, at least onebacterial strain that is considered able to form spores (i.e., aspore-former) but is present in the composition in vegetative form. Insome embodiments, at least one bacterial strain that is considered ableto form spores is present in the composition both in spore form and invegetative form.

In some embodiments, the disclosure provides compositions wherein thecompositions comprise bacterial strains that are spore forming bacterialstrains. In some embodiments, the disclosure provides compositionswherein the compositions comprise bacterial strains that are non-sporeforming bacterial strains. In some embodiments, the disclosure providescompositions wherein the compositions comprise bacterial strains thatare spore forming bacterial strains and bacterial strains that arenon-spore forming bacterial strains. In some embodiments, the disclosureprovides compositions, wherein the compositions comprise a mixture ofbacterial strains wherein at least 10% of the bacterial strains arespore forming bacterial strains, at least 20% of the bacterial strainsare spore forming bacterial strains, at least 30% of the bacterialstrains are spore forming bacterial strains, at least 40% of thebacterial strains are spore forming bacterial strains, at least 50% ofthe bacterial strains are spore forming bacterial strains, at least 60%of the bacterial strains are spore forming bacterial strains, at least70% of the bacterial strains are spore forming bacterial strains, atleast 80% of the bacterial strains are spore forming bacterial strains,at least 90% of the bacterial strains are spore forming bacterialstrains bacteria up to 100% spore forming bacterial strains. Whether abacterial strain is a spore forming strain can be determined forinstance by evaluating the genome of the bacterial strain for thepresence of sporulation genes. However, it should be appreciated thatnot all bacteria that are predicted to encode spore forming genes can bemade to sporulate. In addition, whether a bacterial strain is a sporeforming strain can be determined by exposing the bacterial strain tostress conditions, e.g., heat or exposure to chemicals (e.g., ethanol orchloroform), that are known to induce sporulation.

It should be appreciated that spore forming bacteria can be in sporeform or in vegetative form. In some embodiments of the compositionsprovided herein, the spore forming bacteria are in spore form. In someembodiments of the compositions provided herein, the spore formingbacteria are in vegetative form. In some embodiments of the compositionsprovided herein, the spore forming bacteria are both present in sporeform and in vegetative form. In some embodiments, the disclosureprovides compositions, wherein the compositions comprise spore formingbacteria at least 10% of the spore forming bacteria are in spore format,at least 20% of the spore forming bacteria are in spore format, at least30% of the spore forming bacteria are in spore format, at least 40% ofthe spore forming bacteria are in spore format, at least 50% of thespore forming bacteria are in spore format, at least 60% of the sporeforming bacteria are in spore format, at least 70% of the spore formingbacteria are in spore format, at least 80% of the spore forming bacteriaare in spore format, at least 90% of the spore forming bacteria are inspore format, up to 100% in spore format.

It is envisioned that the bacterial strains of the compositions providedherein are alive and will be alive when they reach the target area(e.g., the intestines). Bacterial spores are considered to be alive inthis regards. In some embodiments, bacteria that are administered asspores may germinate in the target area (e.g., the intestines). Itshould further be appreciated that not all of the bacteria are alive andthe compositions can include a percentage (e.g., by weight) that is notalive. In addition, in some embodiments, the compositions includebacterial strains that are not alive when administered or at the timewhen the composition reaches the target area (e.g., the intestines). Itis envisioned that non-living bacteria may still be useful by providingsome nutrients and metabolites for the other bacterial strains in thecomposition.

Methods of inducing sporulation of spore-forming bacterial strains arewell known in the art (See e.g., Paredes-Sabja et al., Trends Microbial.(2011) 19(2):85-94). Generally, bacterial strains that are spore-formerscan be made to go into spore form by stressing the bacterial strains.Non-limiting examples of stresses that can induce sporulation are anincrease in temperature, change in the nutrients available and/orexposure to chemicals (e.g., ethanol or chloroform). It should be notedthat bacteria that are non-spore formers, for instance because they aremissing sporulation genes, cannot be made to sporulate by stress. Toprepare compositions in which all the bacterial strains are in the sporeform, the composition or bacterial cultures used to prepare thecomposition may be subjected to treatment to kill any bacteria not inspore form (e.g., in vegetative form), for example by exposing thecomposition to heat and are chemically breaking down the non-sporebacteria. The bacteria in spore format can subsequently be separatedfrom the non-spore bacteria for instance by filtration.

The amount of spores can be quantified using techniques know in the art.These techniques include phase contrast microscopy for enumeratingspores using a hemocytometer. In addition, the viability of spores canbe determined by plating the spores and growing the spores. Forinstance, spores can be plated in appropriate media and incubated in theanaerobic chamber for a period of time (e.g., 48-96 hrs.). Viability cansubsequently be determined by quantifying the colony forming units whichcorrespond to spores that germinated. For instance, spores can be platedon TCCFA plates (Taurocholate, cycloserine, cefoxintin, fructose agarplates), in which taurocholate helps the spores to germinate. Inaddition, spores can be quantified using the dipicolinic assay (DPAassay). DPA is an agent that allows for spore selection and is a clearindicator of endospores. When complexed with terbium, bright greenluminescence is observed.

In any of the compositions provided herein, in some embodiments, thebacterial strains are purified. In any of the compositions providedherein, in some embodiments, the bacterial strains are isolated. Any ofthe bacterial strains described herein may be isolated and/or purified,for example, from a source such as a culture or a microbiota sample(e.g., fecal matter). The bacterial strains used in the compositionsprovided herein generally are isolated from the microbiome of healthyindividuals. However, bacterial strains can also be isolated fromindividuals that are considered not to be healthy. In some embodiments,the compositions include strains originating from multiple individuals.

As used herein, the term “isolated” bacteria that have been separatedfrom one or more undesired component, such as another bacterium orbacterial strain, one or more component of a growth medium, and/or oneor more component of a sample, such as a fecal sample. In someembodiments, the bacteria are substantially isolated from a source suchthat other components of the source are not detected.

As also used herein, the term “purified” refers to a bacterial strain orcomposition comprising such that has been separated from one or morecomponents, such as contaminants. In some embodiments, the bacterialstrain is substantially free of contaminants. In some embodiments, oneor more bacterial strains of a composition may be independently purifiedfrom one or more other bacteria produced and/or present in a culture ora sample containing the bacterial strain. In some embodiments, abacterial strain is isolated or purified from a sample and then culturedunder the appropriate conditions for bacterial replication, e.g., underanaerobic culture conditions. The bacteria that is grown underappropriate conditions for bacterial replication can subsequently beisolated/purified from the culture in which it is grown.

In some embodiments, the bacterial strains of the compositions providedherein are obligate anaerobes. In some embodiments, the bacterialstrains of the compositions provided herein are facultative anaerobes.

Aspects of the present disclosure are related to methods for treating apathogenic infection in a subject by administering a therapeuticallyeffective amount of any of the compositions described herein. In someembodiments, the subject is a mammalian subject, such as a human,non-human primate, rodent, rabbit, sheep, pig, dog, cat, horse, or cow.In some embodiments, the subject is a human subject. In someembodiments, the subject is a pig.

In some embodiments, the subject is a carrier of a pathogenic organismand is suffering from the effects of the infection (e.g., diarrheacaused by C. difficile toxins). In some embodiments the subject is anasymptomatic carrier of a pathogen. In some embodiments, the subject isa carrier of C. difficile. In some embodiments the subject is anasymptomatic C. difficile carrier. In some embodiments, the subject hasexperienced recurrent or chronic pathogenic infections. In someembodiments, the subject is suffering from a first occurrence of aparticular pathogenic infection. In some embodiments, the subject hasbeen treated with antibiotics which resulted in the recurrence of thepathogenic infection. In some embodiments, the subject has been treatedwith antibiotics which resulted in a first occurrence of a pathogenicinfection. In some embodiments, the subject is to undergo a procedurethat puts the subject at a higher risk of infection. In someembodiments, the compositions provided herein are administered to asubject to lower the risk of becoming infected by a pathogen.

In some embodiments, the compositions provided herein are administeredto a subject if the subject has a dysbiosis (e.g., has as microbiomeassociated with a disease state). In some embodiments, treatment withthe compositions provided herein results in the change in the microbiomeof the subject. In some embodiments, treatment with the compositionsprovided herein removes the dysbiosis in the subject resulting in ahealthy microbiome. In some embodiments, treatment with the compositionsprovided herein removes the dysbiosis in the subject resulting inmicrobiome refractory or less susceptible to infection by a pathogen.

As used herein, the term “pathogen” in regard to a pathogenic infectionrefers to a microorganism (e.g., a bacterium) that causes a disease or adisease state in a subject. In some embodiments, the disease or diseasestate of the subject may include symptoms such as colitis, diarrhea,watery diarrhea, abdominal cramping, fever, blood or pus in the stool,nausea, dehydration, loss of appetite, chills, weight loss, and/orkidney failure. In some embodiments, the pathogenic infection may bediagnosed, for example, by detecting a pathogen (or protein or nucleicacid associated with a pathogen) in a fecal sample collected from thesubject. In some embodiments, the pathogenic infection may be diagnosed,for example, by comparing the microbiota of a fecal sample of thesubject with the microbiota in a fecal sample of a healthy subject.

In some embodiments, the pathogenic infection is C. difficile;Clostridium perfringens; Clostridium botulinum; Clostridium tributrycum;Clostridium sporogenes; Escherichia coli; Pseudomonas aeruginosa, suchas Multidrug Resistant Pseudomonas aeruginosa; Vancomycin ResistantEnterococci (VRE); Carbapenem Resistant Enterobacteriaceae (CRE);Neisseria gonorrheae; Acinetobacter; Multidrug Resistant Acinetobacter;Campylobacter; Multi-drug resistant Campylobacter; Candida;Fluconazole-resistant Candida; Extended spectrum beta-lactamese (ESBL)producing Enterobacteriaceae; Salmonella, Salmonella Typhimurium, Drugresistant non-typhoid Salmonella spp.; Drug resistant Salmonella Typhi;Drug resistant Shigella; Staphylococcus aureus, such as MethicillinResistant S. aureus or vancomycin resistant S. aureus; Drug resistantStreptococcus pneumoniae; Drug resistant Tuberculosis; ErythromycinResistant Group A Streptococcus; Clindamycin resistant Group BStreptococcus, and any combinations thereof. In some embodiments, thepathogenic infection is C. difficile. In some embodiments, the C.difficile is an antibiotic-resistant C. difficile, e.g., fluoroquinoloneresistant C. difficile. In some embodiments, the pathogenic infection isvancomycin-resistant Enterococci.

Additional non-limiting examples of pathogens responsible for pathogenicinfection that can be treated according to the methods provided hereinare Leishmania, Staphylococcus epidermis, Staphylococcus saprophyticus,Streptococcus pyogenes, Streptococcus pneumoniae, Streptococcusagalactiae, Enterococcus faecalis, Corynebacterium diptheriae, Bacillusanthracis, Listeria monocytogenes, Clostridium perfringens, Clostridiumtetanus, Clostridium botulinum, Clostridium difficile, Neisseriameningitidis, Neisseria gonorrhoeae, Escherichia coli, Salmonellatyphimurium, Salmonella cholerasuis, Salmonella enterica, Salmonellaenteriditis, Yersinia pestis, Yersinia pseudotuberculosis, Yersiniaenterocolitica, Vibrio cholerae, Campylobacter jejuni, Campylobacterfetus, Helicobacter pylori, Pseudomonas aeruginosa, Pseudomonas mallei,Haemophilus influenzae, Bordetella pertussis, Mycoplasma pneumoniae,Ureaplasma urealyticum, Legionella pneumophila, Treponema pallidum,Leptospira interrogans, Borrelia burgdorferi, Mycobacteriumtuberculosis, Mycobacterium leprae, Chlamydia psittaci, Chlamydiatrachomatis, Chlamydia pneumoniae, Rickettsia ricketsii, Rickettsiaakari, Rickettsia prowazekii, Brucella abortus, Brucella melitens,Brucella suis, and Francisella tularensis. In general, any bacteriumthat is capable of inducing a disease in a subject and/or that is notpresent in healthy individual is considered a pathogen herein. It shouldbe appreciated that a subject may carry multiple pathogens and/or havemultiple pathogenic infections.

Any of the compositions described herein may be administered to asubject in a therapeutically effective amount or a dose of atherapeutically effective amount to treat or prevent a pathogenicinfection (e.g., one or more pathogenic infections). The terms “treat”or “treatment” refer to reducing or alleviating one or more of thesymptoms associated with a pathogenic infection, reducing the amount ofbacterial toxin produced by the pathogenic infection, and/or reducingthe bacterial load of the pathogenic infection. The terms “prevent” or“prevention” encompass prophylactic administration and may reduce theincidence or likelihood of pathogenic infection or a recurrent orchronic pathogenic infection. For instance, in some embodiments,administration of the compositions provided herein result in a healthymicrobiome that is refractory to pathogenic infection, therebypreventing the pathogenic infection.

As used herein, a “therapeutically effective amount” of composition,such as a pharmaceutical composition, is any amount that results in adesired response or outcome in a subject, such as those describedherein, including but not limited to prevention of infection, an immuneresponse or an enhanced immune response to the pathogenic infection,prevention or reduction of symptoms associated with pathogenicinfection, and/or a reduction or inhibition of toxin production by thepathogenic infection. It should be appreciated that the term effectiveamount may be expressed in number of bacteria or bacterial spores to beadministered. It should further be appreciated that the bacteria canmultiply once administered. Thus, administration of even a relativelysmall amount of bacteria may have therapeutic effects.

In some embodiments, the therapeutically effective amount of any of thecompositions described herein is an amount sufficient to enhancesurvival of the subject, reduce the bacterial burden of the pathogenicinfection in the subject, and/or reduce or inhibit toxin production bythe pathogenic infection. In some embodiments, the therapeuticallyeffective amount is an amount sufficient to reduce the bacterial burdenof the pathogenic infection in a fecal sample from the subject by atleast 1.5-fold, 2-fold, 3-fold, 4-fold, 5-fold, 6-fold, 7-fold, 8-fold,9-fold, 10-fold, 20-fold, 30-fold, 40-fold, 50-fold, 100-fold,1000-fold, 10⁴-fold, 10⁵-fold or more, as compared to the bacterialburden in a subject with a pathogenic infection that has not receivedany of the compositions described herein, or as compared to a fecalsample from the same subject that was collected prior to administrationof any of the compositions.

In some embodiments, the compositions provided herein inhibit theproduction of a bacterial toxin, e.g., C. difficile Toxin B. In someembodiments, the therapeutically effective amount is an amountsufficient to reduce or inhibit the amount of bacterial toxin (e.g., C.difficile Toxin B) produced by pathogenic infection in a fecal samplefrom the subject by at least 1.5-fold, 2-fold, 3-fold, 4-fold, 5-fold,6-fold, 7-fold, 8-fold, 9-fold, 10-fold, 20-fold, 30-fold, 40-fold,50-fold, 100-fold, 150-fold, 200-fold, 500-fold or more, as compared tothe amount of the bacterial toxin in a subject with a pathogenicinfection that has not received any of the compositions described hereinor as compared to a fecal sample from the same subject that wascollected prior to administration of any of the compositions.

In some embodiments, the compositions provided herein induce theproliferation and/or accumulation of regulatory T cells in the subject.As will be evident to one of ordinary skill in the art, regulatory Tcells, also referred to as “Tregs,” are a subset of T lymphocytes thatare generally thought to suppress an abnormal or excessive immuneresponse and play a role in immune tolerance. Regulatory T cells may beidentified based expression of the markers Foxp3 and CD4 (Foxp3+CD4+).The term regulatory T cells may also include Foxp3-negative regulatory Tcells that are IL-10-producing CD4-positive T cells.

In some embodiments, the therapeutically effective amount is an amountsufficient to induce the proliferation and/or accumulation of Tregs inthe subject (or in a sample obtained from a subject) by at least1.5-fold, 2-fold, 3-fold, 4-fold, 5-fold, 6-fold, 7-fold, 8-fold,9-fold, 10-fold, 20-fold, 30-fold, 40-fold, 50-fold, 100-fold, 150-fold,200-fold, 500-fold or more, as compared to the amount of Tregs in asubject (e.g., a subject with a pathogenic infection) that has notreceived any of the compositions described herein or as compared to afecal sample from the same subject that was collected prior toadministration of any of the compositions.

As used herein, the phrase “induces proliferation and/or accumulation ofregulatory T cells” refers to an effect of inducing the differentiationof immature T cells into regulatory T cells, which differentiation leadsto the proliferation and/or the accumulation of regulatory T cells.Further, the meaning of “induces proliferation and/or accumulation ofregulatory T cells” includes in vivo effects, in vitro effects, and exvivo effects. In some embodiments, the proliferation and/or accumulationof regulatory T cells may be assessed by detecting and/or quantifyingthe number of cells that express markers of regulatory T cells (e.g.,Foxp3 and CD4), for example by flow cytometry. In some embodiments, theproliferation and/or accumulation of regulatory T cells may be assessedby determining the activity of the regulatory T cells, such as theproduction of cytokines (e.g., IL-10).

In some embodiments, the therapeutically effective amount is an amountsufficient to recolonize or repopulate the gastrointestinal tract of thesubject with non-pathogenic bacteria. In some embodiments, thetherapeutically effective amount is an amount sufficient to graft one ormore of the bacterial strains of the composition in the gastrointestinaltract of the subject. In some embodiments, a fecal sample is obtainedfrom the subject to assess the bacterial burden of the pathogenicinfection and/or evaluate the efficacy of administration of thebacterial compositions described herein. In some embodiments, themicrobiota of the subject (e.g., the identity and abundance of strainsand/or species of the microbiota) may be assessed to determine a diseasestate of the subject and/or assess progress of the treatment. In someembodiments, the microbiota of the subject having a pathogenic infectionis compared to the microbiota of a healthy subject, such as a subjectthat is not experiencing or has not experienced the pathogenicinfection. In some embodiments, the microbiota of the subject having apathogenic infection is compared to the microbiota of the same subjectfrom a fecal sample obtained from the subject prior to the pathogenicinfection.

Any of the compositions described herein, including the pharmaceuticalcompositions and food products comprising the compositions, may containbacterial strains in any form, for example in an aqueous form, such as asolution or a suspension, embedded in a semi-solid form, in a powderedform or freeze dried form. In some embodiments, the composition or thebacterial strains of the composition are lyophilized. In someembodiments, a subset of the bacterial strains in a composition islyophilized. Methods of lyophilizing compositions, specificallycompositions comprising bacteria, are well known in the art. See, e.g.,U.S. Pat. No. 3,261,761; U.S. Pat. No. 4,205,132; PCT Publications WO2014/029578 and WO 2012/098358, herein incorporated by reference intheir entirety. The bacteria may be lyophilized as a combination and/orthe bacteria may be lyophilized separately and combined prior toadministration. A bacterial strain may be combined with a pharmaceuticalexcipient prior to combining it with the other bacterial strain ormultiple lyophilized bacteria may be combined while in lyophilized formand the mixture of bacteria, once combined may be subsequently becombined with a pharmaceutical excipient. In some embodiments, thebacterial strain is a lyophilized cake. In some embodiments, thecompositions comprising the one or more bacterial strains are alyophilized cake.

The bacterial strains of the composition can be manufactured usingfermentation techniques well known in the art. In some embodiments, theactive ingredients are manufactured using anaerobic fermenters, whichcan support the rapid growth of anaerobic bacterial species. Theanaerobic fermenters may be, for example, stirred tank reactors ordisposable wave bioreactors. Culture media such as BL media and EGmedia, or similar versions of these media devoid of animal components,can be used to support the growth of the bacterial species. Thebacterial product can be purified and concentrated from the fermentationbroth by traditional techniques, such as centrifugation and filtration,and can optionally be dried and lyophilized by techniques well known inthe art.

In some embodiments, the composition of bacterial strains may beformulated for administration as a pharmaceutical composition. The term“pharmaceutical composition” as used herein means a product that resultsfrom the mixing or combining of at least one active ingredient, such asany two or more purified bacterial strains described herein, and one ormore inactive ingredients, which may include one or morepharmaceutically acceptable excipient.

An “acceptable” excipient refers to an excipient that must be compatiblewith the active ingredient and not deleterious to the subject to whichit is administered. In some embodiments, the pharmaceutically acceptableexcipient is selected based on the intended route of administration ofthe composition, for example a composition for oral or nasaladministration may comprise a different pharmaceutically acceptableexcipient than a composition for rectal administration. Examples ofexcipients include sterile water, physiological saline, solvent, a basematerial, an emulsifier, a suspending agent, a surfactant, a stabilizer,a flavoring agent, an aromatic, an excipient, a vehicle, a preservative,a binder, a diluent, a tonicity adjusting agent, a soothing agent, abulking agent, a disintegrating agent, a buffer agent, a coating agent,a lubricant, a colorant, a sweetener, a thickening agent, and asolubilizer.

Pharmaceutical compositions of the invention can be prepared inaccordance with methods well known and routinely practiced in the art(see e.g., Remington: The Science and Practice of Pharmacy, MackPublishing Co. 20th ed. 2000). The pharmaceutical compositions describedherein may further comprise any carriers or stabilizers in the form of alyophilized formulation or an aqueous solution. Acceptable excipients,carriers, or stabilizers may include, for example, buffers,antioxidants, preservatives, polymers, chelating reagents, and/orsurfactants. Pharmaceutical compositions are preferably manufacturedunder GMP conditions. The pharmaceutical compositions can be usedorally, nasally or parenterally, for instance, in the form of capsules,tablets, pills, sachets, liquids, powders, granules, fine granules,film-coated preparations, pellets, troches, sublingual preparations,chewables, buccal preparations, pastes, syrups, suspensions, elixirs,emulsions, liniments, ointments, plasters, cataplasms, transdermalabsorption systems, lotions, inhalations, aerosols, injections,suppositories, and the like.

In some embodiments, the bacteria are formulated for delivery to theintestines (e.g., the small intestine and/or the colon). In someembodiments, the bacteria are formulated with an enteric coating thatincreases the survival of the bacteria through the harsh environment inthe stomach. The enteric coating is one which resists the action ofgastric juices in the stomach so that the bacteria which areincorporated therein will pass through the stomach and into theintestines. The enteric coating may readily dissolve when in contactwith intestinal fluids, so that the bacteria enclosed in the coatingwill be released in the intestinal tract. Enteric coatings may consistof polymer and copolymers well known in the art, such as commerciallyavailable EUDRAGIT (Evonik Industries). (See e.g., Zhang, AAPSPharmSciTech, (2016) 17 (1), 56-67).

The bacteria may also be formulated for rectal delivery to the intestine(e.g., the colon). Thus, in some embodiments, the bacterial compositionsmay be formulated for delivery by suppository, colonoscopy, endoscopy,sigmoidoscopy or enema. A pharmaceutical preparation or formulation andparticularly a pharmaceutical preparation for oral administration, mayinclude an additional component that enables efficient delivery of thecompositions of the disclosure to the intestine (e.g., the colon). Avariety of pharmaceutical preparations that allow for the delivery ofthe compositions to the intestine (e.g., the colon) can be used.Examples thereof include pH sensitive compositions, more specifically,buffered sachet formulations or enteric polymers that release theircontents when the pH becomes alkaline after the enteric polymers passthrough the stomach. When a pH sensitive composition is used forformulating the pharmaceutical preparation, the pH sensitive compositionis preferably a polymer whose pH threshold of the decomposition of thecomposition is between about 6.8 and about 7.5. Such a numeric valuerange is a range in which the pH shifts toward the alkaline side at adistal portion of the stomach, and hence is a suitable range for use inthe delivery to the colon. It should further be appreciated that eachpart of the intestine (e.g., the duodenum, jejunum, ileum, cecum, colonand rectum), has different biochemical and chemical environment. Forinstance, parts of the intestines have different pHs, allowing fortargeted delivery by compositions that have a specific pH sensitivity.Thus, the compositions provided herein may be formulated for delivery tothe intestine or specific parts of the intestine (e.g., the duodenum,jejunum, ileum, cecum, colon and rectum) by providing formulations withthe appropriate pH sensitivity. (See e.g., Villena et al., Int J Pharm2015, 487 (1-2): 314-9).

Another embodiment of a pharmaceutical preparation useful for deliveryof the compositions to the intestine (e.g., the colon) is one thatensures the delivery to the colon by delaying the release of thecontents (e.g., the bacterial strains) by approximately 3 to 5 hours,which corresponds to the small intestinal transit time. In oneembodiment of a pharmaceutical preparation for delayed release, ahydrogel is used as a shell. The hydrogel is hydrated and swells uponcontact with gastrointestinal fluid, with the result that the contentsare effectively released (released predominantly in the colon). Delayedrelease dosage units include drug-containing compositions having amaterial which coats or selectively coats a drug or active ingredient tobe administered. Examples of such a selective coating material includein vivo degradable polymers, gradually hydrolyzable polymers, graduallywater-soluble polymers, and/or enzyme degradable polymers. A widevariety of coating materials for efficiently delaying the release isavailable and includes, for example, cellulose-based polymers such ashydroxypropyl cellulose, acrylic acid polymers and copolymers such asmethacrylic acid polymers and copolymers, and vinyl polymers andcopolymers such as polyvinylpyrrolidone.

Additional examples of pharmaceutical compositions that allow for thedelivery to the intestine (e.g., the colon) include bioadhesivecompositions which specifically adhere to the colonic mucosal membrane(for example, a polymer described in the specification of U.S. Pat. No.6,368,586) and compositions into which a protease inhibitor isincorporated for protecting particularly a biopharmaceutical preparationin the gastrointestinal tracts from decomposition due to an activity ofa protease.

Another example of a system enabling the delivery to the intestine(e.g., the colon) is a system of delivering a composition to the colonby pressure change in such a way that the contents are released byutilizing pressure change caused by generation of gas in bacterialfermentation at a distal portion of the stomach. Such a system is notparticularly limited, and a more specific example thereof is a capsulewhich has contents dispersed in a suppository base and which is coatedwith a hydrophobic polymer (for example, ethyl cellulose).

A further example of a system enabling the delivery of a composition tothe intestine (e.g., the colon), is a composition that includes acoating that can be removed by an enzyme present in the gut (e.g., thecolon), such as, for example, a carbohydrate hydrolase or a carbohydratereductase. Such a system is not particularly limited, and more specificexamples thereof include systems which use food components such asnon-starch polysaccharides, amylose, xanthan gum, and azopolymers.

The compositions provided herein can also be delivered to specifictarget areas, such as the intestine, by delivery through an orifice(e.g., a nasal tube) or through surgery. In addition, the compositionsprovided herein that are formulated for delivery to a specific area(e.g., the cecum or the colon), may be administered by a tube (e.g.,directly into the small intestine). Combining mechanical deliverymethods such as tubes with chemical delivery methods such as pH specificcoatings, allow for the delivery of the compositions provided herein toa desired target area (e.g., the cecum or the colon).

The compositions comprising bacterial strains are formulated intopharmaceutically acceptable dosage forms by conventional methods knownto those of skill in the art. Dosage regimens are adjusted to providethe optimum desired response (e.g., the prophylactic or therapeuticeffect). In some embodiments, the dosage form of the composition is atablet, pill, capsule, powder, granules, solution, or suppository. Insome embodiments, the pharmaceutical composition is formulated for oraladministration. In some embodiments, the pharmaceutical composition isformulated such that the bacteria of the composition, or a portionthereof, remain viable after passage through the stomach of the subject.In some embodiments, the pharmaceutical composition is formulated forrectal administration, e.g. as a suppository. In some embodiments, thepharmaceutical composition is formulated for delivery to the intestineor a specific area of the intestine (e.g., the colon) by providing anappropriate coating (e.g., a pH specific coating, a coating that can bedegraded by target area specific enzymes, or a coating that can bind toreceptors that are present in a target area).

Dosages of the active ingredients in the pharmaceutical compositions ofthe present invention can be varied so as to obtain an amount of theactive ingredient which is effective to achieve the desiredpharmaceutical response for a particular subject, composition, and modeof administration, without being toxic or having an adverse effect onthe subject. The selected dosage level depends upon a variety of factorsincluding the activity of the particular compositions of the presentinvention employed, the route of administration, the time ofadministration, the duration of the treatment, other drugs, compoundsand/or materials used in combination with the particular compositionsemployed, the age, sex, weight, condition, general health and priormedical history of the subject being treated, and like factors.

A physician, veterinarian or other trained practitioner, can start dosesof the pharmaceutical composition at levels lower than that required toachieve the desired therapeutic effect and gradually increase the dosageuntil the desired effect (e.g., treatment of a pathogenic infection,reduction of bacterial burden of pathogenic infection, reduction orinhibition of toxin production) is achieved. In general, effective dosesof the compositions of the present invention, for the prophylactictreatment of groups of people as described herein vary depending uponmany different factors, including routes of administration,physiological state of the subject, whether the subject is human or ananimal, other medications administered, and the therapeutic effectdesired. Dosages need to be titrated to optimize safety and efficacy. Insome embodiments, the dosing regimen entails oral administration of adose of any of the compositions described herein. In some embodiments,the dosing regimen entails oral administration of multiple doses of anyof the compositions described herein. In some embodiments, thecomposition is administered orally the subject once, twice, 3 times, 4times, 5 times, 6 times, 7 times, 8 times, 9 times, or at least 10times.

The compositions, including the pharmaceutical compositions disclosedherein, include compositions with a range of active ingredients (e.g.,live bacteria, bacteria in spore format). The amount of bacteria in thecompositions may be expressed in weight, number of bacteria and/or CFUs(colony forming units). In some embodiments, the pharmaceuticalcompositions disclosed herein contain about 10, about 10², about 10³,about 10⁴, about 10⁵, about 10⁶, about 10⁷, about 10⁸, about 10⁹, about10¹⁰, about 10¹¹, about 10¹², about 10¹³ or more of each of the bacteriaof the composition per dosage amount. In some embodiments, thepharmaceutical compositions disclosed herein contain about 10, about10², about 10³, about 10⁴, about 10⁵, about 10⁶, about 10⁷, about 10⁸,about 10⁹, about 10¹⁰, about 10¹¹, about 10¹², about 10¹³ or more totalbacteria per dosage amount. It should further be appreciated that thebacteria of the compositions may be present in different amounts. Thus,for instance, as a non-limiting example, a composition may include 10³of bacteria A, 10⁴ of bacteria B and 10⁶ of bacteria C. In someembodiments, the pharmaceutical compositions disclosed herein containabout 10, about 10², about 10³, about 10⁴, about 10⁵, about 10⁶, about10⁷, about 10⁸, about 10⁹, about 10¹⁰, about 10¹¹, about 10¹², about10¹³ or more CFUs of each of the bacteria in the composition per dosageamount. In some embodiments, the pharmaceutical compositions disclosedherein contain about 10¹, about 10², about 10³, about 10⁴, about 10⁵,about 10⁶, about 10⁷, about 10⁸, about 10⁹, about 10¹⁰, about 10¹¹,about 10¹², about 10¹³ or more CFUs in total for all of the bacteriacombined per dosage amount. As discussed above, bacteria of thecompositions may be present in different amounts. In some embodiments,the pharmaceutical compositions disclosed herein contain about 10⁻⁷,about 10⁻⁶, about 10⁻⁵, about 10⁴, about 10⁻³, about 10⁻², about 10⁻¹ ormore grams of each of the bacteria in the composition per dosage amount.In some embodiments, the pharmaceutical compositions disclosed hereincontain about 10⁻⁷, about 10⁻⁶, about 10⁻⁵, about 10⁴, about 10⁻³, about10⁻², about 10⁻¹ or more grams in total for all of the bacteria combinedper dosage amount. In some embodiment, the dosage amount is oneadministration device (e.g., one table, pill or capsule). In someembodiment, the dosage amount is the amount that is administered in aparticular period (e.g., one day or one week).

In some embodiments, the pharmaceutical compositions disclosed hereincontain between 10 and 10¹³, between 10² and 10¹³, between 10³ and 10¹³,between 10⁴ and 10¹³, between 10⁵ and 10¹³, between 10⁶ and 10¹³,between 10⁷ and 10¹³, between 10⁸ and 10¹³, between 10⁹ and 10¹³,between 10¹⁰ and 10¹³, between 10¹¹ and 10¹³, between 10¹² and 10¹³,between 10 and 10¹², between 10² and 10¹², between 10³ and 10¹², between10⁴ and 10¹², between 10⁵ and 10¹², between 10⁶ and 10¹², between 10⁷and 10¹², between 10⁸ and 10¹², between 10⁹ and 10¹², between 10¹⁰ and10¹², between 10¹¹ and 10¹², between 10 and 10¹¹, between 10² and 10¹¹,between 10³ and 10¹³, between 10⁴ and 10¹³, between 10⁵ and 10¹³,between 10⁶ and 10¹³, between 10⁷ and 10¹¹, between 10⁸ and 10¹¹,between 10⁹ and 10¹¹, between 10¹⁰ and 10¹¹, between 10 and 10¹⁰,between 10² and 10¹⁰, between 10³ and 10¹⁰, between 10⁴ and 10¹⁰,between 10⁵ and 10¹⁰, between 10⁶ and 10¹⁰, between 10⁷ and 10¹⁰,between 10⁸ and 10¹⁰, between 10⁹ and 10¹⁰, between 10 and 10⁹, between10² and 10⁹, between 10³ and 10⁹, between 10⁴ and 10⁹, between 10⁵ and10⁹, between 10⁶ and 10⁹, between 10⁷ and 10⁹, between 10⁸ and 10⁹,between 10 and 10⁸, between 10² and 10⁸, between 10³ and 10⁸, between10⁴ and 10⁸, between 10⁵ and 10⁸, between 10⁶ and 10⁸, between 10⁷ and10⁸, between 10 and 10⁷, between 10² and 10⁷, between 10³ and 10⁷,between 10⁴ and 10⁷, between 10⁵ and 10⁷, between 10⁶ and 10⁷, between10 and 10⁶, between 10² and 10⁶, between 10³ and 10⁶, between 10⁴ and10⁶, between 10⁵ and 10⁶, between 10 and 10⁵, between 10² and 10⁵,between 10³ and 10⁵, between 10⁴ and 10⁵, between 10 and 10⁴, between10² and 10⁴, between 10³ and 10⁴, between 10 and 10³, between 10² and10³, or between 10 and 10² of each of the bacteria of the compositionper dosage amount. In some embodiments, the pharmaceutical compositionsdisclosed herein contain between 10 and 10¹³, between 10² and 10¹³,between 10³ and 10¹³, between 10⁴ and 10¹³, between 10⁵ and 10¹³,between 10⁶ and 10¹³, between 10⁷ and 10¹³, between 10⁸ and 10¹³,between 10⁹ and 10¹³, between 10¹⁰ and 10¹³, between 10¹¹ and 10¹³,between 10¹² and 10¹³, between 10 and 10¹², between 10² and 10¹²,between 10³ and 10¹², between 10⁴ and 10¹², between 10⁵ and 10¹²,between 10⁶ and 10¹², between 10⁷ and 10¹², between 10⁸ and 10¹²,between 10⁹ and 10¹², between 10¹⁰ and 10¹², between 10¹¹ and 10¹²,between 10 and 10¹¹, between 10² and 10¹¹, between 10³ and 10¹³, between10⁴ and 10¹³, between 10⁵ and 10¹³, between 10⁶ and 10¹³, between 10⁷and 10¹¹, between 10⁸ and 10¹¹, between 10⁹ and 10¹¹, between 10¹⁰ and10¹¹, between 10 and 10¹⁰, between 10² and 10¹⁰, between 10³ and 10¹⁰,between 10⁴ and 10¹⁰, between 10⁵ and 10¹⁰, between 10⁶ and 10¹⁰,between 10⁷ and 10¹⁰, between 10⁸ and 10¹⁰, between 10⁹ and 10¹⁰,between 10 and 10⁹, between 10² and 10⁹, between 10³ and 10⁹, between10⁴ and 10⁹, between 10⁵ and 10⁹, between 10⁶ and 10⁹, between 10⁷ and10⁹, between 10⁸ and 10⁹, between 10 and 10⁸, between 10² and 10⁸,between 10³ and 10⁸, between 10⁴ and 10⁸, between 10⁵ and 10⁸, between10⁶ and 10⁸, between 10⁷ and 10⁸, between 10 and 10⁷, between 10² and10⁷, between 10³ and 10⁷, between 10⁴ and 10⁷, between 10⁵ and 10⁷,between 10⁶ and 10⁷, between 10 and 10⁶, between 10² and 10⁶, between10³ and 10⁶, between 10⁴ and 10⁶, between 10⁵ and 10⁶, between 10 and10⁵, between 10² and 10⁵, between 10³ and 10⁵, between 10⁴ and 10⁵,between 10 and 10⁴, between 10² and 10⁴, between 10³ and 10⁴, between 10and 10³, between 10² and 10³, or between 10 and 10² total bacteria perdosage amount.

In some embodiments, the pharmaceutical compositions disclosed hereincontain between 10 and 10¹³, between 10² and 10¹³, between 10³ and 10¹³,between 10⁴ and 10¹³, between 10⁵ and 10¹³, between 10⁶ and 10¹³,between 10⁷ and 10¹³, between 10⁸ and 10¹³, between 10⁹ and 10¹³,between 10¹⁰ and 10¹³, between 10¹¹ and 10¹³, between 10¹² and 10¹³,between 10 and 10¹², between 10² and 10¹², between 10³ and 10¹², between10⁴ and 10¹², between 10⁵ and 10¹², between 10⁶ and 10¹², between 10⁷and 10¹², between 10⁸ and 10¹², between 10⁹ and 10¹², between 10¹⁰ and10¹², between 10¹¹ and 10¹², between 10 and 10¹¹, between 10² and 10¹¹,between 10³ and 10¹³, between 10⁴ and 10¹³, between 10⁵ and 10¹³,between 10⁶ and 10¹³, between 10⁷ and 10¹¹, between 10⁸ and 10¹¹,between 10⁹ and 10¹¹, between 10¹⁰ and 10¹¹, between 10 and 10¹⁰,between 10² and 10¹⁰, between 10³ and 10¹⁰, between 10⁴ and 10¹⁰,between 10⁵ and 10¹⁰, between 10⁶ and 10¹⁰, between 10⁷ and 10¹⁰,between 10⁸ and 10¹⁰, between 10⁹ and 10¹⁰, between 10 and 10⁹, between10² and 10⁹, between 10³ and 10⁹, between 10⁴ and 10⁹, between 10⁵ and10⁹, between 10⁶ and 10⁹, between 10⁷ and 10⁹, between 10⁸ and 10⁹,between 10 and 10⁸, between 10² and 10⁸, between 10³ and 10⁸, between10⁴ and 10⁸, between 10⁵ and 10⁸, between 10⁶ and 10⁸, between 10⁷ and10⁸, between 10 and 10⁷, between 10² and 10⁷, between 10³ and 10⁷,between 10⁴ and 10⁷, between 10⁵ and 10⁷, between 10⁶ and 10⁷, between10 and 10⁶, between 10² and 10⁶, between 10³ and 10⁶, between 10⁴ and10⁶, between 10⁵ and 10⁶, between 10 and 10⁵, between 10² and 10⁵,between 10³ and 10⁵, between 10⁴ and 10⁵, between 10 and 10⁴, between10² and 10⁴, between 10³ and 10⁴, between 10 and 10³, between 10² and10³, or between 10 and 10² CFUs of each of the bacteria of thecomposition per dosage amount. In some embodiments, the pharmaceuticalcompositions disclosed herein contain between 10 and 10¹³, between 10²and 10¹³, between 10³ and 10¹³, between 10⁴ and 10¹³, between 10⁵ and10¹³, between 10⁶ and 10¹³, between 10⁷ and 10¹³, between 10⁸ and 10¹³,between 10⁹ and 10¹³, between 10¹⁰ and 10¹³, between 10¹¹ and 10¹³,between 10¹² and 10¹³, between 10 and 10¹², between 10² and 10¹²,between 10³ and 10¹², between 10⁴ and 10¹², between 10⁵ and 10¹²,between 10⁶ and 10¹², between 10⁷ and 10¹², between 10⁸ and 10¹²,between 10⁹ and 10¹², between 10¹⁰ and 10¹², between 10¹¹ and 10¹²,between 10 and 10¹¹, between 10² and 10¹¹, between 10³ and 10¹³, between10⁴ and 10¹³, between 10⁵ and 10¹³, between 10⁶ and 10¹³, between 10⁷and 10¹¹, between 10⁸ and 10¹¹, between 10⁹ and 10¹¹, between 10¹⁰ and10¹¹, between 10 and 10¹⁰, between 10² and 10¹⁰, between 10³ and 10¹⁰,between 10⁴ and 10¹⁰, between 10⁵ and 10¹⁰, between 10⁶ and 10¹⁰,between 10⁷ and 10¹⁰, between 10⁸ and 10¹⁰, between 10⁹ and 10¹⁰,between 10 and 10⁹, between 10² and 10⁹, between 10³ and 10⁹, between10⁴ and 10⁹, between 10⁵ and 10⁹, between 10⁶ and 10⁹, between 10⁷ and10⁹, between 10⁸ and 10⁹, between 10 and 10⁸, between 10² and 10⁸,between 10³ and 10⁸, between 10⁴ and 10⁸, between 10⁵ and 10⁸, between10⁶ and 10⁸, between 10⁷ and 10⁸, between 10 and 10⁷, between 10² and10⁷, between 10³ and 10⁷, between 10⁴ and 10⁷, between 10⁵ and 10⁷,between 10⁶ and 10⁷, between 10 and 10⁶, between 10² and 10⁶, between10³ and 10⁶, between 10⁴ and 10⁶, between 10⁵ and 10⁶, between 10 and10⁵, between 10² and 10⁵, between 10³ and 10⁵, between 10⁴ and 10⁵,between 10 and 10⁴, between 10² and 10⁴, between 10³ and 10⁴, between 10and 10³, between 10² and 10³, or between 10 and 10² total CFUs perdosage amount.

In some embodiments, the pharmaceutical compositions disclosed hereincontain between 10⁻⁷ and 10⁻¹, between 10⁻⁶ and 10⁻¹, between 10⁻⁵ and10⁻¹, between 10⁻⁴ and 10⁻¹, between 10⁻³ and 10⁻¹, between 10⁻² and10⁻¹, between 10⁻⁷ and 10⁻², between 10⁻⁶ and 10⁻², between 10⁻⁵ and10⁻², between 10⁻⁴ and 10⁻², between 10⁻³ and 10⁻², between 10⁻⁷ and10⁻³, between 10⁻⁶ and 10⁻³, between 10⁻⁵ and 10⁻³, between 10⁻⁴ and10⁻³, between 10⁻⁷ and 10⁻⁴, between 10⁻⁶ and 10⁻⁴, between 10⁻⁵ and10⁻⁴, between 10⁻⁷ and 10⁻⁵′ between 10⁻⁶ and 10⁻⁵, or between 10⁻⁷ and10⁻⁶ grams of each of the bacteria in the composition per dosage amount.In some embodiments, the pharmaceutical compositions disclosed hereincontain between 10⁻⁷ and 10⁻¹, between 10⁻⁶ and 10⁻¹, between 10⁻⁵ and10⁻¹, between 10⁻⁴ and 10⁻¹, between 10⁻³ and 10⁻¹, between 10⁻² and10⁻¹, between 10⁻⁷ and 10⁻², between 10⁻⁶ and 10⁻², between 10⁻⁵ and10⁻², between 10⁻⁴ and 10⁻², between 10⁻³ and 10⁻², between 10⁻⁷ and10⁻³, between 10⁻⁶ and 10⁻³, between 10⁻⁵ and 10⁻³, between 10⁻⁴ and10⁻³, between 10⁻⁷ and 10⁻⁴, between 10⁻⁶ and 10⁻⁴, between 10⁻⁵ and10⁻⁴, between 10⁻⁷ and 10⁻⁵′ between 10⁻⁶ and 10⁻⁵, or between 10⁻⁷ and10⁻⁶ grams of all of the bacteria combined per dosage amount.

Also with the scope of the present disclosure are food productscomprising any of the bacterial strains described herein and a nutrient.Food products are, in general, intended for the consumption of a humanor an animal. Any of the bacterial strains described herein may beformulated as a food product. In some embodiments, the bacterial strainsare formulated as a food product in spore form. In some embodiments, thebacterial strains are formulated as a food product in vegetative form.In some embodiments, the food product comprises both vegetative bacteriaand bacteria in spore form. The compositions disclosed herein can beused in a food or beverage, such as a health food or beverage, a food orbeverage for infants, a food or beverage for pregnant women, athletes,senior citizens or other specified group, a functional food, a beverage,a food or beverage for specified health use, a dietary supplement, afood or beverage for patients, or an animal feed. Non-limiting examplesof the foods and beverages include various beverages such as juices,refreshing beverages, tea beverages, drink preparations, jellybeverages, and functional beverages; alcoholic beverages such as beers;carbohydrate-containing foods such as rice food products, noodles,breads, and pastas; paste products such as fish hams, sausages, pasteproducts of seafood; retort pouch products such as curries, food dressedwith a thick starchy sauces, soups; dairy products such as milk, dairybeverages, ice creams, cheeses, and yogurts; fermented products such asfermented soybean pastes, yogurts, fermented beverages, and pickles;bean products; various confectionery products such as Westernconfectionery products including biscuits, cookies, and the like,Japanese confectionery products including steamed bean-jam buns, softadzuki-bean jellies, and the like, candies, chewing gums, gummies, colddesserts including jellies, cream caramels, and frozen desserts; instantfoods such as instant soups and instant soy-bean soups; microwavablefoods; and the like. Further, the examples also include health foods andbeverages prepared in the forms of powders, granules, tablets, capsules,liquids, pastes, and jellies.

Food products containing bacterial strains described herein may beproduced using methods known in the art and may contain the same amountof bacteria (e.g., by weight, amount or CFU) as the pharmaceuticalcompositions provided herein. Selection of an appropriate amount ofbacteria in the food product may depend on various factors, includingfor example, the serving size of the food product, the frequency ofconsumption of the food product, the specific bacterial strainscontained in the food product, the amount of water in the food product,and/or additional conditions for survival of the bacteria in the foodproduct.

Examples of food products which may be formulated to contain any of thebacterial strains described herein include, without limitation, abeverage, a drink, a bar, a snack, a dairy product, a confectioneryproduct, a cereal product, a ready-to-eat product, a nutritionalformula, such as a nutritional supplementary formulation, a food orbeverage additive.

In some embodiments, the subject has not received a dose of anantibiotic prior to administration of the bacterial composition. In someembodiments, the subject has not been administered an antibiotic atleast 1, at least 2, at least 3, at least 5, at least 10, at least 15,at least 20, at least 25, at least 30, at least 60, at least 90, atleast 120, at least 180 or at least 360 days prior to administration ofthe compositions provided herein. In some embodiments, the person hasnot been administered and antibiotic to treat the pathogenic infection.In some embodiments, the compositions provided herein comprise the firsttreatment of the pathogenic infection.

In some embodiments, the subject may be administered one or more dosesof an antibiotic prior to or concurrently with a bacterial composition.Generally, the first line of defense in the treatment of a pathogenicinfection is the administration of an antibiotic. In some embodiments,the subject is administered a single dose of an antibiotic prior to thebacterial composition. In some embodiments, the subject is administeredmultiple doses of an antibiotic prior to the bacterial composition. Insome embodiments, the subject is administered at least 2, 3, 4, 5 ormore doses of an antibiotic prior to the bacterial composition. In someembodiments, the subject is administered a dose of an antibiotic atsubstantially the same time as the bacterial composition. Examples ofantibiotics that can be administered include, without limitation,kanamycin, gentamicin, colistin, metronidazole, vancomycin, clindamycin,fidaxomicin, and cefoperazone.

Table 1 below provides sequence identifier numbers (SEQ ID NOs) used inthe compositions of the experiments disclosed herein, along with theaccompanying strain identification number (Strain ID). The closestbacterial species to the indicated strain is presented by genus-species.The 16S rDNA sequence associated with each genus species identified asthe closest related genus species is also provided. The percentalignment presents the percent identity between the sequence of theindicated strain with the sequence from the closest genus species andthe length of the alignment. The GenBank Accession Number of the closestrelated species is provided in the last column.

TABLE 1 Closest bacterial species to the strains described herein SEQ IDNO. Accession # of closest Percent Alignment of closest SEQ ID Strain IDClosest Genus_species species alignment length species SEQ ID 71 Blautiawexlerae SEQ_94 96.62 207 NR_044054 NO: 01 SEQ ID 102Turicibacter_sanguinis SEQ_91 97.81 183 NR_028816 NO: 02 SEQ ID 5Clostridium_hathewayi SEQ_105 92.42 198 NR_036928 NO: 03 SEQ ID 7Blautia_hansenii SEQ_99 96.62 207 NR_104687 NO: 04 SEQ ID 10Blautia_hansenii SEQ_99 98.06 206 NR_104687 NO: 05 SEQ ID 40Lactobacillus_mucosae SEQ_90 87.57 185 NR_024994 NO: 06 SEQ ID 59Blautia_producta SEQ_106 98.54 206 NR_113270 NO: 07 SEQ ID 59Blautia_coccoides SEQ_103 98.54 206 NR_104700 NO: 07 SEQ ID 79Blautia_hansenii SEQ_99 100 194 NR_104687 NO: 08 SEQ ID VE202-21Eubacterium_contortum SEQ_109 94.59 296 NR_117147 NO: 09 SEQ ID VE202-21Eubacterium_fissicatena SEQ_108 94.59 296 NR_117142 NO: 09 SEQ ID 211Flavonifractor_plautii SEQ_93 98.49 199 NR_043142 NO: 10 SEQ ID VE202-9Anaerostipes_caccae SEQ_88 99.5 399 NR_028915 NO: 11 SEQ ID VE202-26Clostridium_scindens SEQ_87 95.76 354 NR_028785 NO: 12 SEQ ID 136Marvinbryantia_formatexigens SEQ_89 94.66 131 NR_042152 NO: 13 SEQ IDVE202-13 Anaerotruncus_colihominis SEQ_95 99.34 1365 NR_027558 NO: 14SEQ ID VE202-14 Eubacterium_fissicatena SEQ_102 93.33 1530 NR_117563 NO:15 SEQ ID VE202-16 Clostridium_symbiosum SEQ_122 98.43 1469 NR_118730NO: 16 SEQ ID VE202-7 Clostridium_bolteae SEQ_110 99.86 1390 NR_113410NO: 17 SEQ ID 148 Dorea_longicatena SEQ_97 99.7 1318 NR_028883 NO: 18SEQ ID 16 Blautia_producta SEQ_106 98.33 1493 NR_113270 NO: 19 SEQ ID170 Dorea_longicatena SEQ_97 99.7 1318 NR_028883 NO: 20 SEQ ID 189Clostridium_innocuum SEQ_98 98.64 1476 NR_029164 NO: 21 SEQ ID 169Dorea_longicatena SEQ_97 99.58 475 NR_028883 NO: 22 SEQ ID VE202-29Eisenbergiella_tayi SEQ_121 100 354 NR_118643 NO: 23 SEQ ID YK96Dorea_longicatena SEQ_97 99.48 191 NR_028883 NO: 24 SEQ ID YK101Ruminococcus_obeum SEQ_85 96.81 188 NR_118692 NO: 25 SEQ ID YK110Megasphaera_elsdenii SEQ_119 96.62 207 NR_102980 NO: 26 SEQ ID YK149Acidaminococcus_fermentans SEQ_115 99.48 192 NR_074928 NO: 27 SEQ IDYK149 Acidaminococcus_intestini SEQ_112 99.48 192 NR_074306 NO: 27 SEQID YK154 Megasphaera_elsdenii SEQ_119 96.12 206 NR_102980 NO: 28 SEQ IDYK36 Ruminococcus_faecis SEQ_96 99.29 425 NR_116747 NO: 29 SEQ ID YK95Bacteroides_cellulosilyticus SEQ_100 99.54 437 NR_112933 NO: 30 SEQ IDYK32 Anaerostipes_hadrus SEQ_107 98.8 415 NR_104799 NO: 31 SEQ ID YK64Ruminococcus_obeum SEQ_84 99.04 415 NR_119185 NO: 32 SEQ ID YK73Flavonifractor_plautii SEQ_93 98.56 418 NR_043142 NO: 33 SEQ ID YK87Eubacterium_rectale SEQ_114 99.52 416 NR_074634 NO: 34 SEQ ID YK105Flavonifractor_plautii SEQ_93 99.26 407 NR_043142 NO: 35 SEQ ID YK153Megasphaera_elsdenii SEQ_119 96.04 429 NR_102980 NO: 36 SEQ ID YK163Eubacterium_rectale SEQ_114 99.76 415 NR_074634 NO: 37 SEQ ID YK191Ruminococcus_champanellensis SEQ_117 94.47 416 NR_102884 NO: 38 SEQ IDYK191 Ruminococcus_albus SEQ_113 94.47 416 NR_074399 NO: 38 SEQ ID YK99Ruminococcus_champanellensis SEQ_117 97.28 184 NR_102884 NO: 39 SEQ IDYK55 Ruminococcus_faecis SEQ_96 99.02 408 NR_116747 NO: 40 SEQ ID YK75Bifidobacterium_bifidum SEQ_118 99.45 183 NR_102971 NO: 41 SEQ ID YK90Anaerostipes_hadrus SEQ_107 98.97 194 NR_104799 NO: 42 SEQ ID YK30Anaerostipes_hadrus SEQ_107 99.48 191 NR_104799 NO: 43 SEQ ID YK31Anaerostipes_hadrus SEQ_107 98.97 194 NR_104799 NO: 44 SEQ ID YK12Eubacterium_rectale SEQ_114 99.27 412 NR_074634 NO: 45 SEQ ID YK27Ruminococcus_faecis SEQ_96 99.51 412 NR_116747 NO: 46 SEQ ID YK28Blautia_luti SEQ_111 99.5 400 NR_041960 NO: 47 SEQ ID YK29Ruminococcus_faecis SEQ_96 99.03 413 NR_116747 NO: 48 SEQ ID YK33Anaerostipes_hadrus SEQ_107 99.27 413 NR_104799 NO: 49 SEQ ID YK34Anaerostipes_hadrus SEQ_107 99.51 410 NR_104799 NO: 50 SEQ ID YK35Ruminococcus_faecis SEQ_96 99.51 409 NR_116747 NO: 51 SEQ ID YK51Eubacterium_rectale SEQ_114 99.27 413 NR_074634 NO: 52 SEQ ID YK52Eubacterium_rectale SEQ_114 99.03 413 NR_074634 NO: 53 SEQ ID YK54Anaerostipes_hadrus SEQ_107 85.82 409 NR_104799 NO: 54 SEQ ID YK56Ruminococcus_faecis SEQ_96 99.03 413 NR_116747 NO: 55 SEQ ID YK57Ruminococcus_faecis SEQ_96 98.79 413 NR_116747 NO: 56 SEQ ID YK58Dorea_longicatena SEQ_97 98.8 417 NR_028883 NO: 57 SEQ ID YK65Roseburia_faecis SEQ_92 99.27 413 NR_042832 NO: 58 SEQ ID YK67Blautia_luti SEQ_111 98.57 419 NR_041960 NO: 59 SEQ ID YK69Fusicatenibacter_saccharivorans SEQ_116 99.27 413 NR_114326 NO: 60 SEQID YK70 Fusicatenibacter_saccharivorans SEQ_116 98.79 414 NR_114326 NO:61 SEQ ID YK71 Roseburia_faecis SEQ_92 99.28 414 NR_042832 NO: 62 SEQ IDYK74 Megasphaera_elsdenii SEQ_119 96.06 431 NR_102980 NO: 63 SEQ ID YK88Eubacterium_rectale SEQ_114 99.28 415 NR_074634 NO: 64 SEQ ID YK89Eubacterium_rectale SEQ_114 99.27 413 NR_074634 NO: 65 SEQ ID YK97Roseburia_faecis SEQ_92 99.28 414 NR_042832 NO: 66 SEQ ID YK98Blautia_faecis SEQ_104 98.02 405 NR_109014 NO: 67 SEQ ID YK139Fusicatenibacter_saccharivorans SEQ_116 99.03 412 NR_114326 NO: 68 SEQID YK141 Dorea_formicigenerans SEQ_120 98.51 402 NR_044645 NO: 69 SEQ IDYK142 Ruminococcus_faecis SEQ_96 98.79 413 NR_116747 NO: 70 SEQ ID YK152Blautia_hansenii SEQ_99 99.5 401 NR_104687 NO: 71 SEQ ID YK155Blautia_hansenii SEQ_99 98.79 413 NR_104687 NO: 72 SEQ ID YK157Eubacterium_rectale SEQ_114 99.27 413 NR_074634 NO: 73 SEQ ID YK160Roseburia_faecis SEQ_92 99.03 414 NR_042832 NO: 74 SEQ ID YK166Eubacterium_rectale SEQ_114 99.27 409 NR_074634 NO: 75 SEQ ID YK168Eubacterium_rectale SEQ_114 99.27 413 NR_074634 NO: 76 SEQ ID YK169Eubacterium_rectale SEQ_114 99.28 416 NR_074634 NO: 77 SEQ ID YK171Eubacterium_rectale SEQ_114 97.87 188 NR_074634 NO: 78 SEQ ID YK192Roseburia_faecis SEQ_92 99.03 414 NR_042832 NO: 79 SEQ ID VE202-18Erysipelatoclostridium_ramosum SEQ_123 100 1485 NR_113243 NO: 80 SEQ IDPE5 Clostridium_bolteae SEQ_110 100 1385 NR_113410 NO: 81 SEQ ID PE9Clostridium_disporicum SEQ_86 99.21 382 NR_026491 NO: 82 SEQ ID 211-BBacteroides_ovatus SEQ_101 95.64 436 NR_112940 NO: 83

TABLE 2 Bacterial species with a high degree of homology based on wholegenome analysis: Strain Whole genome homology SEQ_10-211 Lachnospiraceaebacterium 7_1_58FAA Subdoligranulum Flavinofractor plautiiSEQ_14-VE202-13 Anaerotruncus_colihominis SEQ_15-VE202-14Eubacterium_fissicatena Ruminococcus torques SEQ_16-VE202-16Clostridium_symbiosum SEQ_17-VE202-7 Clostridium_bolteaeSEQ_22-169/SEQ_20-170 Dorea_longicatena SEQ_19-16 Blautia_productaSEQ_21-189 Clostridium_innocuum Erysipelotrichaceae_bacterium_21_3

TABLE 3 Bacterial species with highest degree of homology based on wholegenome analysis SEQ ID # Closest *Consensus Closest species of 16Sspecies SEQ SEQ based on region as based ID # of ID # Concensus SEQClosest Compo- deter- on Sanger 16S of 16S ID # of 16S species sitionStrain mined by sequencing regions as region as region as based on WGSClos- B strain iden- Sanger se- of 16S determined determined comparedwith compared versus Additional closely tridium number tifier quencingregion by WGS{circumflex over ( )} by WGS 16S database WG databasesrelated sequences cluster 1 VE202-7 17 Clostridium 124, 125, 124Clostridium Clostridium XIVa bolteae 126, 127, bolteae bolteae 90A9 1282 VE202-13 14 Anaerotruncus 129,130, 129 Anaerotruncus Anaerotruncus IVcolihominis 131 colihominis colihominis DSM 17241 3 VE202-14 15Eubacterium 132, 133, 132 Dracourtella Dracourtella Ruminococcus XIVafissicatena 134, 135, massiliensis massiliensis torques; 136 GD1Sellimonas intestinalis 4 VE202-16 16 Clostridium 137, 138, 137Clostridium Clostridium XIVa symbiosum 139, 140 symbiosum symbiosumWAL-14163 5 strain 19 Blautia 141, 142, 141 Blautia Clostridium BlautiaXIVa #16 producta 143, 144, producta bacterium UC5.1- product 145 1D4ATCC 27340 6 strain 20 Dorea 146, 147, 146 Dorea Dorea XIVa #170longicatena 148, 149, longicatena longicatena 150, 151 CAG:42 7 strain21 Clostridium 152, 153, 152 Clostridium Erysipelotrichaceae XVII #189innocuum 154, 155, innocuum bacterium 21_3 156 8 strain 10Flavinofractor 157, 158, 157 Flavinofractor ClostridiumSubdolinogranulum IV #211 plautii 159 plautii orbiscindens 1_3_50AFAA{circumflex over ( )}WGS refers to Whole Genome Sequencing performed ona PacBio Biosciences platform (Menlo Park, CA). *Consensus sequence isdefined as the 16S sequence that has the most overlap with all otheridentified 16S sequences.

In some embodiments, in any of the compositions described herein,Clostridum bolteae can be replaced with Clostridium bolteae 90A9. Insome embodiments, in any of the compositions described herein,Anaerotruncus colihominis can be replaced with Anaerotruncus colihominisDSM 17241. In some embodiments, in any of the compositions describedherein, Eubacterium fissicatena can be replaced with Sellimonasinstestinalis, Drancourtella massiliensis or Drancourtella massiliensisGP1. In some embodiments, in any of the compositions described herein,Clostridium symbiosum can be replaced with Clostridium symbiosumWAL-14163. In some embodiments, in any of the compositions describedherein, Blautia producta can be replaced with Clostridium bacteriumCD5.1-1D4 or Blautia product ATCC27340. In some embodiments, in any ofthe compositions described herein, Dorea longicatena can be replacedwith Dorea longicatena CAG:42. In some embodiments, in any of thecompositions described herein, Clostridium innocuum can be replaced withErysipelotrichaceae bacterium 21_3. In some embodiments, in any of thecompositions described herein, Flavonifractor plautii can be replacedwith Clostridium orbiscindens 1_3_50AFAA.

Aspects described herein provide pharmaceutical composition comprising apurified bacterial mixture consisting of bacterial strains comprising16S rDNA sequences of at least 95% homology to SEQ ID NO:10, SEQ IDNO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ IDNO:20, and SEQ ID NO:21. In some aspects, the bacterial strains have atleast 97% homology to SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ IDNO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21. Insome aspects, the bacterial strains have at least 98% homology to SEQ IDNO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:19, SEQ ID NO:20, and SEQ ID NO:21. In some aspects, the bacterialstrains have at least 99% homology to SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQID NO:21.

In some aspects, at least a portion of the bacteria of thepharmaceutical composition are in spore-form. In some aspects, thepharmaceutical composition further comprises a pharmaceuticallyacceptable excipient.

In some aspects, the pharmaceutical composition is formulated for oraladministration. In some aspects, the pharmaceutical composition is inthe form of a capsule. In some aspects, the pharmaceutical compositionis formulated for delivery to the colon. In some aspects, thepharmaceutical composition further comprises a pH sensitive compositioncomprising one or more enteric polymers.

Aspects described herein provide pharmaceutical compositions comprisinga purified bacterial mixture consisting of bacterial strains comprising16S rDNA sequences of at least 95% homology to SEQ ID NO:124, SEQ IDNO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQID NO:152, and SEQ ID NO:157. In some aspects, the bacterial strainshave at least 97% homology to SEQ ID NO:124, SEQ ID NO:129, SEQ IDNO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152, andSEQ ID NO:157. In some aspects, the bacterial strains have at least 98%homology to SEQ ID NO:124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137,SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152, and SEQ ID NO:157. In someaspects, the bacterial strains have at least 99% homology to SEQ IDNO:124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQID NO:146, SEQ ID NO:152, and SEQ ID NO:157.

In some aspects, at least a portion of the bacterial strains are inspore-form. In some aspects, the pharmaceutical composition furthercomprises a pharmaceutically acceptable excipient.

In some aspects, the pharmaceutical composition is formulated for oraladministration. In some aspects, the pharmaceutical composition is inthe form of a capsule. In some aspects, the pharmaceutical compositionis formulated for delivery to the colon. In some aspects, thepharmaceutical composition further comprises a pH sensitive compositioncomprising one or more enteric polymers.

Aspects described herein provide pharmaceutical compositions comprisinga purified bacterial mixture comprising bacterial strains comprising 16SrDNA sequences of at least 95% homology to SEQ ID NO:10, SEQ ID NO:14,SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20,and SEQ ID NO:21. In some aspects, the bacterial strains have at least97% homology to SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16,SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21. In someaspects, the bacterial strains have at least 98% homology to SEQ IDNO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ IDNO:19, SEQ ID NO:20, and SEQ ID NO:21. In some aspects, the bacterialstrains have at least 99% homology to SEQ ID NO:10, SEQ ID NO:14, SEQ IDNO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, and SEQID NO:21.

In some aspects, at least a portion of the bacterial strains are inspore-form. In some aspects, the pharmaceutical composition furthercomprises a pharmaceutically acceptable excipient.

In some aspects, the pharmaceutical composition is formulated for oraladministration. In some aspects, the pharmaceutical composition is inthe form of a capsule. In some aspects, the pharmaceutical compositionis formulated for delivery to the colon. In some aspects, thepharmaceutical composition further comprises a pH sensitive compositioncomprising one or more enteric polymers.

Aspects described herein provide pharmaceutical compositions comprisinga purified bacterial mixture comprising bacterial strains comprising 16SrDNA sequences of at least 95% homology to SEQ ID NO:124, SEQ ID NO:129,SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ IDNO:152, and SEQ ID NO:157. In some aspects, the bacterial strains haveat least 97% homology to SEQ ID NO:124, SEQ ID NO:129, SEQ ID NO:132,SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152, and SEQ IDNO:157. In some aspects, the bacterial strains have at least 98%homology to SEQ ID NO:124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137,SEQ ID NO:141, SEQ ID NO:146, SEQ ID NO:152, and SEQ ID NO:157. In someaspects, the bacterial strains have at least 99% homology to SEQ IDNO:124, SEQ ID NO:129, SEQ ID NO:132, SEQ ID NO:137, SEQ ID NO:141, SEQID NO:146, SEQ ID NO:152, and SEQ ID NO:157. In some aspects, at least aportion of the bacterial strains are in spore-form. In some aspects, thepharmaceutical composition further comprises a pharmaceuticallyacceptable excipient.

In some aspects, the pharmaceutical composition is formulated for oraladministration. In some aspects, the pharmaceutical composition is inthe form of a capsule. In some aspects, the pharmaceutical compositionis formulated for delivery to the colon. In some aspects, thepharmaceutical composition further comprises a pH sensitive compositioncomprising one or more enteric polymers.

Aspects described herein provide pharmaceutical compositions comprisinga purified bacterial mixture consisting of the following bacterialstrains: Clostridium bolteae, Anaerostruncus colihominis, Sellimonasintestinalis, Clostridium symbiosum, Blautia producta, DoreaLongicatena, Erysipelotrichaceae bacterium, and Clostridiumorbiscindens.

In some aspects, at least a portion of the bacterial strains are inspore-form. In some aspects, the pharmaceutical composition furthercomprises a pharmaceutically acceptable excipient.

In some aspects, the pharmaceutical composition is formulated for oraladministration. In some aspects, the pharmaceutical composition is inthe form of a capsule. In some aspects, the pharmaceutical compositionis formulated for delivery to the colon. In some aspects, thepharmaceutical composition further comprises a pH sensitive compositioncomprising one or more enteric polymers.

Aspects described herein provide pharmaceutical compositions comprisinga purified bacterial mixture comprising the following bacterial strains:Clostridium bolteae, Anaerostruncus colihominis, Sellimonasintestinalis, Clostridium symbiosum, Blautia producta, DoreaLongicatena, Erysipelotrichaceae bacterium, and Clostridiumorbiscindens.

In some aspects, at least a portion of the bacterial strains are inspore-form. In some aspects, the pharmaceutical composition furthercomprises a pharmaceutically acceptable excipient.

In some aspects, the pharmaceutical composition is formulated for oraladministration. In some aspects, the pharmaceutical composition is inthe form of a capsule. In some aspects, the pharmaceutical compositionis formulated for delivery to the colon. In some aspects, thepharmaceutical composition further comprises a pH sensitive compositioncomprising one or more enteric polymers.

Aspects described herein providemethods of treating an infectiousdisease in a subject, the method comprising administering thepharmaceutical composition of any of the aspects described herein to thesubject in an amount sufficient to treat the infectious disease. In someaspects, the infectious disease is Clostridium difficile infection.

The nucleic acid sequences of the 16S rDNA, or portion thereof, for thebacterial strains described herein are provided below:

>SEQ ID NO: 01|71|GCCCGGAGCAGTTGATGTGAAGGATGGGTCACCTGTGGACTGCATTGGAACTGTCATACTTGAGTGCCGGAGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAA >SEQ ID NO: 02|102|CTAACCGTGGAGGTCATTGGAAACTGGTCAACTTGAGTGCAGAAGAGGGAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGGCTTCCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGGGCAAACAGGATTAGATCCCCCGGTAA >SEQ ID NO: 03|5|ATGAAAGCCGGGGCTCAACCCCGGTACTGCTTTGGAAACTGTTTGACTTGAGTGCTTGAGAGGTAAGTGGAATTCCTAGTGTAGCGGGAAATGTTTAGATATTAGGAGGACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGTGGCTCGATTTGTGGGGAGCAAACAGGATTATATCCCCTGGTAA >SEQ ID NO: 04|7|CGGAAGGTCTGATGTGAAGGTTGGGGCTTACCCCGGACTGCATTGGAAACTGTTTTTCTAGAGTGCCCGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCTTTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAA >SEQ ID NO: 05|10|CGATGTCTGAGTGAAGGCTGGGGCTTACCCCAGGACTGCATTGGAACTGTTTTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAA >SEQ ID NO: 06|40|TTAACCAAGAAGTGCATTGGAACTGTCAGACTTGGGGGAAAAAAAGACAGTGCAACTCCATGTGTAGCGGTGGAATGCTCCATATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGCAACTGACGCTGAGGCTCGAATTCATGGGTAAGAAAGTATTAGTCCCTTGTAA >SEQ ID NO: 07|59|ACCCGCTTGGTCTGAGGTGAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTGTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAA >SEQ ID NO: 08|79|TAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAA >SEQ ID NO: 09|VE202-21|TTGCATTGGACACTATGTCAGCTGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGCACGTTTTCTGACGTTGAGGCTCGAAATCGTGGGGAGCAAACAAAAATAGATACCCTGGTAGTCCACGCCGTAAACGATGCATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATGCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAATAAATTGACGGA >SEQ ID NO: 10|211|CCCGTCGTAGATGTGAACTGGGGGCTCACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTGGAGAGGCAATCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTCATAA >SEQ ID NO: 11|VE202-9|ACCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAAAAGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGCATGGTAAGTCAGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTTGAAACTGTCATGCTGGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTCACTGACACTGATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGAAGTCCAT >SEQ ID NO: 12|VE202-26|ATGGGAGCGTAGATGGCGACTGGGCCATATGTGACAGCCCTGGTCTCAACCCCTTAACTGCATTTGGAACTGAGTGGCTGGAGTGTCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGGTGGCAAGGACATTCGGTGCCGCAGCAAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAAATTGACGGA >SEQ ID NO: 13|136|CGCAGCGGAGTGTATCCTAGGCTCACCTGGCTGCTTTCGAACTGGTTTTCTAGATCGTGTAGAGGGGGAGATTCCTGGTGTAGCGTGAAATGCGTAGATATCTGGAGGAACACCAGTGGCGAAGGCGGCCTCCTGGACGGCAACTGACGTTGAGGCTCGAAAGTGTGGGGAGCAAACAGGATTAGATACCCTGGTAA >SEQ ID NO: 14|VE202-13|TCAAAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTACGTTTTGAAGTTTTCGGATGGACGAATGTAAGCTTAGTGGCGGACGGGTGAGTAACACGTGAGCAACCTGCCTTTCAGAGGGGGATAACAGCCGGAAACGGCTGCTAATACCGCATGATGTTGCGGGGGCACATGCCCCTGCAACCAAAGGAGCAATCCGCTGAAAGATGGGCTCGCGTCCGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGAAGACGGTCTTCGGATTGTAAACCTCTGTCTTTGGGGAAGAAAATGACGGTACCCAAAGAGGAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTAGAATGTTAAATCCATCGGCTCAACCGGTGGCTGCGTTCTAAACTGCCGTTCTTGAGTGAAGTAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTAGGTGTGGGGGGACTGACCCCTTCCGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGGATGCATAGCCTAGAGATAGGTGAAGCCCTTCGGGGCATCCAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTATTAGTTGCTACGCAAGAGCACTCTAATGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCACTAAAACAGAGGGCGGCGACACCGCGAGGTGAAGCGAATCCCGAAAAAGTGTCTCAGTTCAGATTGCAGGCTGCAACCCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTCGGTAACACCCGAAGCCAGTAGCCTAACCGCAAGGGGGGCGCTGTCGAAGGTGGGATTGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 15|VE202-14|TACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCTGTTTTCAGAATCTTCGGAGGAAGAGGACAGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGACCGCATGGTGTAGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAAAGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAGATGACGGTACCTGAGTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATAGGCAAGTCTGGAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGCAGATCTGGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGTGTGCAAAGCACATCGGTGCCGCAGCAAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCGGATGACGGGCGAGTAATGTCGCCGTCCCTTCGGGGCATCCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCAGCATATAAGGTGGGCACTCTGGAGAGACTGCCAGGGAGAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGAGGGTGACCTGGAGCGAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGCCAGTGACCCAACCTTAGAGGAGGGAGCTGTCGAAGGCGGGACGGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 16|VE202-16|ATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTTAACGGAAGTTTTCGGATGGAAGTTGAATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGTACTGGGGGACAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTATCGCATGATACAGTGTGAAAAACTCCGGTGGTACAAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAAGTGAAAGCCCGCGGCTCAACTGCGGGACTGCTTTGGAAACTGTTTAACTGGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGACTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTTGGGGAGCAAAGCTCTTCGGTGCCGTCGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGATCCGACGGGGGAGTAACGTCCCCTTCCCTTCGGGGCGGAGAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTCTAAGTAGCCAGCGGTTCGGCCGGGAACTCTTGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATCTGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAATCTCAAAAATAACGTCTCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 17|VE202-7|ATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTAAAATGAAGTTTTCGGATGGATTTTTGATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGATAACCTGCCTCACACTGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTACCGCATGGTACGGTGTGAAAAACTCCGGTGGTGTGAGATGGATCCGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGCAAGTCTGAAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGTTTTGCTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGGGGGCAAAGCCCTTCGGTGCCGTCGCAAACGCAGTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTCTTGACCGGCGTGTAACGGCGCCTTCCCTTCGGGGCAAGAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCAGGTAAAGCTGGGCACTCTAGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGACAGTGATGTGGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGCAACGCCCGAAGTCAGTGACCCAACTCGCAAGAGAGGGAGCTGCCGAAGGCGGGGCAGGTAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 18|148|AACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTTGGAAGATTCTTCGGATGAAGACTTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACCGCTTCGTAATGGAAGCTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCAGGTTAAGCTGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAACTCGCGAGGGTAAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 19|16|ATCAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTAAGTGGATCTCTTCGGATTGAAACTTATTTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGGCTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGGCCCGTAACGGGGCCTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTTAGTAGCCAGCAGGTGAAGCTGGGCACTCTAGGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGACAGCGATGTTGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCTTACAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 20|170|AACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTTGGAAGATTCTTCGGATGATTTCCTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACCGCTTCGTAATGGAAGCTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCAGGTTAAGCTGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAACTCGCGAGGGTAAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 21|189|ATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTTTCGAGGAAGCTTGCTTCCAAAGAGACTTAGTGGCGAACGGGTGAGTAACACGTAGGTAACCTGCCCATGTGTCCGGGATAACTGCTGGAAACGGTAGCTAAAACCGGATAGGTATACAGAGCGCATGCTCAGTATATTAAAGCGCCCATCAAGGCGTGAACATGGATGGACCTGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGCGTAGCCGGCCTGAGAGGGTAAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGAGTGAAGAAGGTCTTCGGATCGTAAAGCTCTGTTGTAAGTGAAGAACGGCTCATAGAGGAAATGCTATGGGAGTGACGGTAGCTTACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCGTACTAAGTCTGTAGTAAAAGGCAATGGCTCAACCATTGTAAGCTATGGAAACTGGTATGCTGGAGTGCAGAAGAGGGCGATGGAATTCCATGTGTAGCGGTAAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGGTCGCCTGGTCTGTAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGAACTAAGTGTTGGAGGAATTCAGTGCTGCAGTTAACGCAATAAGTTCTCCGCCTGGGGAGTATGCACGCAAGTGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGGAAACAAATACCCTAGAGATAGGGGGATAATTATGGATCACACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCGCATGTTACCAGCATCAAGTTGGGGACTCATGCGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCTGGGCTACACACGTACTACAATGGCGGCCACAAAGAGCAGCGACACAGTGATGTGAAGCGAATCTCATAAAGGTCGTCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCCGTCAAACCATGGGAGTCAGTAATACCCGAAGCCGGTGGCATAACCGTAAGGAGTGAGCCGTCGAAGGTAGGACCGATGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGGGAACGTGGGGATGGATCACCTCCTTT >SEQ ID NO: 22|169|AGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAGCCC >SEQ ID NO: 23|VE202-29|CAGGCTGGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCGGTAAACGATGATTGCTAGGTGTAGGTGGGTATGGACCCATCGGTGCCGCAGCTAACGCAATAAGCAATCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCC >SEQ ID NO: 24|YK96|CCGGGGCTCACCCCGGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTA >SEQ ID NO: 25|YK101|AGGGTCAACCCCTGGACTGCATTGGAAACTGTCAGGCTGGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGATGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATAACCTGGTAAA >SEQ ID NO: 26|YK110|GGGAAGTCGGTCTTAAGTGCGGGGCTTAACCCCGTGAGGGGACCGAAACTGTGAAGCTCGAGTGTCGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAAGCGGCTTTCTGGACGACAACTGACGCTGAGGCGCGAAAGCCAGGGGAGCAAACGGGATTAGATACCCCAGTAA >SEQ ID NO: 27|YK149|TAGTCTGAGTGATGCGGGGCTTAACCCCGTATGGCGTTGGATACTGGAAGTCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGTAGCGGTGAAATGCGTAGATATTGGGAGGAACACCAGTGGCGAAGGCGCCTTTCTGGACTGTGTCTGACGCTGAGATGCGAAAGCCAGGGTAGCAAACGGGATTAGATACCACGGTA >SEQ ID NO: 28|YK154|GATAGTCGGTCTTAAGTGCGGGGCTTACCCCGTGAGGGGACCGAAACTGTGAAGCTCGAGTGTCGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAAGCGGCTTTCTGGACGACAACTGACGCTGAGGCGCGAAAGCCAGGGGAGCAAACGGGATTAGATACCACGGTAA >SEQ ID NO: 29|YK36|CGTTTGCTCCACGCTTTCGAGCCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGTACTCTAGATTGACAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCGGGTTTTCACATCAGACTTGCCACTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCATCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTTTGGA >SEQ ID NO: 30|YK95|TGTCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGAGTTCTTCGTGATATCTAAGCATTTCACCGCTACACCACGAATTCCGCCTACCTCTACTGCACTCAAGACGACCAGTATCAACTGCAATTTTACGGTTGAGCCGCAAACTTTCACAGCTGACTTAATAGTCCGCCTACGCTCCCTTTAAACCCAATAAATCCGGATAACGCTTGGATCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCGTATGGTACATACAAAAAGCCACACGTGGCTCACTTTATTCCCATATAAAAGAAGTTTACAACCCATAGGGCAGTCATCCTTCACGCTACTTGGCTGGTTCAGACTCTCGTCCATTGACCAATATTCCTCACTGCTGCCTCCCGTAGGTAGTTTGGAA >SEQ ID NO: 31|YK32|CCGTTGTCACGCTTTCGTGCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGATGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCTGCACTCCAGTCTGACAGTTTCAAAAGCAGTCCCAGAGTTAAGCCCTGGGTTTTCACTTCTGACTTGCCATACCACCTACGCACCCTTTACACCCAGTAATTCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCACTCACGCGGCGTCGCTGCATCAGGGTTCCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTGGAAGTTTGGA >SEQ ID NO: 32|YK64|GCGAATGTCACGCATTCGAGCCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGCACTCAAGACTAACAGTTTCCAATGCAGTCCAGGGGTTGAGCCCCCGCCTTTCACATCAGACTTGCCAGTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCACTATCTTCCCTGCTGATAGAAGTTTACATACCGAGATACTTCTTCCTTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTGGGAGTTTGGAA >SEQ ID NO: 33|YK73|TGCTCACGCTTTCGCGCTCAGCGTCAGTTACTGTCCAGCAATCCGCCTTCGCCACTGGTGTTCCTCCGTATATCTACGCATTTCACCGCTACACACGGAATTCCGATTGCCTCTCCAGCACTCAAGAACTACAGTTTCAAATGCAGGCTGGAGGTTGAGCCCCCAGTTTTCACATCTGACTTGCAATCCCGCCTACACGCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTATTCGTCAGGTACCGTCATTTGTTTCGTCCCTGACAAAAGAAGTTTACAACCCGAAAGCCTTCTTCCTTCACGCGGCGTTGCTGGGTCAGGCTTGCGCCCATTGCCCAATATTCCCCACTGCTGCCTCCCGTGGTAGTTTGGA >SEQ ID NO: 34|YK87|TGTCCACGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGACTCCCGTAGGAGTTTGGA >SEQ ID NO: 35|YK105|CGTTTCTCCACGCTTCGCGCTCAGCGTCAGTTACTGTCCAGCAATCCGCCTTCGCCACTGGTGTTCCTCCGTATATCTACGCATTTCACCGCTACACACGGAATTCCGATTGCCTCTCCAGCACTCAAGAACTACAGTTTCAAATGCAGGCTGGAGGTTGAGCCCCCAGTTTTCACATCTGACTTGCAATCCCGCCTACACGCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTATTCGTCAGGTACCGTCATTTGTTTCGTCCCCGACAAAAGAAGTTTACAACCCGAAAGCCTTCTTCCTTCACGCGGCGTTGCTGGGTCAGGCTTGCGCCCATTGCCCAATATTCCCCACTGCTGCCTCCCTGGGAAGTTTGG >SEQ ID NO: 36|YK153|ATGTCCTGACTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCTTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTTCCTCTCCGACACTCGAGCTTCACAGTTTCGGTCCCCTCACGGGGTTAAGCCCCGCACTTTTAAGACCGACTTGCGATGCCGCCTGCGCGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTACGGTACCGTCAGGGATAACGGGTATTGACCGCTATCCTGTTCGTCCCATATAACAGAACTTTACAACCCGAAGGCCGTCATCGTTCACGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCCACTGCTGCCTCCCTGGGAAGTTTGGA >SEQ ID NO: 37|YK163|GTTTGCTCACGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTTTGG >SEQ ID NO: 38|YK191|CGTTGCTCACGCATTCGAGCCTCAGCGTCAGTTAAGCCCAGTAAGCCGCCTTCGCCACTGATGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTACTTCACTCAAGAACCACAGTTTCAAATGCAGTTTATGGGTTAAGCCCATAGTTTTCACATCTGACTTGCGATCCCGCCTACGCTCCCTTTACACCCAGTAATTCCGGACAACGCTCGCTCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTCCTCCTCAGGTACCGTCTTTTTTCGTCCCTGAAGACAGAGGTTTACAATCCTAAAACCTTCTTCCCTCACGCGGCATCGCTGCATCAGAGTTTCCTCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTTTGGAA >SEQ ID NO: 39|YK99|TGGGCTTACCCATAAACTGCATTTGAAACTGTGGTTCTTGAGTGAAGTAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACATCAGTGGCGAAGGCGGCTTACTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCAAGTAA >SEQ ID NO: 40|YK55|GTCAGCATCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGTACTCTAGATTGACAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCGGGTTTTCACATCAGACTTGCCACTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCATCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGAGTTTGGA >SEQ ID NO: 41|YK75|TCATCGCTTACGGTGGATCTGCGCCGGGTACGGGCGGGCTGGAGTGCGGTAGGGGAGACTGGAATTCCCGGTGTAACGGTGGAATGTGTAGATATCGGGAAGAACACCGATGGCGAAGGCAGGTCTCTGGGCCGTCACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACAACGGTAA >SEQ ID NO: 42|YK90|TGAACCCAGGGCTTAACTCTGGGACTGCTTTTGAACTGTCAGACTGGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACATCAGTGGCGAAGGCGGCTTACTGGACTGAAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCATGGTAA >SEQ ID NO: 43|YK30|ACCAGGGCTTAACTCTGGGACTGCTTTTGAACTGTCAGACTGGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACATCAGTGGCGAAGGCGGCTTACTGGACTGAAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAA >SEQ ID NO: 44|YK31|GAACCCAGGGCTTAACTCTGGGACTGCTTTTGAACTGTCAGACTGGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACATCAGTGGCGAAGGCGGCTTACTGGACTGAAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCCGGTAA >SEQ ID NO: 45|YK12|GAGTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGAGTTTGGA >SEQ ID NO: 46|YK27|TGTCAGCTTTCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGTACTCTAGATTGACAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCGGGTTTTCACATCAGACTTGCCACTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCATCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTTTGGA >SEQ ID NO: 47|YK28|CACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGCACTCAAGACGGGCAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCAGCCTTTCACATCAGACTTGTCCATCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAAGTTTACATACCGAGATACTTCTTCCTTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTTTGGG >SEQ ID NO: 48|YK29|GTCAGCTTTCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGTACTCTAGATTGACAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCGGGTTTTCACATCAGACTTGCCACTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCATCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTGGGGAGTTTGGA >SEQ ID NO: 49|YK33|GATGCTCAGCTTTCGTGCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGATGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCTGCACTCCAGTCTGACAGTTTCAAAAGCAGTCCCAGAGTTAAGCCCTGGGTTTTCACTTCTGACTTGCCATACCACCTACGCACCCTTTACACCCAGTAATTCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAGATACTTCTTCACTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGAAGTTTGGA >SEQ ID NO: 50|YK34|70A_009_YK34_A1_A02GTGTCAGCTTCGTGCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGATGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCTGCACTCCAGTCTGACAGTTTCAAAAGCAGTCCCAGAGTTAAGCCCTGGGTTTTCACTTCTGACTTGCCATACCACCTACGCACCCTTTACACCCAGTAATTCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAGATACTTCTTCACTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGGAGTTTGGA >SEQ ID NO: 51|YK35|GTCAGCTTCGAGCCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGTACTCTAGATTGACAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCGGGTTTTCACATCAGACTTGCCACTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCATCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCGCGTAGGAGTTTGGA >SEQ ID NO: 52|YK51|TGTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGAGTTTGGA >SEQ ID NO: 53|YK52|TTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGGAGTTTGG >SEQ ID NO: 54|YK54|TTCGGTCTGCTTTCCCCTTCTCGCGCCTCAGTGTCAGTTTCTGTCTAGTAAGCCGCCTTCGCCACTGATGTTCCTCCTAATATCTACGCACTTCACCGCTCCACAATGAATTCCGCTTACCCCTCCCGCGCTCTAGTCTGACAGTTTTAAAAAAACTCCCCGAGAGAAACCCTGGGTTTTTTCTTCTGACATGCGATATCCCACCCCCACCCTTTATACACCCAAAAATCGGATAAAAGGTGCGACCTACGTATTATACCGGCTGCTGGGGCGTAGATAGCCGGGGGTTCTTATACAGGGACCGTCATTTTCTTTCCCGCTGATACAGCTTTACATACCGAAATACTTCTTTCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGGAAGTTGGGGGAAA >SEQ ID NO: 55|YK56|GTTCAGCTTTCGAGCCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGTACTCTAGATTGACAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCGGGTTTTCACATCAGACTTGCCACTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCATCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGGAGTTTGGA >SEQ ID NO: 56|YK57|GTCAGCTTTCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGTACTCTAGATTGACAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCGGGTTTTCACATCAGACTTGCCACTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCATCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGGAGTTTGGA >SEQ ID NO: 57|YK58|TCTCACGCTTTCGAGCTCACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCACTTGCCTCTCCGACACTCTAGCTCAGCAGTTCCAAATGCAGTCCCGGGGTTGAGCCCCGGGCTTTCACATCTGGCTTGCCGTGCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAAGTTTACATACCGAAATACTTCATCCTTCACGCGGCGTCGCTGCATCAGAGTTTCCTCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGGAGTTTGG >SEQ ID NO: 58|YK65|GTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCACTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTCTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGAGTTTGGA >SEQ ID NO: 59|YK67|AGCCCCGCTTTCGAGCCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGCACTCAAGACGGGCAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCAGCCTTTCACATCAGACTTGTCCATCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAAGTTTACATACCGAGATACTTCTTCCTTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGAAGTTTGGA >SEQ ID NO: 60|YK69|TGCTCAGCTTTCGAGCCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCTTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGCACTCGAGCCAGACAGTTTCCAATGCAGTCCCAGGGTTAAGCCCTGGGTTTTCACATCAGACTTGCCTTGCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGAGTTTGGA >SEQ ID NO: 61|YK70|GTTGCTCAGCTTTCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCTTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGCACTCGAGCCAGACAGTTTCCAATGCAGTCCCAGGGTTAAGCCCTGGGTTTTCACATCAGACTTGCCTTGCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGAAAGTTTGGA >SEQ ID NO: 62|YK71|TGCTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCACTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTCTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGAGTTTGGA >SEQ ID NO: 63|YK74|GATGCCCTGGCTTCGCGCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCTTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTTCCTCTCCGACACTCGAGCTTCACAGTTTCGGTCCCCTCACGGGGTTAAGCCCCGCACTTTTAAGACCGACTTGCGATGCCGCCTGCGCGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTACGGTACCGTCAGGGATAACGGGTATTGACCGCTATCCTGTTCGTCCCATATAACAGAACTTTACAACCCGAAGGCCGTCATCGTTCACGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCCACTGCTGCCTCCCGGGGGGAGTTTGGA >SEQ ID NO: 64|YK88|GTCCCGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGAAGTTTGG >SEQ ID NO: 65|YK89|TGTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGAGTTTGGA >SEQ ID NO: 66|YK97|TGCTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCACTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTCTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGAGTTTGGA >SEQ ID NO: 67|YK98|ATTCAGCTTTCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGGCACTCAAGCATACCAGTTTCCAATGCAGTCCAGGGGTTAAGCCCCTGCCTTTCACATCAGACTTGATACGCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTCGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAAGTTTACATACCGAGATACTTCTTCCTTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGAAGTTTGGA >SEQ ID NO: 68|YK139|GTGTCAGCTTTCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCTTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGCACTCGAGCCAGACAGTTTCCAATGCAGTCCCAGGGTTAAGCCCTGGGTTTTCACATCAGACTTGCCTTGCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGAGTTTGG >SEQ ID NO: 69|YK141|GCCAGCTTCGAGCCTCACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCACTTACCTCTCCGACACTCTAGCTGCACAGTTTCCAAAGCAGTCCACAGGTTGAGCCCATGCCTTTCACTTCAGACTTGCACAGCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAAGTTTACATACCGAAATACTTCATCCTTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGAAGTTTGGA >SEQ ID NO: 70|YK142|TGATCAGCTTTCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGTACTCTAGATTGACAGTTTCCAATGCAGTCCCGGGGTTGAGCCCCGGGTTTTCACATCAGACTTGCCACTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCATCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGGGGGGAGTTTGGA >SEQ ID NO: 71|YK152|GATGATCAGCTTTCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGCACTCTAGAAAAACAGTTTCCAATGCAGTCCTGGGGTTAAGCCCCAGCCTTTCACATCAGACTTGCTCTTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAAGTTTACATACCGAGATACTTCTTCCTTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGGGGGAAGTTTGGA >SEQ ID NO: 72|YK155|TTGATCAGCTTTCGAGCTCACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTCCGGCACTCTAGAAAAACAGTTTCCAATGCAGTCCTGGGGTTAAGCCCCAGCCTTTCACATCAGACTTGCTCTTCCGTCTACGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTCTTCCCTGCTGATAGAAGTTTACATACCGAGATACTTCTTCCTTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGAGTTTGG >SEQ ID NO: 73|YK157|GTATTTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGAGTTTGGA >SEQ ID NO: 74|YK160|GCTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCACTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTCTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGGAGTTTGGA >SEQ ID NO: 75|YK166|TTTCAGCTTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTAGCTCCCGAAGGAGTTTGGA >SEQ ID NO: 76|YK168|AGCTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAAGGGAGTTTGGA >SEQ ID NO: 77|YK169|GTCCAGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTATCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGAGTTTGGA >SEQ ID NO: 78|YK171|TGAGCCGGGCTCACCCCGGTACTGCATTGGAACTGTCGTACTAGAGTGTCGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACACCGGTAA >SEQ ID NO: 79|YK192|CACGATGTCAGCTTTCGAGCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGGAATTCCACTTACCCCTCCGACACTCTAGTACGACAGTTTCCAATGCAGTACCGGGGTTGAGCCCCGGGCTTTCACATCAGACTTGCCGCACCGCCTGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCACCATACGTATTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACCGTCATTCTTCTTCCCTGCTGATAGAGCTTTACATACCGAAATACTTCTTCGCTCACGCGGCGTCGCTGCATCAGGGTTTCCCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGAGGGGAGTTTGGA >SEQ ID NO: 80|VE202-18|ATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCACTTGTGCTCGAGTGGCGAACGGGTGAGTAATACATAAGTAACCTGCCCTAGACAGGGGGATAACTATTGGAAACGATAGCTAAGACCGCATAGGTACGGACACTGCATGGTGACCGTATTAAAAGTGCCTCAAAGCACTGGTAGAGGATGGACTTATGGCGCATTAGCTGGTTGGCGGGGTAACGGCCCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGAAGGAAGAAGGTTTTCGGATTGTAAACTTCTGTTATAAAGGAAGAACGGCGGCTACAGGAAATGGTAGCCGAGTGACGGTACTTTATTAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCAGCAAGGGTCTGTGGTGAAAGCCTGAAGCTTAACTTCAGTAAGCCATAGAAACCAGGCAGCTAGAGTGCAGGAGAGGATCGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGACGATCTGGCCTGCAACTGACGCTCAGTCCCGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGTACTAAGTGTTGGATGTCAAAGTTCAGTGCTGCAGTTAACGCAATAAGTACTCCGCCTGAGTAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATACTCATAAAGGCTCCAGAGATGGAGAGATAGCTATATGAGATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACCATCATTAAGTTGGGGACTCTAGCGAGACTGCCAGTGACAAGCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTGCAGAGGGAAGCGAAGCCGCGAGGTGAAGCAAAACCCATAAAACCATTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTTGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTGATAACACCCGAAGCCGGTGGCCTAACCGCAAGGAAGGAGCTGTCTAAGGTGGGATTGATGATTGGGGTGAAGTCGTAACAAGGTATCCCTACGGGAACGTGGGGATGGATCACCTCCTTT >SEQ ID NO: 81|PE5|ATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTGAAGGAAGTTTTCGGATGGAATTCGATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGATAACCTGCCTCACACTGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTACCGCATGGTACAGTGTGAAAAACTCCGGTGGTGTGAGATGGATCCGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGCAAGTCTGAAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGTTTTGCTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGGGGGCAAAGCCCTTCGGTGCCGTCGCAAACGCAGTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTCTTGACCGGCGTGTAACGGCGCCTTCCCTTCGGGGCAAGAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCAGGTAAAGCTGGGCACTCTAGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGACAGTGATGTGGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGCAACGCCCGAAGTCAGTGACCCAACTCGCAAGAGAGGGAGCTGCCGAAGGCGGGGCAGGTAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 82|PE9|AATTCGACGTTGTCCGGATTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGATGTGAAATACCCGGGCTCAACTTGGGTGCTGCATTTCAAACTGGAAGTCTAGAGTGCAGGAGAGGAGAATGGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAACACCAGTGGCGAAGGCGATTCTCTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTAGGGGTTGTCATGACCTCTGTGCCGCCGCTAACGCATTAAGTATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGAAATTGACGGA >SEQ ID NO: 83|211-B|ACGAGCGTATCGGATTATTGGGTTTAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCGATTGCGAAGGCAGCTCACTAGACTGTCACTGACACTGATGCTCGAAAGTGTGGGTATCAAACAGGATTAGATACCCTGGTAGTCCACACAGTAAACGATGAATACTCGCTGTTTGCGATATACAGTAAGCGGCCAAGCGAAAGCATTAAGTATTCCACCTGGGGAGTACGCCGGCAACGGTGAAACTCAAAGAAATTGACGGAAGCCCGCCCAGGGGGGAAAAACATGGGGTTTAGTTGGATGATACGGGGAGGAACCTC>SEQ ID NO: 84|NR_119185.1|Ruminococcus obeum 16S ribosomal RNA gene,complete sequenceGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTTTCATTGAAGCTTCGGCAGATTTGGNNTGTTTCTAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTATACAGGGGGATAACAACCAGAAATGGTTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATAAGATGGACCCGCGTTGGATTAGCTAGTTGGCAGGGTAACGGCCTACCAAGGCGACGATCCATAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGATAGTGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGATGTGAAAGGCGGGGGCTCAACCCCTGGACTGCATTGGAAACTGTTAGTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTAGGTGTTGGGGAGCAAAGCTCTTCGGTGCCGCCGCAAACGCATTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGNCCCTTAACCGGATCTTTCCTTCGGGACAGGGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCCCAGTAGCCAGCAGTCCGGCTGGGCACTCTGAGGAGACTGCCAGGGATAACCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGCCTGCGAAGGTAAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCTAACTGCAAAGAAGGAGCTGCCGAAGGCGGGACCGATGACTGGGGTGAAGTCGTAACAAGGT>SEQ ID NO: 85|NR_118692.1|Ruminococcus obeum strain ATCC 29174 16S ribosomalRNA gene, complete sequenceGGCGTGCTTAACACATGCAAGTCGAACGGGAAACTTTTCATTGAAGCTTCGGCAGATTTGGTCTGTTTCTAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTATACAGGGGGATAACAACCAGAAATGGTTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATAAGATGGACCCGCGTTGGATTAGCTAGTTGGCAGGGTAACGGCCTACCAAGGCGACGATCCATAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGCCCCAGACTCCTCGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGATAGTGACGGTACCTGACTAAGAAGCCCCGKCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGATGTGAAAGGCGGGGGCTCAACCCCTGGACTGCATTGGAAACTGTTAGTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGCAAACGATGAATACTAGGTGTTGGGGAGCAAAGCTCTTCGGTGCCGCCGCAAACGCATTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGTCCCTTAACCGGATCTTTCCTTCGGGACAGGGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCCCAGTAGCCAGCAGTNCGGCTGGGCACTCTGAGGAGACTGCCAGGGATAACCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCNAGCCTKCGRAGGTAAGCAAATCCCANAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCTAACTGC>SEQ ID NO: 86|NR_026491.1|Clostridium disporicum strain DS1 16S ribosomalRNA gene, partial sequenceGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTTGATTCTCTTCGGAGATGAAGCTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTCATAGAGGGGAATAGCCTCCCGAAAGGGAGATTAATACCGCATAAGATTGTAGCTTCGCATGAAGTAGCAATTAAAGGAGCAATCCGCTATGAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGAGTGATGACGGCCTTCGGGTTGTAAAGCTCTGTCTTCAGGGACGATAATGACGGTACCTGAGGAGGAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGATGTGAAATACCCGGGCTCAACTTGGGTGCTGCATTTCAAACTGGAAGTCTAGAGTGCAGGAGAGGAGAATGGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAACACCAGTGGCGAAGGCGATTCTCTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTAGGGGTTGTCATGACCTCTGTGCCGCCGCTAACGCATTAAGTATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTAGACTTGACATCTCCTGAATTACCCGTAACTGGGGAAGCCACTTCGGTGGCAGGAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTGTTAGTTGCTACCATTTAGTTGAGCACTCTAGCGAGACTGCCCGGGTTAACCGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTACACACGTGCTACAATGGCAAGTACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAAAAACTTGTCTCAGTTCGGATTGTAGGCTGAAACTCGCCTACATGAAGCTGGAGTTGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTTGGCAATACCCAACGTACGTGATCTAACCCGCAAGGGAGGAAGCGTCCTAAGGTAGGGTCAGCGATTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGAGAA>SEQ ID NO: 87|NR_028785.1|Clostridium scindens strain ATCC 35704 16Sribosomal RNA gene, complete sequenceGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGCCTGGCCCCGACTTCTTCGGAACGAGGAGCCTTGCGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCTTGCACTGGGGGATAACAGCCAGAAATGGCTGCTAATACCGCATAAGACCGAAGCGCCGCATGGCGCGGCGGCCAAAGCCCCGGCGGTGCAAGATGGGCCCGCGTCTGATTAGGTAGTTGGCGGGGTAACGGCCCACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAGATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGATGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGCGTGGCTGGAGTGTCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGGTGGCAAGGCCATTCGGTGCCGCAGCAAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGCCAAAGCGCGTAACGCGCTCTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCATTTTGGATGGGCACTCTGGAGAGACTGCCAGGGAGAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCGAACCCGCGAGGGTGGGCAAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGCCGGTGACCCAACCCGTAAGGGAGGGAGCCGTCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTC>SEQ ID NO: 88|NR_028915.1|Anaerostipes caccae strain L1-92 16S ribosomalRNA gene, partial sequenceGCGCTTAATACATGTCAAGTCGAACGAAGCATTTAGGATTGAAGTTTTCGGATGGATTTCCTATATGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGGAACCTGCCCTATACAGGGGGATAACAGCTGGAAACGGCTGCTAATACCGCATAAGCGCACAGAATCGCATGATTCAGTGTGAAAAGCCCTGGCAGTATAGGATGGTCCCGCGTCTGATTAGCTGGTTGGTGAGGTAACGGCTCACCAAGGCGACGATCAGTAGCCGGCTTGAGAGAGTGAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGTAAACCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAACAGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGCATGGTAAGTCAGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTTGAAACTGTCATGCTGGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTCACTGACACTGATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGGCCGTAGAGGCTTCGGTGCCGCAGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCCAATGACCGAACCTTAACCGGTTTTTTCTTTCGAGACATTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTTAGTAGCCAGCATTTAAGGTGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGGCCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAAGTCGTGAGGCGAAGCAAATCCCAGAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGTGAATCAGAATGTCACGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGG>SEQ ID NO: 89|NR_042152.1|Marvinbryantia formatexigens strain I-52 16Sribosomal RNA gene, partial sequence >gi|636558750|ref|NR_114807.1|Marvinbryantia formatexigens strain I-52 16S ribosomal RNA gene, completesequenceTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCATTTTAAATGAAGTTTTCGGACGGAATTTAAAATGACTGAGCGGCGGACGGGTGAGTAACGCGTGGATAACCTGCCTTATACAGGGGGATAACAGCCAGAAATGGCTGCTAATACCGCATAAGCGCACGGTACCGCATGGTACAGTGTGAAAAACTCCGGTGGTATAAGATGGGTCCGCGTTGGATTAGGCAGTTGGCGGGGTAAAGGCCCACCAAACCGACGATCCATAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGGGTGAAGAAGTATTTCGGTATGTAAAGCCCTATCAGCAGGGAAGAAAATGACGGTACCTGACCAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCCATGCAAGTCTGGTGTGAAAGGCGGGGGCTCAACCCCCGGACTGCATTGGAAACTGTATGGCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACCAGGTGTCGGGGGACACGGTCCTTCGGTGCCGCAGCAAACGCACTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCGGACGACCGGACAGTAACGTGTCCTTCCCTTCGGGGCGTCCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGTTCCCAGTAGCCAGCATTCAGGATGGGCACTCTGGGGAGACTGCCAGGGATAACCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGATCTGGGCTACACACGTGCTACAATGGCGTGAACAGAGGGAAGCGAACCCGCGAGGGGGAGCAAATCCCAGAAATAACGTCCCAGTTCGGATTGTAGTCTGCAACCCGGCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCGGAAATGCCCGAAGTCAGTGACCCAACCGGAAGGAGGGAGCTGCCGAAGGCGGGGCCGGTAACTGGGGTGAAGTCGTAACAA>SEQ ID NO: 90|NR_024994.1|Lactobacillus mucosae strain S32 16S ribosomalRNA gene, complete sequenceAGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATGCAAGTCGAACGCGTTGGCCCAACTGATTGAACGTGCTTGCACGGACTTGACGTTGGTTTACCAGCGAGTGGCGGACGGGTGAGTAACACGTAGGTAACCTGCCCCAAAGCGGGGGATAACATTTGGAAACAGATGCTAATACCGCATAACAATTTGAATCGCATGATTCAAATTTAAAAGATGGCTTCGGCTATCACTTTGGGATGGACCTGCGGCGCATTAGCTTGTTGGTAGGGTAACGGCCTACCAAGGCTGTGATGCGTAGCCGAGTTGAGAGACTGATCGGCCACAATGGAACTGAGACACGGTCCATACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGATGGAGCAACACCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAGCTCTGTTGTTAGAGAAGAACGTGCGTGAGAGCAACTGTTCACGCAGTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGATGTGAAAGCCTTTGGCTTAACCAAAGAAGTGCATCGGAAACTGTCAGACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGCTAGGTGTTGGAGGGTTTCCGCCCTTCAGTGCCGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATCTTGCGCCAACCCTAGAGATAGGGCGTTTCCTTCGGGAACGCAATGACAGGTGGTGCATGGTCGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTACTAGTTGCCAGCATTCAGTTGGGCACTCTAGTGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAGATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAACGAGTCGCGAACTCGCGAGGGCAAGCTAATCTCTTAAAACCGTTCTCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTTTGCAACACCCAAAGTCGGTGGGGTAACCCTTCGGGGAGCTAGCCGCCTAAGGTGGGGCAGATGATTAGGGTGAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATCACCTCCT>SEQ ID NO: 91|NR_028816.1|Turicibacter sanguinis strain MOL361 16S ribosomalRNA gene, complete sequenceAGAGTTTGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACCACTTCGGTGGTGAGCGGCGAACGGGTGAGTAACACGTAGGTTATCTGCCCATCAGACGGGGACAACGATTGGAAACGATCGCTAATACCGGATAGGACGAAAGTTTAAAGGTGCTTCGGCACCACTGATGGATGAGCCTGCGGCGCATTAGCTAGTTGGTAGGGTAAAGGCCTACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGAACGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGACCGAGCAACGCCGCGTGAATGATGAAGGCCTTCGGGTTGTAAAATTCTGTTATAAGGGAAGAATGGCTCTAGTAGGAAATGGCTAGAGTGTGACGGTACCTTATGAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCAGGTGGTTGATTAAGTCTGATGTGAAAGCCCACGGCTTAACCGTGGAGGGTCATTGGAAACTGGTCAACTTGAGTGCAGAAGAGGGAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGGCTTCCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTGGGGGTCGAACCTCAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATACCAGTGACCGTCCTAGAGATAGGATTTTCCCTTCGGGGACAATGGATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGTCGTTAGTTGCCAGCATTCAGTTGGGGACTCTAACGAGACTGCCAGTGACAAACTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGTTGGTACAAAGAGAAGCGAAGCGGTGACGTGGAGCAAACCTCATAAAGCCAATCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGTTGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTTACAACACCCGAAGTCAGTGGCCTAACCGCAAGGAGGGAGCTGCCTAAGGTGGGGTAGATGATTGGGGTGAAGTCGTAACAAGGTATCCCTACCGGAAGGTGGGGTTGGATCACCTCCTT>SEQ ID NO: 92|NR_042832.1|Roseburia faecis strain M72/1 16S ribosomal RNAgene, partial sequenceGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCTATTTGATTTTCTTCGGAAATGAAGATTTTGTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTGGAAACGACTGCTAATACCGCATAAGCGCACAGGATCGCATGATCCGGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCCAGTTGGCAGGGTAACGGCCTACCAAAGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGCGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAGAATGACGGTACCTGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGGAGCATTGCTCTTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCGATGACAGAGTATGTAATGTACYTTCTCTTCGGAGCATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGTCCTTAGTAGCCAGCGGTTCGGCCGGGCACTCTAGGGAGACTGCCAGGGATAACCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGACTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGGAGCCGTGAGGCCGAGCAAATCTCAAAAATAACGTCTCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGAAATGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGCAGGTTCGATAACTGGGGTG>SEQ ID NO: 93|NR_043142.1|Flavonifractor plautii strain Prevot S1 16Sribosomal RNA gene, partial sequenceCGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCATGACGGAGGATTCGTCCAATGGATTGAGTTACCTAGTGGCGGACGGGTGAGTAACGCGTGAGGAACCTGCCTTGGAGAGGGGAATAACACTCCGAAAGGAGTGCTAATACCGCATGAAGCAGTTGGGTCGCATGGCTCTGACTGCCAAAGATTTATCGCTCTGAGATGGCCTCGCGTCTGATTAGCTAGTAGGCGGGGTAACGGCCCACCTAGGCGACGATCAGTAGCCGGACTGAGAGGTTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGAAGGAAGAAGGCTTTCGGGTTGTAAACTTCTTTTGTCGGGGACGAAACAAATGACGGTACCCGACGAATAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTGGAGAGGCAATCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATACTAGGTGTGGGGGGTCTGACCCCCTCCGTGCCGCAGTTAACACAATAAGTATCCCACCTGGGGAGTACGATCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGGCTTGACATCCCACTAACGAGGCAGAGATGCGTTAGGTGCCCTTCGGGGAAAGTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTGTTAGTTGCTACGCAAGAGCACTCTAGCGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGTCCTGGGCCACACACGTACTACAATGGTGGTTAACAGAGGGAGGCAATACCGCGAGGTGGAGCAAATCCCTAAAAGCCATCCCAGTTCGGATTGCAGGCTGAAACCCGCCTGTATGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTCGGGAACACCCGAAGTCCGTAGCCTAACCGCAAGGAGGGCGCGGCCGAAGGTGGGTTCGATAATTGGGGTGAAGTCGTAACAAGGTAG>SEQ ID NO: 94|NR_044054.1|Blautia wexlerae strain DSM 19850 16S ribosomalRNA gene, partial sequenceCAAGTCGAACGGGAATTANTTTATTGAAACTTCGGTCGATTTAATTTAATTCTAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTATACAGGGGGATAACAGTCAGAAATGGCTGCTAATACCGCATAAGCGCACAGAGCTGCATGGCTCAGTGTGAAAAACTCCGGTGGTATAAGATGGACCCGCGTTGGATTAGCTTGTTGGTGGGGTAACGGCCCACCAAGGCGACGATCCATAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGATAGTGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGGCATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATACTTGAGTGCCGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATAACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGTCGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCGCCTGACCGATCCTTAACCGGATCTTTCCTTCGGGACAGGCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTCAGTAGCCAGCATTTAAGGTGGGCACTCTGGGGAGACTGCCAGGGATAACCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGATTGTGAGATGGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCTAACTGCAAAGAAGGAGCTGCCGAAGGCGGGACCGATGACTGGGGTGAAGTCGTAACAAGGT>SEQ ID NO: 95|NR_027558.1|Anaerotruncus colihominis strain WAL 14565 16Sribosomal RNA gene, partial sequenceAACGGAGCTTACGTTTTGAAGTTTTCGGATGGATGAATGTAAGCTTAGTGGCGGACGGGTGAGTAACACGTGAGCAACCTGCCTTTCAGAGGGGGATAACAGCCGGAAACGGCTGCTAATACCGCATGATGTTGCGGGGGCACATGCCCCTGCAACCAAAGGAGCAATCCGCTGAAAGATGGGCTCGCGTCCGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGAAGACGGTCTTCGGATTGTAAACCTCTGTCTTTGGGGAAGAAAATGACGGTACCCAAAGAGGAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTAGAATGTTAAATCCATCGGCTCAACCGGTGGCTGCGTTCTAAACTGCCGTTCTTGAGTGAAGTAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTAGGTGTGGGGGGACTGACCCCTTCCGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGGCGTAATAGCCTAGAGAGTAGGTGAAGCCCTTCGGGGCATCCAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTATTAGTTGCTACGCAAGAGCACTCTAATGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCACTAAAACAGAGGGCGGCGACACCGCGAGGTGAAGCGAATCCCAGAAAAAGTGTCTCAGTTCAGATTGCAGGCTGCAACCCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTCCGGGTAACACCCGAAGCCAGTAG>SEQ ID NO: 96|NR_116747.1|Ruminococcus faecis strain Eg2 16S ribosomal RNAgene, partial sequenceATGCAAGTCGAACGAAGCACCTTGATTTGATTCTTCGGATGAAGATCTTGGTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACAGCACCGCATGGTGCAGGGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGCGATGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGATGTGAAAACCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTCAATCTAGAGTACCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGGCAGCAAAGCTGTTCGGTGCCGCAGCAAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGCTCTTGACATCTCCCTGACCGGCAAGTAATGTTGCCTTTCCTTCGGGACAGGGATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTTAGTAGCCAGCGGTTTGGCCGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAGCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCAGAACCGCGAGGTCGAGCAAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGAGCTGCCGAAG>SEQ ID NO: 97|NR_028883.1|Dorea longicatena strain 111-35 16S ribosomal RNAgene, partial sequenceTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACCGCTTCGTAATGGAAGTTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCAGGTTAAGCTGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAACTCGCGAGGGTAAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCATAACGCCCGAAGTCAGTGACCCAACCGTAAGG>SEQ ID NO: 98|NR_029164.1|Clostridium innocuum strain B-3 16S ribosomal RNAgene, partial sequenceATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTCTTCAGGAAGCTTGCTTCCAAAAAGACTTAGTGGCGAACGGGTGAGTAACACGTAGGTAACCTGCCCATGTGTCCGGGATAACTGCTGGAAACGGTAGCTAAAACCGGATAGGTATACGGAGCGCATGCTCTGTATATTAAAGCGCCCTTCAAGGCGTGAACATGGATGGACCTGCGACGCATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGCGTAGCCGGCCTGAGAGGGTAAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGAGTGAAGAAGGTCTTCGGATCGTAAAGCTCTGTTGTAAGTGAAGAACGGCTCATAGAGGAAATGCTATGGGAGTGACGGTAGCTTACCAGAAAGCCACGGCTAACTACGTGCCAGYAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCGTACTAAGTCTGTAGTAAAAGGCAATGGCTCAACCATTGTAAGCTATGGAAACTGGTATGCTGGAGTGCAGAAGAGGGCGATGGAATTCCATGTGTAGCGGTAAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGGTCGCCTGGTCTGTAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGAACTAAGTGTTGGAGAAATTCAGTGCTGCAGTTAACGCAATAAGTTCTCCGCCTGGGGAGTATGCACGCAAGTTNGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGNTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGGAAACAAATACCCTAGAGATAGGGGGATAATTATGGATCACACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCGCATGTTACCAGCATCAAGTTGGGGACTCATGCGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCTGGGCTACACACGTACTACAATGGCGACCACAAAGAGCAGCGACTTGGTGACAAGAAGCGAATCTCATAAAGATCGTCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCCGTCAAACCATGGGAGTCAGTAATACCCGAAGCCGGTGGCATAACCGTAAGGAGTGAGCCGTCGAAGGTAGGACCGA>SEQ ID NO: 99|NR104687.1|Blautia hansenii strain JCM 14655 16S ribosomal RNAgene, partial sequenceAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTATCATTGACTCTTCGGAAGATTTGATATTTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGAATAACAGTTAGAAATGGCTGCTAATGCCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTGAGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGGTGCAAAGCAGTTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCTGCCTGACCGTTCCTTAACCGGAGCTTTCCTTCGGGACAGGCAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTTAGTAGCCAGCAGTCCGGCTGGGCACTCTAGGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAAGCGGTGACGCTTAGCAAATCTCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCTTATGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAACC>SEQ ID NO: 100|NR_112933.1|Bacteroides cellulosilyticus strain JCM 15632 16Sribosomal RNAgene, partial sequenceAGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGACCTAGCAATAGGTTGATGGCGACCGGCGCACGGGTGAGTAACACGTATCCAACCTACCGGTTATTCCGGGATAGCCTTTCGAAAGAAAGATTAATACCGGATAGTATAACGAGAAGGCATCTTTTTGTTATTAAAGAATTTCGATAACCGATGGGGATGCGTTCCATTAGTTTGTTGGCGGGGTAACGGCCCACCAAGACATCGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGAAGGATGACTGCCCTATGGGTTGTAAACTTCTTTTATATGGGAATAAAGTGAGCCACGTGTGGCTTTTTGTATGTACCATACGAATAAGGATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACTATTAAGTCAGCTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGTCGTCTTGAGTGCAGTAGAGGTAGGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCGATTGCGAAGGCAGCTTACTGGACTGTAACTGACGCTGATGCTCGAAAGTGTGGGTATCAAACAGGATTAGATACCCTGGTAGTCCACACAGTAAACGATGAATACTCGCTGTTTGCGATATACAGCAAGCGGCCAAGCGAAAGCATTAAGTATTCCACCTGGGGAGTACGCCGGCAACGGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGAGGAACATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCCGGGCTTAAATTGCATCTGAATAATTTGGAAACAGATTAGCCGCAAGGCAGATGTGAAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTTAAGTGCCATAACGAGCGCAACCCTTATCTTTAGTTACTAACAGGTCATGCTGAGGACTCTAGAGAGACTGCCGTCGTAAGATGTGAGGAAGGTGGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCTACACACGTGTTACAATGGGGGGTACAGAAGGCAGCTACACAGCGATGTGATGCTAATCCCAAAAGCCTCTCTCAGTTCGGATTGGAGTCTGCAACCCGACTCCATGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCACGGCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAAGCCATGAAAGCCGGGGGTACCTGAAGTCCGTAACCGCAAGGAGCGGCCTAGGGTAAAACTGGTAATTGGGGCTAAGTCGTA>SEQ ID NO: 101|NR_112940.1|Bacteroides ovatus strain JCM 5824 16S ribosomalRNA gene, partial sequenceGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTTTAGTTTGCTTGCAAACTGAAGATGGCGACCGGCGCACGGGTGAGTAACACGTATCCAACCTGCCGATAACTCCGGAATAGCCTTTCGAAAGAAAGATTAATACCGGATAGCATACGAATATCGCATGATATTTTTATTAAAGAATTTCGGTTATCGATGGGGATGCGTTCCATTAGTTTGTTGGCGGGGTAACGGCCCACCAAGACTACGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGAAGGATGAAGGCTCTATGGGTCGTAAACTTCTTTTATATGGGAATAAAGTTTTCCACGTGTGGAATTTTGTATGTACCATATGAATAAGGATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCGATTGCGAAGGCAGCTCACTAGACTGTTACTGACACTGATGCTCGAAAGTGTGGGTATCAAACAGGATTAGATACCCTGGTAGTCCACACAGTAAACGATGAATACTCGCTGTTTGCGATATACAGTAAGCGGCCAAGCGAAAGCATTAAGTATTCCACCTGGGGAGTACGCCGGCAACGGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGAGGAACATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCCGGGCTTAAATTGCAACAGAATATATTGGAAACAGTATAGCCGTAAGGCTGTTGTGAAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTTAAGTGCCATAACGAGCGCAACCCTTATCTTTAGTTACTAACAGGTTATGCTGAGGACTCTAGAGAGACTGCCGTCGTAAGATGTGAGGAAGGTGGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCTACACACGTGTTACAATGGGGGGTACAGAAGGCAGCTACCTGGCGACAGGATGCTAATCCCAAAAACCTCTCTCAGTTCGGATCGAAGTCTGCAACCCGACTTCGTGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCATGGCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAAGCCATGAAAGCCGGGGGTACCTGAAGTACGTAACCGCAAGGAGCGTCCTAGGGTAAAACTGGTAATTGGGGCTA>SEQ ID NO: 102|NR_117563.1|Eubacterium fissicatena 16S ribosomal RNA gene,partial sequenceTAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTTACTTAGATTTCTTCGGATTGAAGAGTTTTGCGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACAGTACCGCATGGTACAGTGGGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTATGTAAGTCTGATGTGAAAACCCGGGGCTCAACCCCGGGACTGCATTGGAAACTATGTAACTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATCACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGCTCTTGACATCCCACTGACCGGCGTGTAATGGCGCCTTCCCTTCGGGGCAGTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTAGTAGCCAGCGGTTTGGCCGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAGCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCAATACCGCGAGGTTGAGCAAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGCGGGATCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTT>SEQ ID NO: 103|NR_104700.1|Blautia coccoides strain JCM 1395 16S ribosomalRNA gene, partial sequenceAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTAAGACAGATTTCTTCGGATTGAAGTCTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTGTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGTCCCGTAACGGGGGCTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCACATGATGGTGGGCACTCTAGGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGACAGCGATGTTGAGCGAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCTAACCGAAAGGAAGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAACC>SEQ ID NO: 104|NR_109014.1|Blautia faecis strain M25 16S ribosomal RNA gene,partial sequenceATAACAGCCAGAAATGACTGCTAATACCGCATAAGCGCACAGAACCGCATGGTTCGGTGTGAAAAACTCCGGTGGTATAAGATGGACCCGCGTTGGATTAGCTAGTTGGCAGGGCAGCGGCCTACCAAGGCGACGATCCATAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGATAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGATGTGAAAGGCAGGGGCTTAACCCCTGGACTGCATTGGAAACTGCTGTGCTTGAGTGCCGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCAGGGAGCACAGCTCTTTGGTGCCGCCGCAAACGCATTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAATCTTGACATCCCTCTGACCGGGACTTAACCGTCCCTTTCCTTCGGGACAGGGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTTAGTAGCCAGCACGCARTGGTGGGCACTCTGAGGAGACTGCCAGGGATAACCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAACCCGCGAGGGTGGGCAAATCTCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCG>SEQ ID NO: 105|NR_036928.1|Clostridium hathewayi strain 1313 16S ribosomalRNA gene, partial sequenceCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTTCAATGAAGTTTTCGGATGGATTTGAAATTGACTTAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTACACTGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGGCCGCATGGNCTGGTGTGAAAAACTCCGGNGGTGTAAGATGGACCCGCGTCTGATTAGGTAGTTGGNGGGGTAACGGCCCACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTAGCAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCTTTGGAAACTGTTAGACTTGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGGGGCAAAGCCCTTCGGTGCCGCCGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCACTGAAAACACNTTAACCGTGATCCCTCTTCGGAGCAGTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCGAGTAGAGTCGGGCACTCTGGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCAAAGGAGCGATCTGGAGCAAACCCCAAAAATAACGTCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAACGCCCGAAGTCAGTGACCCAACCGAAAGGAGGGAGCT>SEQ ID NO: 106|NR_113270.1|Blautia producta strain JCM 1471 16S ribosomalRNA gene, partial sequenceAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTAAGACGGATTTCTTCGGATTGAAGTCTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTGTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGTCCCGTAACGGGGACTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCACATGATGGTGGGCACTCTAGGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGACAGCGATGTTGAGCGAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCTAACCGAAAGGAAGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAACC>SEQ ID NO: 107|NR_104799.1|Anaerostipes hadrus strain DSM 3319 16S ribosomalRNA gene, partial sequenceTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTGCTTAACTGATCTTCTTCGGAATTGACGTTTTGTAGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCCTGTACAGGGGGATAACAGTCAGAAATGACTGCTAATACCGCATAAGACCACAGCACCGCATGGTGCAGGGGTAAAAACTCCGGTGGTACAGGATGGACCCGCGTCTGATTAGCTGGTTGGTGAGGTAACGGCTCACCAAGGCGACGATCAGTAGCCGGCTTGAGAGAGTGAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATCTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAAGTGAAAACCCAGGGCTTAACTCTGGGACTGCTTTTGAAACTGTCAGACTGGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACATCAGTGGCGAAGGCGGCTTACTGGACTGAAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGGCCGTAGAGGCTTCGGTGCCGCAGCCAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCTTCTGACCGGTCCTTAACCGGACCTTTCCTTCGGGACAGGAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTTAGTAGCCAGCATTTCAGGTGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAGAGGGAAGCAGCCTCGTGAGAGTGAGCAAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTC>SEQ ID NO: 108|NR_117142.1|Eubacterium fissicatena strain DSM 3598 16Sribosomal RNA gene, partial sequenceGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTTACTTAGATTTCTTCGGATTGAAGAGTTTTGCGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACAGTACCGCATGGTACAGTGGGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCAACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTATGTAAGTCTGATGTGAAAACCCGGGGCTCAACCCCGGGACTGCATTGGAAACTATGTAACTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATCACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGCTCTTGACATCCCACTGACCGGCGTGTAATGGCGCCTTCCCTTCGGGGCAGTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTAGTAGCCAGCGGTTTGGCCGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAGCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCAATACCGCGAGGTTGAGCAAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGCGGGATCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT>SEQ ID NO: 109|NR_117147.1|Eubacterium contortum strain DSM 3982 16Sribosomal RNA gene, partial sequenceTTTGATCCTGGCTCAGGATGAACGCTGGCGACGTGCTTAACACATGCAAGTCGAGCGAAGCACTTTACTTTGATTTCTTCGGAATGAAAGGTTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACAGTACCGCATGGTACAGTGGGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTATGTAAGTCTGATGTGAAAACCCGGGGCTCAACCCCGGGACTGCATTGGAAACTATGTAACTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGCTCTTGACATCCCCCTGACCGGCGTGTAATGGTGCCTTTCCTTCGGGACAGGGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTAGTAGCCAGCGGTTTGGCCGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAGCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCGAAGCCGTGAGGTGGAGCAAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAGGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCT>SEQ ID NO: 110|NR_113410.1|Clostridium bolteae strain JCM 12243 16Sribosomal RNA gene, partial sequenceTTTTAATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGATAACCTGCCTCACACTGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTACCGCATGGTACAGTGTGAAAAACTCCGGTGGTGTGAGATGGATCCGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGCAAGTCTGAAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGTTTTGCTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGGGGGCAAAGCCCTTCGGTGCCGTCGCAAACGCAGTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTCTTGACCGGCGTGTAACGGCGCCTTCCCTTCGGGGCAAGAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCAGGTAAAGCTGGGCACTCTAGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGACAGTGATGTGGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGCAACGCCCGAAGTCAGTGACCCAACTCGCAAGAGAGGGAGCTGCCGAAGGCGGGGCAGGTAACTGGGGTGAAGTC>SEQ ID NO: 111|NR_041960.1|Blautia luti strain BInIX 16S ribosomal RNA gene,complete sequenceGTGGGTAACCTGCCTTATACAGGGGGATAACAGTCAGAAATGACTGCTAATACCGCATAAGCGCACAGAGCTGCATGGCTCCGGTGTGAAAAACTCCGGTGGTATAAGATGGACCCGCGTTGGATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGACGATCCATAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGACAAGTCTGATGTGAAAGGCTGGGGCTCAACCCCGGGACTGCATTGGAAACTGCCCGTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCGGTAAACGATGAATCCTAGGTGTCGGGGAGCAAANNNNTTCGGTGCCGCCGCAAACGCATTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGAGTATGTATGGTACTTTTCCTTCGGGAGAGAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCCCAGTAGCCAGCGGTTCGGCCGGGCACTCTGAGGAGACTGCCAGGGATAACCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGCCTGCGAGGGTGGGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCTAACT>SEQ ID NO: 112|NR_074306.1|Acidaminococcus intestini RyC-MR95 strainRyC-MR95 16S ribosomal RNA, complete sequenceCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAACTTATTTCGGTAAGTTCTTAGTGGCGAACGGGTGAGTAACGCGTGGGCAACCTGCCCTCCAGTTGGGGACAACATTCCGAAAGGGATGCTAATACCGAATGTCCTCCCTCCTCCGCATGGAGGAGGGAGGAAAGATGGCCTCTGCTTGCAAGCTATCGCTGGAAGATGGGCCCGCGTCTGATTAGCTAGTTGGTGGGGTAACGGCTCACCAAGGCGATGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTCTTCGGATTGTAAAACTCTGTTGTTAGGGACGAAAGCACCGTGTTCGAACAGGTCATGGTGTTGACGGTACCTAACGAGGAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGGCTTTTAAGTCTGACGTGAAAATGCGGGGCTTAACCCCGTATGGCGTTGGATACTGGAAGTCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGTAGCGGTGAAATGCGTAGATATTGGGAGGAACACCAGTGGCGAAGGCGCCTTTCTGGACTGTGTCTGACGCTGAGATGCGAAAGCCAGGGTAGCAAACGGGATTAGATACCCCGGTAGTCCTGGCCGTAAACGATGGATACTAGGTGTAGGAGGTATCGACCCCTTCTGTGCCGGAGTTAACGCAATAAGTATCCCGCCTGGGGACTACGATCGCAAGATTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATTGAGTGAAAGACCTAGAGATAGGTCCCTCCCTTCGGGGACACGAAAACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTATGTTACCAGCGCGTAAAGGCGGGGACTCATAGGAGACTGCCAGGGATAACTTGGAGGAAGGCGGGGATGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTACACACGTACTACAATGGTCGGCAACAAAGGGCAGCGAAACCGCGAGGTGGAGCAAATCCCAGAAACCCGACCCCAGTTCGGATCGTAGGCTGCAACCCGCCTACGTGAAGTTGGAATCGCTAGTAATCGCAGGTCAGCATACTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAAAGTTGGTAACACCCGAAGCCGGTGAGATAACCTTTTAGGAGTCAGCTGTCTAAGGTGGGGCCGATGATTGGGGTGAAGTCGTAACAAGGTAGC>SEQ ID NO: 113|NR_074399.1|Ruminococcus albus strain 7 16S ribosomal RNAgene, complete sequenceAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGCGAAAGAGTGCTTGCACTCTCTAGCTAGTGGCGGACGGGTGAGTAACACGTGAGCAATCTGCCTTTCGGAGAGGGATACCAATTGGAAACGATTGTTAATACCTCATAACATAACGAAGCCGCATGACTTTGTTATCAAATGAATTTCGCCGAAAGATGAGCTCGCGTCTGATTAGGTAGTTGGTGAGGTAACGGCCCACCAAGCCGACGATCAGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGATGCCGCGTGAGGGAAGAAGGTTTTAGGATTGTAAACCTCTGTCTTTGGGGACGATAATGACGGTACCCAAGGAGGAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATTGCAAGTCAGGTGTGAAATTTAGGGGCTTAACCCCTGAACTGCACTTGAAACTGTAGTTCTTGAGTGAAGTAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACATCAGTGGCGAAGGCGGCTTACTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTAGGTGTGGGGGGACTGACCCCTTCCGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGTACGCATAGCATAGAGATATGTGAAATCCCTTCGGGGACGTATAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGGTTAAGTCCCGCAACGAGCGCAACCCTTACTGTTAGTTGCTACGCAAGAGCACTCTAGCAGGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCTGTTAACAGAGGGAAGCAAAACAGTGATGTGGAGCAAAACCCTAAAAGCAGTCTTAGTTCGGATTGTAGGCTGCAACCCGCCTACATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGCCATGGGAGTCGGTAACACCCGAAGCCTGTGTTCTAACCGCAAGGAGGAAGCAGTCGAAGGTGGGATTGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCT>SEQ ID NO: 114|NR_074634.1|Eubacterium rectale strain ATCC 33656 16Sribosomal RNA gene, complete sequenceAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTTATTTGATTTCCTTCGGGACTGATTATTTTGTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGTACAGGGGGATAACAGTTGGAAACGGCTGCTAATACCGCATAAGCGCACGGCATCGCATGATGCAGTGTGAAAAACTCCGGTGGTATAAGATGGACCCGCGTTGGATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGACGATCCATAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGCGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGATAATGACGGTACCTGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTTGGGAAGCATTGCTTCTCGGTGCCGTCGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTTCTGACCGGTACTTAACCGTACCTTCTCTTCGGAGCAGGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTAGTAGCCAGCGGTTCGGCCGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGACTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAAGCTGTGAAGCCGAGCAAATCTCAAAAATAACGTCTCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGGAATGCCCGAAGCCAGTGACCTAACCGAAAGGAAGGAGCTGTCGAAGGCAGGCTCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCT>SEQ ID NO: 115|NR_074928.1|Acidaminococcus fermentans strain DSM 20731 16Sribosomal RNA gene, complete sequenceAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAACTTTCTTCGGAATGTTCTTAGTGGCGAACGGGTGAGTAACGCGTAGGCAACCTGCCCTCTGGTTGGGGACAACATTCCGAAAGGGATGCTAATACCGAATGAGATCCTCTTTCCGCATGGAGAGAGGATGAAAGATGGCCTCTACTTGTAAGCTATCGCCAGAAGATGGGCCTGCGTCTGATTAGCTAGTAGGTGAGGTAACGGCTCACCTAGGCGATGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCCTTCGGGTTGTAAAACTCTGTTGTCAGGGACGAAAGCACCGATCTATAATACATTTTGGTGTTGACGGTACCTGACGAGGAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGGCTTTTAAGTCCGACGTGAAAATGCGGGGCTTAACCCCGTATGGCGTTGGATACTGGAAGTCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGTAGCGGTGAAATGCGTAGATATTGGGAGGAACACCAGTGGCGAAGGCGCCTTTCTGGACTGTGTCTGACGCTGAGATGCGAAAGCCAGGGTAGCAAACGGGATTAGATACCCCGGTAGTCCTGGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTGCCGGAGTTAACGCAATAAGTACCCCGCCTGGGGACTACGATCGCAAGATTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATTGAGTGAAAGACCCAGAGATGGGTCCCCTTCTTCGGAAGCACGAAAACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTATGTTACCAGCACGTAATGGTGGGGACTCATAGGAGACTGCCAGGGATAACCTGGAGGAAGGCGGGGATGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTACACACGTACTACAATGGTCGGCAACAAAGGGCAGCGAAGCCGCGAGGCGGAGCCAATCCCAGAAACCCGACCCCAGTTCGGATCGCAGGCTGCAACCCGCCTGCGTGAAGTTGGAATCGCTAGTAATCGCAGGTCAGCATACTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAAAGTTGGTAACACCCGAAGCCGGTGAGATAACCTTTTAGGAGTCAGCTGTCTAAGGTGGGGCCGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTTCGAGAACGAGCGGCTGGATCACCT>SEQ ID NO: 116|NR_114326.1|Fusicatenibacter saccharivorans strain HT03-1116S ribosomal RNAgene, partial sequenceTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTAAGAAGATTYTTCGGATGATTCTTGACTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTGACCTGCCCCATACCGGGGGATAACAGCTGGAAACGGCTGCTAATACCGCATAAGCGCACAGAGCTGCATGGCTCGGTGTGAAAAACTCCGGTGGTATGGGATGGGCCCGCGTCTGATTAGGCAGTTGGCGGGGTAACGGCCCACCAAACCGACGATCAGTAGCCGGCCTGAGAGGGCGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGCGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGATAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGGGAGCAAAGCTCTTCGGTGCCGCCGCAAACGCATTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCGATGACCGGCCCGTAACGGGGCCTTCTCTTCGGAGCATTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTCAGTAGCCAGCAGGTAAAGCTGGGCACTCTGTGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATCTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCAAAGCCGCGAGGTGGAGCAAATCCCAAAAATAACGTCTCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAACGCCCGAAGTCAGTGACCCAACCTTTTA>SEQ ID NO: 117|NR_102884.1|Ruminococcus champanellensis strain 18P13 16Sribosomal RNAgene, complete sequenceAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGAGATAAAGACTTCGGTTTTTATCTTAGTGGCGGACGGGTGAGTAACACGTGAGCAACCTGCCTCTGAGAGAGGGATAGCTTCTGGAAACGGATGGTAATACCTCATAACATAGCGGTACCGCATGATACTGCTATCAAAGATTTATCGCTCAGAGATGGGCTCGCGTCTGATTAGCTAGATGGTGAGGTAACGGCTCACCATGGCGACGATCAGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGATGCCGCGTGGAGGAAGAAGGTTTTCGGATTGTAAACTCCTGTCTTAAGGGACGATAATGACGGTACCTTAGGAGGAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATTGCAAGTCAGATGTGAAAACTATGGGCTTAACCCATAGACTGCATTTGAAACTGTAGTTCTTGAGTGAAGTAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACATCGGTGGCGAAGGCGGCTTACTGGGCTTTTACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGATTACTAGGTGTGGGGGGACTGACCCCTTCCGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAAAACCTTACCAGGTCTTGACATCGAGTGAATGATCTAGAGATAGATCAGTCCTTCGGGACACAAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTACCTTTAGTTGCTACGCAAGAGCACTCTAGAGGGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCAATGAACAGAGGGAAGCAATACAGTGATGTGGAGCAAATCCCCAAAAATTGTCCCAGTTCAGATTGTAGGCTGCAACTCGCCTACATGAAGTCGGAATTGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTCGGTAACACCCGAAGCCAGTAGCCTAACCGCAAGGAGGGCGCTGTCGAAGGTGGGATTGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCT>SEQ ID NO: 118|NR_102971.1|Bifidobacterium bifidum S17 strain S17 16Sribosomal RNA, complete sequenceTTTTTGTGGAGGGTTCGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCATCGGGCTTTGCTTGGTGGTGAGAGTGGCGAACGGGTGAGTAATGCGTGACCGACCTGCCCCATGCTCCGGAATAGCTCCTGGAAACGGGTGGTAATGCCGGATGTTCCACATGATCGCATGTGATTGTGGGAAAGATTCTATCGGCGTGGGATGGGGTCGCGTCCTATCAGCTTGTTGGTGAGGTAACGGCTCACCAAGGCTTCGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACATTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGAGGGATGGAGGCCTTCGGGTTGTAAACCTCTTTTGTTTGGGAGCAAGCCTTCGGGTGAGTGTACCTTTCGAATAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGCTCGTCGCGTCCGGTGTGAAAGTCCATCGCTTAACGGTGGATCTGCGCCGGGTACGGGCGGGCTGGAGTGCGGTAGGGGAGACTGGAATTCCCGGTGTAACGGTGGAATGTGTAGATATCGGGAAGAACACCGATGGCGAAGGCAGGTCTCTGGGCCGTCACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGACGCTGGATGTGGGGCACGTTCCACGTGTTCCGTGTCGGAGCTAACGCGTTAAGCGTCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGAAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGCGGATTAATTCGATGCAACGCGAAGAACCTTACCTGGGCTTGACATGTTCCCGACGACGCCAGAGATGGCGTTTCCCTTCGGGGCGGGTTCACAGGTGGTGCATGGTCGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCCCGTGTTGCCAGCACGTTATGGTGGGAACTCACGGGGGACCGCCGGGGTTAACTCGGAGGAAGGTGGGGATGACGTCAGATCATCATGCCCCTTACGTCCAGGGCTTCACGCATGCTACAATGGCCGGTACAGCGGGATGCGACATGGCGACATGGAGCGGATCCCTGAAAACCGGTCTCAGTTCGGATCGGAGCCTGCAACCCGGCTCCGTGAAGGCGGAGTCGCTAGTAATCGCGGATCAGCAACGCCGCGGTGAATGCGTTCCCGGGCCTTGTACACACCGCCCGTCAAGTCATGAAAGTGGGCAGCACCCGAAGCCGGTGGCCTAACCCCTTGTGGGATGGAGCCGTCTAAGGTGAGGCTCGTGATTGGGACTAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATCACCTCCTTTCT>SEQ ID NO: 119|NR_102980.1|Megasphaera elsdenii strain DSM 20460 16Sribosomal RNA gene, complete sequenceAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAAGAGATGAGAAGCTTGCTTCTTATCAATTCGAGTGGCAAACGGGTGAGTAACGCGTAAGCAACCTGCCCTTCAGATGGGGACAACAGCTGGAAACGGCTGCTAATACCGAATACGTTCTTTTTGTCGCATGGCAGAGGGAAGAAAGGGAGGCTCTTCGGAGCTTTCGCTGAAGGAGGGGCTTGCGTCTGATTAGCTAGTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAACGATGACGGCCTTCGGGTTGTAAAGTTCTGTTATACGGGACGAATGGCGTAGCGGTCAATACCCGTTACGAGTGACGGTACCGTAAGAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCAGGCGGCGTCGTAAGTCGGTCTTAAAAGTGCGGGGCTTAACCCCGTGAGGGGACCGAAACTGCGATGCTAGAGTATCGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAAGCGGCTTTCTGGACGACAACTGACGCTGAGGCGCGAAAGCCAGGGGAGCAAACGGGATTAGATACCCCGGTAGTCCTGGCCGTAAACGATGGATACTAGGTGTAGGAGGTATCGACCCCTTCTGTGCCGGAGTTAACGCAATAAGTATCCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGCCTTGACATTGATTGCTATGGATAGAGATATCCAGTTCCTCTTCGGAGGACAAGAAAACAGGTGGTGCACGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCTGTTACCAGCGGTTCGGCCGGGGACTCAGGAGAGACTGCCGCAGACAATGCGGAGGAAGGCGGGGATGACGTCAAGTCATCATGCCCCTTATGGCTTGGGCTACACACGTACTACAATGGCTCTTAATAGAGGGAAGCGAAGGAGCGATCCGGAGCAAACCCCAAAAACAGAGTCCCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCAGGAATCGCTAGTAATCGCAGGTCAGCATACTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAAAGTCATTCACACCCGAAGCCGGTGAGGTAACCTTTTGGAGCCAGCCGTCGAAGGTGGGGGCGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCT>SEQ ID NO: 120|NR_044645.2|Dorea formicigenerans strain ATCC 27755 16Sribosomal RNAgene, complete sequenceTTAAACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACATAAGTTTGATTCTTCGGATGAAGACTTTTGTGACTGAGCGGCGGACGNNNGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGYTAGAAATGGCTGCTAATACCGCATAAGACCACAGTACTGCATGGTACAGTGNNNAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGAGGTAACGGCCCACCNAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCNNGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGNGGTAATACGTAGGGGGNNAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAAGTGAAAGGCATGGGCTCAACCTGTGGACTGCTTTGGAAACTGTGCAGCTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCNTACTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTAGCAAAGCTATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGNCCNGCACAAGCGGTGGAGCATGTGGTTTAATTCGAANNAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACCGCTTCGTAATGGAAGYTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCAGCATTTAGGATGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTNNAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAGAGGGAGGCAGAGCCGCGAGGCCGAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGAAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGT>SEQ ID NO: 121|NR_118643.1|Eisenbergiella tayi strain B086562 16S ribosomalRNA gene, partial sequenceGGTATAACTTAGTGGCGGACGGGTGAGTAACGCGTGGGAAACCTGCCCTGTACCGGGGGATAACACTTAGAAATAGGTGCTAATACCGCATAAGCGCACGGAACCGCATGGTTCCGTGTGAAAAACTCCGGTGGTACAGGATGGTCCCGCGTCTGATTAGCCAGTTGGCAGGGTAACGGCCTACCAAAGCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGCCAGATGTGAAAACCCAGGGCTCAACCTTGGGATTGCATTTGGAACTGCCAGGCTGGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCGGTAAACGATGATTGCTAGGTGTAGGTGGGTATGGACCCATCGGTGCCGCAGCTAACGCAATAAGCAATCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCAATGACGCACCTGTAAAGAGGTGTTCCCTTCGGGGCATTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTCTTAGTAGCCAGCAGGTAAAGCTGGGCACTCTAAGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGACAGTGATGTGGAGCAAATCYCAGAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGAAATGCCCGAAGTCTGTGACCTAACCGAAAGGGAGGAGCAGCCGAAGGCAGGTCTGATAACTGGGGTGAAGTCGTAA>SEQ ID NO: 122|NR_118730.1|Clostridium symbiosum strain ATCC 14940 16Sribosomal RNAgene, partial sequenceAAACATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTTAACGGAAGTTTTCGGATGGAAGTTGAATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGTACTGGGGGACAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTATTGCATGATACAGTGTGAAAAACTCCGGTGGTACAAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCNNAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGNNAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAAGTGAAAGCCCGCGNCTCAACTGCGGNNCTGCTTTGGAAACTGTTTAACTGGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACNAGTGGCGAAGGCGACTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTTGGGGAGCAAAGCTCTTCGGTGCCGTCGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCNGCACAAGCGGTGGAGCATGTGGTTTAATTCGAANNAACGCGAAGAACCTTACCAGGTCTTGACATCGACTCGACGGGGGAGTAACGTCCCNNTNCCTTCGGGGCGGAGAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTNAGTCCCGCAACGAGCGCAACCCTTATTCTAAGTAGCCAGCGGTTCGGCCGGGAACTCTTGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCNAATCATCATGCCCCTTATGATCTGGGCTACACACGTGCTACAATGGCGTAAACANAGAGAAGCAAGACCGCGAGGTGGAGCAAATCTCAAAAATAACGTCTCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGNNCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGCGGGACCGANAACNNGGG>SEQ ID NO: 123|NR_113243.1|Erysipelatoclostridium ramosum strain JCM 129816S ribosomal RNA gene, partial sequenceAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCACTTGTGCTCGAGTGGCGAACGGGTGAGTAATACATAAGTAACCTGCCCTAGACAGGGGGATAACTATTGGAAACGATAGCTAAGACCGCATAGGTACGGACACTGCATGGTGACCGTATTAAAAGTGCCTCAAAGCACTGGTAGAGGATGGACTTATGGCGCATTAGCTGGTTGGCGGGGTAACGGCCCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGAAGGAAGAAGGTTTTCGGATTGTAAACTTCTGTTATAAAGGAAGAACGGCGGCTACAGGAAATGGTAGCCGAGTGACGGTACTTTATTAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCAGCAAGGGTCTGTGGTGAAAGCCTGAAGCTTAACTTCAGTAAGCCATAGAAACCAGGCAGCTAGAGTGCAGGAGAGGATCGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGACGATCTGGCCTGCAACTGACGCTCAGTCCCGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGTACTAAGTGTTGGATGTCAAAGTTCAGTGCTGCAGTTAACGCAATAAGTACTCCGCCTGAGTAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATACTCATAAAGGCTCCAGAGATGGAGAGATAGCTATATGAGATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACCATCATTAAGTTGGGGACTCTAGCGAGACTGCCAGTGACAAGCTGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTGCAGAGGGAAGCGAAGCCGCGAGGTGAAGCAAAACCCATAAAACCATTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTTGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTGATAACACCCGAAGCCGGTGGCCTAACCGCAAGGAAGGAGCTGTCTAAGGTGGGATTGATGATTGGGGTGAAGTCGTAACAAGGTAACC >SEQ ID NO: 124 IPROKKA_00507 16S ribosomal RNA gene |VE202-7ATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTAAAGGAAGTTTTCGGATGGAATTTGATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGATAACCTGCCTCACACTGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTACCGCATGGTACGGTGTGAAAAACTCCGGTGGTGTGAGATGGATCCGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGTGTAAAGGGAGCGTAGACGGCGAAGCAAGTCTGAAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGTTTTGCTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGGGGGCAAAGCCCTTCGGTGCCGTCGCAAACGCAGTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTCTTGACCGGCGTGTAACGGCGCCTTCCCTTCGGGGCAAGAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCAGGTAGAGCTGGGCACTCTAGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGACAGTGATGTGGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGCAACGCCCGAAGTCAGTGACCCAACTCGCAAGAGAGGGAGCTGCCGAAGGCGGGGCAGGTAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 125 IPROKKA_00709 16S ribosomal RNA gene |VE202-7ATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTAAAATGAAGTTTTCGGATGGATTTTTGATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGATAACCTGCCTCACACTGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTACCGCATGGTACGGTGTGAAAAACTCCGGTGGTGTGGGATGGATCCGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGCAAGTCTGAAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGTTTTGCTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGGGGGCAAAGCCCTTCGGTGCCGTCGCAAACGCAGTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTCTTGACCGGCGTGTAACGGCGCCTTCCCTTCGGGGCAAGAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCAGGTAAAGCTGGGCACTCTAGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGACAGTGATGTGGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGCAACGCCCGAAGTCAGTGACCCAACTCGCAAGAGAGGGAGCTGCCGAAGGCGGGGCAGGTAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 126 IPROKKA_01766 16S ribosomal RNA gene |VE202-7ATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTAAAATGAAGTTTTCGGATGGATTTTTGATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGATAACCTGCCTCACACTGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTACCGCATGGTACGGTGTGAAAAACTCCGGTGGTGTGAGATGGATCCGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGCAAGTCTGAAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGTTTTGCTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGGGGCAAAGCCCTTCGGTGCCGTCGCAAACGCAGTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTCTTGACCGGCGTGTAACGGCGCCTTCCCTTCGGGGCAAGAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCAGGTAAAGCTGGGCACTCTAGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGACAGTGATGTGGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGCAACGCCCGAAGTCAGTGACCCAACTCGCAAGAGAGGGAGCTGCCGAAGGCGGGGCAGGTAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 127 IPROKKA_01779 16S ribosomal RNA gene |VE202-7ATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTAAAATGAAGTTTTCGGATGGATTTTTGATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGATAACCTGCCTCACACTGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTACCGCATGGTACGGTGTGAAAAACTCCGGTGGTGTGAGATGGATCCGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGCAAGTCTGAAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGTTTTGCTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGGGGCAAAGCCCTTCGGTGCCGTCGCAAACGCAGTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTCTTGACCGGCGTGTAACGGCGCCTTCCCTTCGGGGCAGGAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCAGGTAGAGCTGGGCACTCTAGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGACAGTGATGTGGAGCAAATCCCAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGCAACGCCCGAAGTCAGTGACCCAACTCGCAAGAGAGGGAGCTGCCGAAGGCGGGGCAGGTAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 128 IPROKKA_05926 16S ribosomal RNA gene |VE202-7ATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTAAAATGAAGTTTTCGGATGGATTTTAATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGATAACCTGCCTCACACTGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTACCGCATGGTACGGTGTGAAAAACTCCGGTGGTGTGAGATGGATCCGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGCAAGTCTGAAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGTTTTGCTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGGGGGCAAAGCCCTTCGGTGCCGTCGCAAACGCAGTAAGCATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTCTTGACCGGCGTGTAACGGCGCCTTCCCTTCGGGGCAAGAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTAGTAGCCAGCAGGTAAAGCTGGGCACTCTAGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGACAGTGATGTGGAGCAAATCCCAAAATAACGTCCCAGTTCGGACTGTAGTCTGCAACCCGACTACACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGCAACGCCCGAAGTCAGTGACCCAACTCGCAAGAGAGGGAGCTGCCGAAGGCGGGGCAGGTAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 129 |PROKKA_01784 16S ribosomal RNA geneTCAAAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTACGTTTTGAAGTTTTCGGATGGATGAATGTAAGCTTAGTGGCGGACGGGTGAGTAACACGTGAGCAACCTGCCTTTCAGAGGGGGATAACAGCCGGAAACGGCTGCTAATACCGCATGATGTTGCGGGGGCACATGCCCCTGCAACCAAAGGAGCAATCCGCTGAAAGATGGGCTCGCGTCCGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGAAGACGGTCTTCGGATTGTAAACCTCTGTCTTTGGGGAAGAAAATGACGGTACCCAAAGAGGAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTAGAATGTTAAATCCATCGGCTCAACCGGTGGCTGCGTTCTAAACTGCCGTTCTTGAGTGAAGTAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTAGGTGTGGGGGGACTGACCCCTTCCGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGGATGCATAGCCTAGAGATAGGTGAAGCCCTTCGGGGCATCCAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTATTAGTTGCTACGCAAGAGCACTCTAATGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCACTAAAACAGAGGGCGGCGACACCGCGAGGTGAAGCGAATCCCGAAAAAGTGTCTCAGTTCAGATTGCAGGCTGCAACCCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTCGGTAACACCCGAAGCCAGTAGCCTAACCGCAAGGGGGGCGCTGTCGAAGGTGGGATTGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 130 |PROKKA_01864 16S ribosomal RNA geneTCAAAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTACGTTTTGAAGTTTTCGGATGGACGAATGTAAGCTTAGTGGCGGACGGGTGAGTAACACGTGAGCAACCTGCCTTTCAGAGGGGATAACAGCCGGAAACGGCTGCTAATACCGCATGATGTTGCGGGGGCACATGCCCCTGCAACCAAAGGAGCAATCCGCTGAAAGATGGGCTCGCGTCCGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGAAGACGGTCTTCGGATTGTAAACCTCTGTCTTTGGGGAAGAAAATGACGGTACCCAAAGAGGAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTAGAATGTTAAATCCATCGGCTCAACCGGTGGCTGCGTTCTAAACTGCCGTTCTTGAGTGAAGTAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTAGGTGTGGGGGGACTGACCCCTTCCGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGGATGCATAGCCTAGAGATAGGTGAAGCCCTTCGGGGCATCCAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTATTAGTTGCTACGCAAGAGCACTCTAATGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCACTAAAACAGAGGGCGGCGACACCGCGAGGTGAAGCGAATCCCGAAAAAGTGTCTCAGTTCAGATTGCAGGCTGCAACCCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTCGGTAACACCCGAAGCCAGTAGCCTAACCGCAAGGGGGGCGCTGTCGAAGGTGGGATTGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 131 |PROKKA_02671 16S ribosomal RNA geneTCAAAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTACGTTTTGAAGTTTTCGGATGGATGAATGTAAGCTTAGTGGCGGACGGGTGAGTAACACGTGAGCAACCTGCCTTTCAGAGGGGGATAACAGCCGGAAACGGCTGCTAATACCGCATGATGTTGCGGGGGCACATGCCCCTGCAACCAAAGGAGCAATCCGCTGAAAGATGGGCTCGCGTCCGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGAAGACGGTCTTCGGATTGTAAACCTCTGTCTTTGGGGAAGAAAATGACGGTACCCAAAGAGGAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTAGAATGTTAAATCCATCGGCTCAACCGGTGGCTGCGTTCTAAACTGCCGTTCTTGAGTGAAGTAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTAGGTGTGGGGGGACTGACCCCTTCCGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGGATGCATAGCCTAGAGATAGGTGAAGCCCTTCGGGGCATCCAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTATTAGTTGCTACGCAAGAGCACTCTAATGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCACTAAAACAGAGGGCGGCGACACCGCGAGGTGAAGCGAATCCCGAAAAAGTGTCTCAGTTCAGATTGCAGGCTGCAACCCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTCGGTAACACCCGAAGCCAGTAGCCTAACCGCAAGGGGGGCGCTGTCGAAGGTGGGATTGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 132 |PROKKA_00690 16S ribosomal RNA geneTACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCTGTTTTCAGAATCTTCGGAGGAAGAGGACAGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGACCGCATGGTGTAGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAAAGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGAGTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATAGGCAAGTCTGGAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGCAGATCTGGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGTGTGCAAAGCACATCGGTGCCGCAGCAAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCGGATGACGGGCGAGTAATGTCGCCGTCCCTTCGGGGCGTCCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCAGCATATAAGGTGGGCACTCTGGAGAGACTGCCAGGGAGAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGAGGGTGACCTGGAGCGAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGCCAGTGACCCAACCTTAGAGGAGGGAGCTGTCGAAGGCGGGACGGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 133 |PROKKA_00991 16S ribosomal RNA geneTACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCTGTTTTCAGAATCTTCGGAGGAAGAGGACAGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGACCGCATGGTGTAGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAAAGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAGATGACGGTACCTGAGTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATAGGCAAGTCTGGAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGCAGATCTGGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGTGTGCAAAGCACATCGGTGCCGCAGCAAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCGGATGACGGGCGAGTAATGTCGCCGTCCCTTCGGGGCATCCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCAGCATATAAGGTGGGCACTCTGGAGAGACTGCCAGGGAGAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGAGGGTGACCTGAAGCGAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGCCAGTGACCCAACCTTAGAGGAGGGAGCTGTCGAAGGCGGGACGGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 134 |PROKKA_01948 16S ribosomal RNA geneTACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCTGTTTTCAGAATCTTCGGAGGAAGAGGACAGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGACCGCATGGTGTAGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAAAGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAGATGACGGTACCTGAGTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATAGGCAAGTCTGGAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGCAGATCTGGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGTGTGCAAAGCACATCGGTGCCGCAGCAAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCGGATGACGGGCGAGTAATGTCGCCGTCCCTTCGGGGCATCCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCAGCATATAAGGTGGGCACTCTGGAGAGACTGCCAGGGAGAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGAGGGTGACCTGGAGCGAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGCCAGTGACCCAACCTTAGAGGAGGGAGCTGTCGAAGGCGGGACGGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 135 |PROKKA_02310 16S ribosomal RNA geneTACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCTGTTTTCAGAATCTTCGGAGGAAGAGGACAGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGACCGCATGGTGTAGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAAAGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAGATGACGGTACCTGAGTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATAGGCAAGTCTGGAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGCAGATCTGGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGTGTGCAAAGCACATCGGTGCCGCAGCAAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCGGATGACGGGCGAGTAATGTCGCCGTCCCTTCGGGGCATCCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCAGCATATAAGGTGGGCACTCTGGAGAGACTGCCAGGGAGAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGAGGGTGACCTGAAGCGAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGCCAGTGACCCAACCTTAGAGGAGGGAGCTGTCGAAGGCGGGACGGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 136 |PROKKA_02993 16S ribosomal RNA geneTACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCTGTTTTCAGAATCTTCGGAGGAAGAGGACAGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGACCGCATGGTGTAGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAAAGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAGATGACGGTACCTGAGTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATAGGCAAGTCTGGAGTGAAAACCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGCAGATCTGGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGTGTGCAAAGCACATCGGTGCCGCAGCAAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCGGATGACGGGCGAGTAATGTCGCCGTCCCTTCGGGGCATCCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCAGCATATAAGGTGGGCACTCTGGAGAGACTGCCAGGGAGAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGAGGGTGACCTGGAGCGAATCCCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGCCAGTGACCCAACCTTAGAGGAGGGAGCTGTCGAAGGCGGGACGGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 137 |PROKKA_00436 16S ribosomal RNA geneATGAGAGTTTGATCCTAGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTTAACGGAAGTTTTCGGATGGAAGTTGAATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGTACTGGGGGACAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTATCGCATGATACAGTGTGAAAAACTCCGGTGGTACAAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAAGTGAAAGCCCGCGGCTCAACTGCGGGACTGCTTTGGAAACTGTTTAACTGGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGACTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTTGGGGAGCAAAGCTCTTCGGTGCCGTCGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGATCCGACGGGGGAGTAACGTCCCCTTCCCTTCGGGGCGGAGAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTCTAAGTAGCCAGCGGTTCGGCCGGGAACTCTTGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATCTGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAATCTCAAAAATAACGTCTCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 138 |PROKKA_00685 16S ribosomal RNA geneATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTTAACGGAAATTTTCGGATGGAAGTTGAATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGTACTGGGGGACAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTATCGCATGATACAGTGTGAAAAACTCCGGTGGTACAAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAAGTGAAAGCCCGCGGCTCAACTGCGGGACTGCTTTGGAAACTGTTTAACTGGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGACTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTTGGGGAGCAAAGCTCTTCGGTGCCGTCGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGATCCGACGGGGGAGTAACGTCCCCTTCCCTTCGGGGCGGAGAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTCTAAGTAGCCAGCGGTTCGGCCGGGAACTCTTGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATCTGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAATCTCAAAAATAACGTCTCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 139 |PROKKA_01171 16S ribosomal RNA geneATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTTAACGGAAGTTTTCGGATGGAAGTTGAATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGTACTGGGGGACAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTATCGCATGATACAGTGTGAAAAACTCCGGTGGTACAAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAAGTGAAAGCCCGCGGCTCAACTGCGGGACTGCTTTGGAAACTGTTTAACTGGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGACTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTTGGGGAGCAAAGCTCTTCGGTGCCGTCGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGATCCGACGGGGGAGTAACGTCCCCTTCCCTTCGGGGCGGAGAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTCTAAGTAGCCAGCGGTTCGGCCGGGAACTCTTGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATCTGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAATCTCAAAAATAACGTCTCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 140 |PROKKA_05969 16S ribosomal RNA geneATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTTAACGGAAGTTTTCGGATGGAAGTTGGATTGACTGAGTGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTTGTACTGGGGGACAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGTATCGCATGATACAGTGTGAAAAACTCCGGTGGTACAAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGTGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAAGTGAAAGCCCGCGGCTCAACTGCGGGACTGCTTTGGAAACTGTTTAACTGGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGACTTACTGGACGATAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTTGGGGAGCAAAGCTCTTCGGTGCCGTCGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGATCCGACGGGGGAGTAACGTCCCCTTCCCTTCGGGGCGGAGAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTCTAAGTAGCCAGCGGTTCGGCCGGGAACTCTTGGGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATCTGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAATCTCAAAAATAACGTCTCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 141 |PROKKA_00279 16S ribosomal RNA geneATCAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTAAGTGGATCTCTTCGGATTGAAACTTATTTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGGCTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGGCCCGTAACGGGGCCTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTTAGTAGCCAGCAGGTGAAGCTGGGCACTCTAGGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGACAGCGATGTTGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCTTTTAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 142 |PROKKA_01221 16S ribosomal RNA geneATCAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTAAGTGGATCTCTTCGGATTGAAACTTATTTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGGCTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTAGAGTGCCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGGCCCGTAACGGGGCCTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTTAGTAGCCAGCAGGTGAAGCTGGGCACTCTAGGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGGGACAGCGATGTTGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCTTACAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 143 |PROKKA_02318 16S ribosomal RNA geneATCAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTAAGTGGATCTCTTCGGATTGAAGCTTATTTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGGCTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGGCCCGTAACGGGGCCTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTTAGTAGCCAGCAGGTGAAGCTGGGCACTCTAGGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGACAGCGATGTTGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCTTACAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 144 |PROKKA_02336 16S ribosomal RNA geneATCAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTAAGCGGATCTCTTCGGATTGAAACTTATTTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGGCTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGGCCCGTAACGGGGCCTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTTAGTAGCCAGCAGGTGAAGCTGGGCACTCTAGGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGACAGCGATGTTGAGCAAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCTTACAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 145 |PROKKA_04947 16S ribosomal RNA geneATCAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTAAGTGGATCTCTTCGGATTGAAACTTATTTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGGCTGCTAATACCGCATAAGCGCACAGGACCGCATGGTCTGGTGTGAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGCCTGAGAGGGTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGAAGAAGTATCTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTAGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACATCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCAAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGGCCCGTAACGGGGCCTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTTAGTAGCCAGCAGGTGAAGCTGGGCACTCTAGGGAGACTGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGACAGCGATGTTGAGCAAATCCCAAAAATAACGTCCTAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCTTACAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 146 |PROKKA_00208 16S ribosomal RNA geneAACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTAAGTTTGATTCTTCGGATGAAGACTTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACCGCTTCGTAATGGAAGTTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCAGGTTAAGCTGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAACTCGCGAGGGTAAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 147 |PROKKA_00340 16S ribosomal RNA geneAACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTTGGAAAGATTCTTCGGATGATTTCCTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACCGCTTCGTAATGGAAGCTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCAGGTTAAGCTGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAACTCGCGAGGGTAAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 148 |PROKKA_01031 16S ribosomal RNA geneAACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTAAGTTTGATTCTTCGGATGAAGACTTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACCGCTTCGTAATGGAAGCTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCAGGTTAAGCTGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAACTCGCGAGGGTAAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 149 |PROKKA_01840 16S ribosomal RNA geneAACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTTGGAAAGATTCTTCGGATGATTTCCTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACCGCTTCGTAATGGAAGCTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCAGGTTAAGCTGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAACTCGCGAGGGTAAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 150 |PROKKA_02944 16S ribosomal RNA geneAACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTAAGTTTGATTCTTCGGATGAAGACTTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAGCCCGGGCTCAACCCCGGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACCGCTTCGTAATGGAAGTTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCAGGTTAAGCTGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAGGCAAACTCGCGAGGGTAAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 151 |PROKKA_04036 16S ribosomal RNA geneAACGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTTGGGAAGATTCTTCGGATGATTTCCTTTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACGGTACCGCATGGTACAGTGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTTTGATTAGGTAGTTGGTGGGGTAACGGCCTACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGCTGAGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGATCTTGACATCCCGATGACTGCTTCGTAATGGAAGTTTTTCTTCGGAACATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCTTCAGTAGCCAGCAGGTTAAGCTGGGCACTCTGGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAACTCGCGAGGGTAAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCAGATCAGAATGCTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGTGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 152 |PROKKA_00437 16S ribosomal RNA geneATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTTTCGAGGAAGCTTGCTTCCAAAGAGACTTAGTGGCGAACGGGTGAGTAACACGTAGGTAACCTGCCCATGTGTCCGGGATAACTGCTGGAAACGGTAGCTAAAACCGGATAGGTATACAGAGCGCATGCTCAGTATATTAAAGCGCCCATCAAGGCGTGAACATGGATGGACCTGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGATGATGCGTAGCCGGCCTGAGAGGGTAAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGAGTGAAGAAGGTCTTCGGATCGTAAAGCTCTGTTGTAAGTGAAGAACGGCTCATAGAGGAAATGCTATGGGAGTGACGGTAGCTTACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCGTACTAAGTCTGTAGTAAAAGGCAATGGCTCAACCATTGTAAGCTATGGAAACTGGTATGCTGGAGTGCAGAAGAGGGCGATGGAATTCCATGTGTAGCGGTAAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGGTCGCCTGGTCTGTAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGAACTAAGTGTTGGAGGAATTCAGTGCTGCAGTTAACGCAATAAGTTCTCCGCCTGGGGAGTATGCACGCAAGTGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGGAAACAAATACCCTAGAGATAGGGGGATAATTATGGATCACACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCGCATGTTACCAGCATCAAGTTGGGGACTCATGCGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCTGGGCTACACACGTACTACAATGGCGGCCACAAAGAGCAGCGACACAGTGATGTGAAGCGAATCTCATAAAGGTCGTCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCCGTCAAACCATGGGAGTCAGTAATACCCGAAGCCGGTGGCATAACCGTAAGGAGTGAGCCGTCGAAGGTAGGACCGATGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGGGAACGTGGGGATGGATCACCTCCTTT >SEQ ID NO: 153 |PROKKA_00896 16S ribosomal RNA geneATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTTTCGAGGAAGCTTGCTTCCAAAGAGACTTAGTGGCGAACGGGTGAGTAACACGTAGGTAACCTGCCCATGTGTCCGGGATAACTGCTGGAAACGGTAGCTAAAACCGGATAGGTATACAGAGCGCATGCTCAGTATATTAAAGCGCCCATCAAGGCGTGAACATGGATGGACCTGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGCGTAGCCGGCCTGAGAGGGTAAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGAGTGAAGAAGGTCTTCGGATCGTAAAGCTCTGTTGTAAGTGAAGAACGGCTCATAGAGGAAATGCTATGGGAGTGACGGTAGCTTACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCGTACTAAGTCTGTAGTAAAAGGCAATGGCTCAACCATTGTAAGCTATGGAAACTGGTATGCTGGAGTGCAGAAGAGGGCGATGGAATTCCATGTGTAGCGGTAAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGGTCGCCTGGTCTGTAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGAACTAAGTGTTGGAGGAATTCAGTGCTGCAGTTAACGCAATAAGTTCTCCGCCTGGGGAGTATGCACGCAAGTGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGGAAACAAATACCCTAGAGATAGGGGGATAATTATGGATCACACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCGCATGTTACCAGCATCAAGTTGGGGACTCATGCGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCTGGGCTACACACGTACTACAATGGCGGCCACAAAGAGCAGCGACACAGTGATGTGAAGCGAATCTCATAAAGGTCGTCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCCGTCAAACCATGGGAGTCAGTAATACCCGAAGCCGGTGGCATAACCGTAAGGAGTGAGCCGTCGAAGGTAGGACCGATGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGGGAACGTGGGGATGGATCACCTCCTTT >SEQ ID NO: 154 |PROKKA_02845 16S ribosomal RNA geneATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTTTCGAGGAAGCTTGCTTCCAAAGAGACTTAGTGGCGAACGGGTGAGTAACACGTAGGTAACCTGCCCATGTGTCCGGGATAACTGCTGGAAACGGTAGCTAAAACCGGATAGGTATACAGAGCGCATGCTCAGTATATTAAAGCGCCCATCAAGGCGTGAACATGGATGGACCTGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGATGATGCGTAGCCGGCCTGAGAGGGTAAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGAGTGAAGAAGGTCTTCGGATCGTAAAGCTCTGTTGTAAGTGAAGAACGGCTCATAGAGGAAATGCTATGGGAGTGACGGTAGCTTACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCGTACTAAGTCTGTAGTAAAAGGCAATGGCTCAACCATTGTAAGCTATGGAAACTGGTATGCTGGAGTGCAGAAGAGGGCGATGGAATTCCATGTGTAGCGGTAAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGGTCGCCTGGTCTGTAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGAACTAAGTGTTGGAGGAATTCAGTGCTGCAGTTAACGCAATAAGTTCTCCGCCTGGGGAGTATGCACGCAAGTGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGGAAACAAATACCCTAGAGATAGGGGGATAATTATGGATCACACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCGCATGTTACCAGCATCAAGTTGGGGACTCATGCGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCTGGGCTACACACGTACTACAATGGCGGCCACAAAGAGCAGCGACACAGTGATGTGAAGCGAATCTCATAAAGGTCGTCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCCGTCAAACCATGGGAGTCAGTAATACCCGAAGCCGGTGGCATAACCGTAAGGAGTGAGCCGTCGAAGGTAGGACCGATGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGGGAACGTGGGGATGGATCACCTCCTTT >SEQ ID NO: 155 |PROKKA_04164 16S ribosomal RNA geneATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTTTCGAGGAAGCTTGCTTCCAAAGAGACTTAGTGGCGAACGGGTGAGTAACACGTAGGTAACCTGCCCATGTGTCCGGGATAACTGCTGGAAACGGTAGCTAAAACCGGATAGGTATACAGAGCGCATGCTCAGTATATTAAAGCGCCCATCAAGGCGTGAACATGGATGGACCTGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGCGTAGCCGGCCTGAGAGGGTAAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGAGTGAAGAAGGTCTTCGGATCGTAAAGCTCTGTTGTAAGTGAAGAACGGCTCATAGAGGAAATGCTATGGGAGTGACGGTAGCTTACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCGTACTAAGTCTGTAGTAAAAGGCAATGGCTCAACCATTGTAAGCTATGGAAACTGGTATGCTGGAGTGCAGAAGAGGGCGATGGAATTCCATGTGTAGCGGTAAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGGTCGCCTGGTCTGTAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGAACTAAGTGTTGGAGGAATTCAGTGCTGCAGTTAACGCAATAAGTTCTCCGCCTGGGGAGTATGCACGCAAGTGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGGAAACAAATACCCTAGAGATAGGGGGATAATTATGGATCACACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCGCATGTTACCAGCATCAAGTTGGGGACTCATGCGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCTGGGCTACACACGTACTACAATGGCGACCACAAAGAGCAGCGACACAGTGATGTGAAGCGAATCTCATAAAGGTCGTCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCCGTCAAACCATGGGAGTCAGTAATACCCGAAGCCGGTGGCATAACCGCAAGGAGTGAGCCGTCGAAGGTAGGACCGATGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGGGAACGTGGGGATGGATCACCTCCTTT >SEQ ID NO: 156 |PROKKA_04921 16S ribosomal RNA geneATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTTTCGAGGAAGCTTGCTTCCAAAGAGACTTAGTGGCGAACGGGTGAGTAACACGTAGGTAACCTGCCCATGTGTCCGGGATAACTGCTGGAAACGGTAGCTAAAACCGGATAGGTATACAGAGCGCATGCTCAGTATATTAAAGCGCCCATCAAGGCGTGAACATGGATGGACCTGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGCGTAGCCGGCCTGAGAGGGTAAACGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGAGTGAAGAAGGTCTTCGGATCGTAAAGCTCTGTTGTAAGTGAAGAACGGCTCATAGAGGAAATGCTATGGGAGTGACGGTAGCTTACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCGTACTAAGTCTGTAGTAAAAGGCAATGGCTCAACCATTGTAAGCTATGGAAACTGGTATGCTGGAGTGCAGAAGAGGGCGATGGAATTCCATGTGTAGCGGTAAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGGTCGCCTGGTCTGTAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGAACTAAGTGTTGGAGGAATTCAGTGCTGCAGTTAACGCAATAAGTTCTCCGCCTGGGGAGTATGCACGCAAGTGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGGAAACAAATACCCTAGAGATAGGGGGATAATTATGGATCACACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCGCATGTTACCAGCATCAAGTTGGGGACTCATGCGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGCCTGGGCTACACACGTACTACAATGGCGGCCACAAAGAGCAGCGACACAGTGATGTGAAGCGAATCTCATAAAGGTCGTCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCCGTCAAACCATGGGAGTCAGTAATACCCGAAGCCGGTGGCATAACCGTAAGGAGTGAGCCGTCGAAGGTAGGACCGATGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGGGAACGTGGGGATGGATCACCTCCTTT >SEQ ID NO: 157 |PROKKA_00199 16S ribosomal RNA geneTATTGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCATGACGGAGGATTCGTCCAACGGATTGAGTTACCTAGTGGCGGACGGGTGAGTAACGCGTGAGGAACCTGCCTTGGAGAGGGGAATAACACTCCGAAAGGAGTGCTAATACCGCATGATGCAGTTGGGTCGCATGGCTCTGACTGCCAAAGATTTATCGCTCTGAGATGGCCTCGCGTCTGATTAGCTAGTAGGCGGGGTAACGGCCCACCTAGGCGACGATCAGTAGCCGGACTGAGAGGTTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGAAGGAAGAAGGCTTTCGGGTTGTAAACTTCTTTTGTCGGGGACGAAACAAATGACGGTACCCGACGAATAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTGGAGAGGCAATCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATACTAGGTGTGGGGGTCTGACCCCCTCCGTGCCGCAGTTAACACAATAAGTATCCCACCTGGGGAGTACGATCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGGCTTGACATCCCACTAACGAAGCAGAGATGCATTAGGTGCCCTTCGGGGAAAGTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTGTTAGTTGCTACGCAAGAGCACTCTAGCGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGTCCTGGGCCACACACGTACTACAATGGTGGTTAACAGAGGGAGGCAATACCGCGAGGTGGAGCAAATCCCTAAAAGCCATCCCAGTTCGGATTGCAGGCTGAAACCCGCCTGTATGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTCGGGAACACCCGAAGTCCGTAGCCTAACCGCAAGGAGGGCGCGGCCGAAGGTGGGTTCGATAATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 158 |PROKKA_00208 16S ribosomal RNA geneTATTGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCATGACGGAGGATTCGTCCAACGGATTGAGTTACCTAGTGGCGGACGGGTGAGTAACGCGTGAGGAACCTGCCTTGGAGAGGGGAATAACACTCCGAAAGGAGTGCTAATACCGCATGATGCAGTTGGGTCGCATGGCTCTGACTGCCAAAGATTTATCGCTCTGAGATGGCCTCGCGTCTGATTAGCTAGTAGGCGGGGTAACGGCCCACCTAGGCGACGATCAGTAGCCGGACTGAGAGGTTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGAAGGAAGAAGGCTTTCGGGTTGTAAACTTCTTTTGTCGGGGACGAAACAAATGACGGTACCCGACGAATAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTGGAGAGGCAATCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATACTAGGTGTGGGGGGTCTGACCCCCTCCGTGCCGCAGTTAACACAATAAGTATCCCACCTGGGGAGTACGATCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGGCTTGACATCCCACTAACGAAGCAGAGATGCATTAGGTGCCCTTCGGGGAAAGTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTGTTAGTTGCTACGCAAGAGCACTCTAGCGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGTCCTGGGCCACACACGTACTACAATGGTGGTTAACAGAGGGAGGCAATACCGCGAGGTGGAGCAAATCCCTAAAAGCCATCCCAGTTCGGATTGCAGGCTGAAACCCGCCTGTATGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTCGGGAACACCCGAAGTCCGTAGCCTAACCGCAAGGAGGGCGCGGCCGAAGGTGGGTTCGATAATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT >SEQ ID NO: 159 |PROKKA_04460 16S ribosomal RNA geneTATTGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCATGACGGAGGATTCGTCCAACGGATTGAGTTACCTAGTGGCGGACGGGTGAGTAACGCGTGAGGAACCTGCCTTGGAGAGGGGAATAACACTCCGAAAGGAGTGCTAATACCGCATAATGCAGTTGGGTCGCATGGCTCTGACTGCCAAAGATTTATCGCTCTGAGATGGCCTCGCGTCTGATTAGCTAGTAGGCGGGGTAACGGCCCACCTAGGCGACGATCAGTAGCCGGACTGAGAGGTTGACCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGAAGGAAGAAGGCTTTCGGGTTGTAAACTTCTTTTGTCAGGGACGAAACAAATGACGGTACCTGACGAATAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTGGAGAGGCAATCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATACTAGGTGTGGGGGGTCTGACCCCTCCGTGCCGCAGTTAACACAATAAGTATCCCACCTGGGGAGTACGATCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGGCTTGACATCCCACTAACGAAGCAGAGATGCATTAGGTGCCCTTCGGGGAAAGTGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTGTTAGTTGCTACGCAAGAGCACTCTAGCGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGTCCTGGGCCACACACGTACTACAATGGTGGTTAACAGAGGGAGGCAATACCGCGAGGTGGAGCAAATCCCTAAAAGCCATCCCAGTTCGGATTGCAGGCTGAAACCCGCCTGTATGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTCGGGAACACCCGAAGTCCGTAGCCTAACCGCAAGGAGGGCGCGGCCGAAGGTGGGTTCGATAATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT

This invention is not limited in its application to the details ofconstruction and the arrangement of components set forth in thefollowing description or illustrated in the drawings. The invention iscapable of other embodiments and of being practiced or of being carriedout in various ways. Also, the phraseology and terminology used hereinis for the purpose of description and should not be regarded aslimiting. The use of “including,” “comprising,” or “having,”“containing,” “involving,” and variations thereof herein, is meant toencompass the items listed thereafter and equivalents thereof as well asadditional items.

Unless otherwise defined herein, scientific and technical terms used inconnection with the present disclosure shall have the meanings that arecommonly understood by those of ordinary skill in the art. Further,unless otherwise required by context, singular terms shall includepluralities and plural terms hall include the singular. The methods andtechniques of the present disclosure are generally performed accordingto conventional methods well-known in the art. Generally, nomenclaturesused in connection with, and techniques of biochemistry, enzymology,molecular and cellular biology, microbiology, virology, cell or tissueculture, genetics and protein and nucleic chemistry described herein arethose well-known and commonly used in the art. The methods andtechniques of the present disclosure are generally performed accordingto conventional methods well known in the art and as described invarious general and more specific references that are cited anddiscussed throughout the present specification unless otherwiseindicated.

The present invention is further illustrated by the following Examples,which in no way should be construed as further limiting. The entirecontents of all of the references (including literature references,issued patents, published patent applications, and co-pending patentapplications) cited throughout this application are hereby expresslyincorporated by reference, in particular for the teaching that isreferenced hereinabove. However, the citation of any reference is notintended to be an admission that the reference is prior art.

EXAMPLES Example 1: Mouse Model of C. difficile Infection MouseHusbandry

Experiments were performed using C57BL/6J female mice purchased fromJackson Laboratories (Bar Harbor, Me.) and housed in ventilated sterilecages. All animals were maintained in a specific-pathogen-free facility.Animals were acclimated to the vivarium for at least 3 days prior tostudy (i.e., commencing antibiotic courses). For experiments involvingC. difficile infection, mice were administered 10-10⁴ C. difficile VPI10463 spores in 200 μl PBS by oral gavage. Experiments were performed incompliance with institutional guidelines and approved by theinstitution's Institutional Animal Care and Use Committee. Sterile foodand drinking water were provided to the animals

Live Biotherapeutic Product (LBP) Preparation

Individual bacterial strains were isolated from fecal material obtainedfrom healthy donors. The individual strains were struck out from 15%glycerol freezer stocks onto EG (Eggerth Gagnon) agar plates containing5% horse blood in an anaerobic chamber and incubated for 24-48 hours at37° C. Colonies were inoculated into pre-reduced liquid Peptone YeastGlucose (PYG) media and grown for 24-48 hours until dense (static in theanaerobic chamber). Optical density (OD₆₀₀) of the cultures was assessedand live biotherapeutic product (LBP) cocktails were prepared inside ananaerobic chamber adjusting inputs based upon OD₆₀₀ for equal CFU ratiococktails in PBS (sterile, pre-treated).

C. difficile Colony Forming Unit (CFU) Determination

Fecal pellets were collected, transported to an anaerobic chamber (<2hours), and manually homogenized in 500 μL of pre-reduced PBS using apipette tip and through repeated pipetting. Serial dilutions of fecalhomogenates were prepared in pre-reduced PBS, 100 μL of which was spreadonto cycloserine-cefoxitin-fructose agar with sodium taurocholate(TCCFA) plates, and incubated anaerobically at 37° C. C. difficile CFUswere enumerated at 48 hours.

Murine Susceptibility to C. difficile Infection

Groups of mice were evaluated for susceptibility to C. difficile usingthree antibiotic regimen protocols: (1) an antibiotic cocktail, (2)clindamycin administration, or (3) cefoperazone administration (FIGS. 2and 3). The antibiotic cocktail consisted of kanamycin (0.4 mg/ml),gentamicin (0.035 mg/ml), colistin (0.056 mg/ml), metronidazole (0.215mg/ml), vancomycin (0.045 mg/ml) in the drinking water from day −10 today −3, followed by a single intraperitoneal clindamycin injection (200μg/mouse). The clindamycin administration involved a singleintraperitoneal injection of clindamycin (200 μg/mouse) on day −1. Thenotation of days is relative to day 0, the day of C. difficileinfection.

Mice were treated with the indicated antibiotic regimen as describedabove and then infected with either 10 or 10⁴ C. difficile spores byoral gavage on day 0 (FIGS. 2 and 3). An additional experimental arm wasadded to the antibiotic treatment model in which mice were treated withvancomycin after C. difficile infection (FIG. 4J; black triangles).

Mice were monitored daily following infection for mortality/survival(FIGS. 4A-4D) and weight (FIGS. 4E-4H). Fecal pellets were alsocollected daily and used for C. difficile CFU enumeration, presented asCFU/gram feces (FIGS. 4I-4L).

The groups of mice that received cefoperazone treatment had asignificant change in weight (FIG. 4H) and substantial C. difficilebacterial load in the fecal pellets (FIG. 4L), even followingadministration with 10 C. difficile spores. These results indicated thatthe cefoperazone pre-treatment regimen provided a good model for C.difficile infection and for evaluating protection and/or treatment of C.difficile infection. In the absence of antibiotic treatment prior toinfection, C. difficile infection was not established (FIG. 4I) and allmice survived (FIG. 4A) without significant change in body weight (FIG.4E).

Example 2: Live Biotherapeutic Product (LBP) Preparations ProtectAgainst C. difficile Infection

The following LBP compositions were evaluated for their capacity toprotect and/or treat C. difficile infection:

Composition A,

Composition B,

Composition C,

Composition D,

Composition E (See e.g., Narushima et al., Gut Microbes (2014) 5(3)333-339), and

Composition I: a mixture of Clostridium scindens, Pseudoflavonifractorcapillosus and Blautia hansenii (FIG. 5).

In general, LBP cocktails were mixed in PYG media, and each mouse wasadministered a dose by oral gavage in 250 μL pre-reduced PBS(media-free). For composition E, bacteria were mixed in equal volumes(not equal ratios/CFUs) and administered in a 250 μL dose. Each LBP ofCompositions A-D contained 10⁸ CFUs total in a 250 μL dose, comprised of10⁷ CFU of each of the bacterial strains (FIG. 1), for a total of 10⁸CFU administered to each animal. Composition I contained a total of 10⁶CFUs in a 250 μL dose (approximately 333,000 of each of the 3 bacteriamixed).

Groups of mice were subjected to cefoperazone treatment, as described inExample 1, and were administered the indicated composition by oralgavage 2 days after the cessation of cefoperazone treatment. Twenty-fourhours later, the mice were subjected to infection with 10⁴ C. difficilespores (FIG. 5). Mice evaluated for survival/mortality (FIG. 6), weight(FIGS. 7A-7I, and C. difficile CFUs (FIGS. 8A-8C). The results show thatadministration of Composition B prior to C. difficile infection is aneffective protection and/or treatment against C. difficile infection.

Example 3: Composition B Protects Against and/or Treats C. difficileInfection

Groups of 10-12 week old mice were used in the C. difficile mouse model(FIG. 9). Mice were subjected to cefoperazone treatment as described inExample 1. One group of mice was then administered Composition B (10⁸CFU per mouse) administered by oral gavage, as described in Examples 1and 2, 2 days after the cessation of cefoperazone treatment. The othergroup of mice did not receive a live biotherapeutic product aftercefoperazone treatment (control). Twenty-four hours later, the mice weresubjected to C. difficile infection (10⁴ C. difficile spores) and thenevaluated for survival/mortality (FIG. 10), weight (FIG. 11), and C.difficile burden (CFUs per gram feces; FIG. 12). These results confirmthe results of Example 2 that demonstrate treatment with Composition Bprior to C. difficile infection is an effective protection and/ortreatment against C. difficile infection.

Example 4: LBP Composition F Protects Against and/or Treat C. difficileInfection

FIG. 13 shows the strains of live biotherapeutic product (LBP)Composition F. The genus-species classification indicates the closestspecies based on the sequence of the isolated strain. FIG. 14 shows theclassification by Clostridium cluster of the strains in Composition F.

Groups of mice were administered cefoperazone, as described in theExamples above, then administered LBPs or fecal matter transplant (FMT)from mice or human (FIG. 15). Composition B was administered to theindicated groups on day −1; days −2 and −1; or on days −2, −1, 1, 2, and3, relative to infection with 10⁴ C. difficile spores. Composition F wasadministered to the indicated groups on day −1 or on days −2, −1, 1, 2,and 3, relative to administration of C. difficile spores. Additionalgroups received FMT from mice or from humans (200 μL of a 10% fecalsample s per mouse). Mice were then evaluated for survival/mortality(FIG. 16), weight (FIGS. 17A-17H), and C. difficile burden (CFU/gramfeces) on days 1, 3, 8 and 17 after infection (FIGS. 18A and 18B). Thedata demonstrate that Composition B, Composition F, and FMT protectagainst and/or treat C. difficile infection.

Example 5: LBP Compositions Protect Against and/or Treat C. difficileInfection

FIG. 19 shows the strains of LBP Composition G. The genus-speciesnotation indicates the closest species based on the sequence of theisolated strain. Composition G includes a subset of the strains ofComposition F. Groups of mice were administered cefoperazone, asdescribed in the Examples above, then administered the LBP:

Composition B;

Composition B-1 (Composition B with Bacteroides added);

Composition B-2 (Composition B from which Flavonifractor plautii wasremoved and Bacteroides added);

Composition F;

Composition G;

Human fecal samples subjected to ethanol treatment;

Composition B subjected to ethanol treatment;

Composition B that had been frozen; or

Composition J: Clostridium innocuum, Clostridium bolteae and Clostridiumsymbiosum subjected to ethanol treatment; (See also FIG. 20).

The Bacteroides strain used in Composition B-1 and B-2 was Bacteroidesovatus (strain identifier 211-B; SEQ ID NO: 83).

Mice were challenged with C. difficile VPI 10463 spores (10⁴) andmonitored daily (Day 0 to Day 7 post C. difficile infection) forsurvival/mortality (FIGS. 21 and 23) and change in weight (FIGS. 22A-22Jand 24). These data show that the compositions protect against and/ortreat C. difficile infection.

Example 6: LBP Compositions Protect Against and/or Treat C. difficileInfection

Groups of mice were subjected to cefoperazone treatment, as describedabove, then administered human fecal matter transplant, Composition B,Composition B+4 spores, or Composition H (FIG. 25). “Composition B+4spores” refers to Composition B plus the following four strains in sporeform: Clostridium bolteae, Anaerotruncus colihominis, Clostridiumsymbiosum and Clostridium innocuum. Composition H contains the followingsix strains in spore form: Clostridium bolteae, Anaerotruncuscolihominis, Clostridium symbiosum, Clostridium innocuum, Clostridiumdisporicum and Erysipelatoclostridium ramosum (FIG. 26).

Mice were then challenged with C. difficile infection with 10⁴ C.difficile VPI 10463 spores and monitored for survival/mortality (FIGS.27A and 28A), weight (FIGS. 27B and 28B). Mice that lost more than 20%body weight relative to baseline were included in mortality numbers insurvival curves. The C. difficile burden was assessed by CFU in fecalpellets on days 1, 4 and 19 after infection (FIGS. 29A-29C).

These data indicate that Composition B as well as other compositions canimprove survival in the cefoperazone-induced C. difficile mouse modeland protect against and/or treat C. difficile infection.

Example 7: C. difficile Toxin Experiment

Vero cells, epithelial cells derived from African Green Monkey kidneyepithelium, are sensitive to a variety of bacterial toxins, including C.difficile Toxin B. Exposure of cells to C. difficile Toxin B results ininhibition of the function of Rho, Rac, and Cdc42 leading to a declinein F-actin, a change in cell morphology (e.g., cell rounding), andeventually apoptosis.

To determine whether administration of bacterial compositions describedherein has an effect on the production or activity of C. difficile ToxinB, a cellular assay was performed. Briefly, groups of mice were treatedwith cefoperazone, as described above, and administered human fecalmatter transplant (FMT) (“4-3”); Composition B (“5-3”); Composition Bplus four strains in spore form: Clostridium bolteae, Anaerotruncuscolihominis, Clostridium symbiosum and Clostridium innocuum (“7-4”), orno treatment. Each of the groups of mice were then exposed to C.difficile infection with 10⁴ C. difficile spores. The groups of micethat did not receive a treatment after cefoperazone administration andprior to C. difficile infection are referred to as “2-1 (Cdiff)” and“2-4 (Cdiff).” An additional group of mice was not exposed to C.difficile as indicated by “N3 (Healthy)”.

Fecal pellets were collected from each of the groups of mice, weighed,and homogenized in PBS and normalized to a fixed concentration (˜25mg/mL). The samples were centrifuged to prepare a clarified supernatant,which was then diluted in 10-fold serial dilutions to produce a rangefrom 1:10 to 1:10⁻⁶ dilutions of clarified pellet supernatant. Vero cellcultures were exposed to the diluted samples for approximately 18 hours,then visualized by phase contract microscopy to assess morphologicalchanges (i.e., cell rounding) associated with C. difficile toxinexposure. The cells were scored based on the highest concentration ofsupernatant that did not yield a change in morphology (FIG. 30). Thesamples from mice that had been treated with Composition B prior to C.difficile infection had reduced amounts of C. difficile Toxin B, ascompared to samples from control mice that did not receive a treatmentafter cefoperazone administration and prior to C. difficile infection(“2-1 (Cdiff)” and “2-4 (Cdiff)”) as well as compared to samples frommice that received FMT. Notably, the samples from mice that had beentreated with Composition B also had reduced amounts of C. difficileToxin B, as compared to samples from mice that had been treated withComposition B with additional spores.

Example 8: In Vitro Competition Between Compositions B and C. difficile

Composition B was assessed for its ability to suppress Clostridiumdifficile growth by an in vitro mixed culture competition assay. Fromglycerol freezer stocks, individual strains of Composition B, C.difficile (Cdiff), Clostridium bifermentans, and Bacteroidesthetaiotaomicron were struck out onto Eggerth-Gagnon agar plates withhorse blood (EG+HB). Single colonies of each of the strains weresubsequently inoculated into brain heart infusion (BHI) liquid media andallowed to grow in pure culture for 24-48 hours. Turbid cultures weresub-cultured then grown to exponential phase and finally diluted andcombined to prepare a mixed culture with an optical density (OD₆₀₀) of0.1. Exponential phase Cdiff culture was added to the mixed culture at afinal concentration with an OD of 0.1. After the cultures were combinedand incubated for 2-3 hours, samples were collected, serially diluted,and plated on Taurocholate-Cycloserine-Cefoxitin-Fructose Agar (TCCFA)plates to select for Cdiff growth. After 48-72 hours, the colony formingunits (CFUs) of Cdiff in each competition experiment were determined bymanual colony counting.

EG+HB agar plates were prepared according to standard procedures andreduced in an anaerobic environment for at least 6-8 hours prior to use.Liquid BHI medium was obtained from BD Biosciences (Catalog #211059, SanJose, Calif.), prepared according to the manufacturer's instructions,and reduced in an anaerobic environment for at least 18-24 hours priorto use. TCCFA plates were prepared according to standard procedures andreduced in an anaerobic environment for at least 6-8 hours prior to use.Clostridium difficile strain used in the experiments: American TypeCulture Collection (ATCC) 43255.

TABLE 4 Composition B strains Composition B VE202-7 VE202-13 VE202-14VE202-16 Strain #16 Strain #170 Strain #189 Strain #211

Strains were struck out onto EG+HB agar plates from frozen glycerolstocks inside an anaerobic chamber for 48-72 hours. Single colonies wereinoculated into 10 mL of BHI media and grown 24-48 hours at 37° C. inthe anaerobic chamber. Turbid cultures were then diluted to an OD of 0.1and grown for 2-3 hours at 37° C. in the anaerobic chamber. Exponentialphase cultures were diluted and combined at equivalent ODs. For thecompetition assay, each of the strains of Combination B (Table 4) werecombined in equal parts, based on OD₆₀₀, to reach a final consortiumOD₆₀₀ of 0.1. C. bifermentans and B. thetaiotaomicron were setup tocompete with Cdiff individually at an OD of 0.1. The OD₆₀₀ for Cdiff ineach of the mixed culture competition experiments was 0.1. Aftercombination, the cultures were incubated for 2-3 hours at 37° C. in theanaerobic chamber, then prepared for enumerations on Cdiff selectiveplates.

TCCFA plates are selective for Cdiff growth, and none of the CombinationB strains, nor either of the control strains (C. bifermentans and B.thetaiotaomicron), grow on these plates. Inside an anaerobic chamber, a100 μL sample of each competition culture was collected and seriallydiluted 1:10 to reach a final dilution of 1×10⁻⁶. Plates for CFUenumeration were prepared by spreading 100 μL of each of the 1×10through 1×10⁻⁶ dilutions on TCCFA plates using sterile spreading loops.CFU plates were incubated for 48-72 hours at 37° C. in the anaerobicchamber. CFU enumeration was completed by manually counting colonies.

To determine the effect of competition, the ratio of CFUs determined forthe competition samples and Cdiff alone was calculated and expressed asa percentage. Inhibition of Cdiff growth by the Composition B cocktailwas compared to the responses of B. thetaiotaomicron (negative control)and C. bifermentans (positive control). The results are shown in Table 5and FIG. 31.

TABLE 5 Summary Results for In Vitro Competition Ex- No CompetitionCompetition Competition periment Competing with B. with C. with NumberStrain(s) thetaiotaomicron bifermentans Composition B n = 1 100 33.8 n =2 100 9.90 0.1 0.5 n = 3 100 115 39.5 33.1 n = 4 100 41.3 0.7 0.7 n = 5100 105 14.1 20.9 n = 6 100 57.4 4.1 1.6 Mean 100 65.6 11.7 15.1 Std.Dev. 0 43.8 16.5 16.2 Total N 6 5 5 6

Data is expressed as Cdiff CFU as a percentage of control. Each n isrepresentative of a single biological replicate, independent of othermeasurements.

In in vitro competition, Composition B inhibited Cdiff growth to15.1±16.2% of control (absence of competing strain(s)). This result isconsistent with the inhibition observed by the positive control, C.bifermentans, of 11.7±16.5% of control. B. thetaiotaomicron, a negativecontrol, yielded a negligible effect on Cdiff growth at 65.6±43.8% ofcontrol. Given the variability inherent in the assessment of CFU,inhibition of growth to <25% of control is considered to be significantinhibition and both the positive control and Composition B cocktail meetthis threshold of activity. The Composition B consortium attenuatedCdiff growth in vitro comparable to the direct competition observed byC. bifermentans. Direct competition with B. thetaiotaomicron did notsignificantly inhibit Cdiff growth.

Example 9: Determination of In Vitro Short-Chain Fatty Acid Production

Each strain of Composition B was assessed for individual short-chainfatty acid (SCFA) production in vitro. Composition B strains were grownin pure cultures inside an anaerobic chamber. Spent supernatant fromliquid media cultures was harvested by centrifugation, filtersterilized, and then stored at <−70° C. Frozen clarified supernatantspecimens were analyzed for short-chain fatty acids (SCFAs).

EG+HB agar plates (Eggerth-Gagnon agar plates with horse blood) wereprepared according to standard methods and reduced in an anaerobicenvironment for at least 6-8 hours prior to use. Liquid PYG medium(pre-formulated, pre-reduced) was obtained from Anaerobe Systems(Catalog#AS-822; Morgan Hill, Calif.).

Strains were struck out onto EG+HB agar plates from frozen 15% glycerolstocks inside an anaerobic chamber for 48-72 hours. Single colonies wereinoculated into 7 mL PYG media and grown 24-48 hours at 37° C. in theanaerobic chamber. Unless otherwise noted, when the optical density (OD)was >0.2, samples were collected for CFU enumeration and filtration.Inside an anaerobic chamber, a 100 μL sample of turbid culture wascollected and serially diluted 1:10 to reach a final dilution of 1×10⁻⁶.Plates for CFU enumeration were prepared by spreading 100 μL/dilutionfor the 1×10⁻⁴ through 1×10⁻⁶ dilutions on EG+HB agar plates usingsterile glass beads. CFU plates were incubated for 48-72 hours in theanaerobic chamber. CFU enumeration was completed using the EasyCount 2(bioMérieux SA, Marcy-l′Étoile, France). Immediately after samples ofturbid cultures were collected for CFU enumeration, the remaining turbidcultures were centrifuged at approximately 1000 RCF for 10 minutes topellet cellular debris. The clarified supernatants were transferred to a0.2 μm plate filter and vacuum filtered to remove any remainingparticulates prior to bioanalysis. In the event of blockage in thefilter plate, clarified supernatants were manually filtered using 0.2 μmsyringe filters. Filtered supernatants were aliquoted and stored at<−70° C. prior to bioanalysis of SCFAs.

To facilitate easier comparisons between samples, raw SCFA data (μg/mL)was normalized by the login of corresponding determined/estimated CFUfor the culture. The results are depicted in Table 6 and Table 7 below.

TABLE 6 Enumerated CFUs for Composition B Strains Enumerated CFU SampleID OD600 (CFU/mL) VE202-7 >2 6.11E+08 VE202-13 0.8 4.00E+08 VE202-14 >21.60E+09 VE202-16 1.92 1.28E+09 #16 1.97 1.69E+08 #170 1.8 1.08E+08 #1891.03 1.74E+09 #211 0.35 3.71E+08

TABLE 7 SFCAs produced by individual Composition B strains Normalized(μg/Log(CFU/mL)*mL) 2- Sample Iso- Methyl- Iso- ID Acetate Propionatebutyrate Butyrate butyrate valerate Valerate Hexanoate VE202-7 123.70.077 0.102 0.208 0.015 0.056 BLOQ 0.031 VE202-13 30.1 0.545 0.11634.452 0.288 0.188 0.097 0.034 VE202-14 110.5 0.054 0.022 0.248 0.0110.014 BLOQ 0.009 VE202-16 313.2 0.000 0.000 0.280 0.004 0.000 BLOQ 0.009#16 104.0 0.005 0.000 50.988 0.014 0.033 BLOQ 0.009 # 170 87.1 0.0550.025 0.215 0.011 0.039 BLOQ 0.016 # 189 0.0 BLOQ 0.000 35.751 0.0050.019 0.359 0.587 # 211 57.6 5.289 0.000 78.227 0.028 0.050 0.053 0.095

Seven strains of Composition B were found to produce significantquantities (>1 μg/Log(CFU/mL)*mL) of the 2-carbon SCFA, acetate. Onestrain, (#211), produced substantial quantities of the 3-carbon SCFA,propionate. Four strains of Composition B produced substantialquantities of the 4-carbon SCFA, butyrate. Trace quantities (<1μg/Log(CFU/mL)*mL) of other SCFAs were also produced by the CompositionB strains.

Example 10: Composition B Induces Regulatory T Cells (Tress)

Each of the bacterial strains of Composition B were grown to log phase,combined to a total dose of −10⁸ cfu per mouse. Germ-free mice wereinoculated with Composition B or a negative control by oral gavage andsacrificed following four weeks of colonization. Lamina proprialeukocytes were isolated from colonic tissue of individual mice bystandard procedures and assessed by flow cytometry. The regulatory Tcell content was evaluated as the percentage of Foxp3-positive cellsamong CD4+ T cells.

As shown in FIG. 32, mice that were inoculated with Composition B werefound to have significantly more regulatory T cells as compared to micethat were inoculated with the control.

What is claimed is:
 1. A pharmaceutical composition comprising apurified bacterial mixture consisting of bacterial strains comprising16S rDNA sequences of at least 97% sequence identity to SEQ ID NO:10,SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19,SEQ ID NO:20, and SEQ ID NO:21; wherein the bacterial strains are inpowdered form.
 2. The pharmaceutical composition of claim 1, wherein oneor more of the bacterial strains are spore-formers.
 3. Thepharmaceutical composition of claim 1, wherein the bacterial strainsoriginate from more than one human donor.
 4. The pharmaceuticalcomposition of claim 1, further comprising a pharmaceutically acceptableexcipient.
 5. The pharmaceutical composition of claim 1, wherein thepharmaceutical composition is formulated for oral administration.
 6. Thepharmaceutical composition of claim 1, wherein the pharmaceuticalcomposition is in the form of a capsule.
 7. The pharmaceuticalcomposition of claim 1, wherein the pharmaceutical composition isformulated for delivery to the colon.
 8. The pharmaceutical compositionof claim 1, wherein the pharmaceutical composition further comprises oneor more enteric polymers.
 9. A pharmaceutical composition comprising apurified bacterial mixture consisting of bacterial strains comprising16S rDNA sequences of at least 97% sequence identity to SEQ ID NO:10,SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19,SEQ ID NO:20, and SEQ ID NO:21; and one or more enteric polymers. 10.The pharmaceutical composition of claim 9, wherein one or more of thebacterial strains are spore-formers.
 11. The pharmaceutical compositionof claim 9, wherein the bacterial strains originate from more than onehuman donor.
 12. The pharmaceutical composition of claim 9, furthercomprising a pharmaceutically acceptable excipient.
 13. Thepharmaceutical composition of claim 9, wherein the pharmaceuticalcomposition is formulated for oral administration.
 14. Thepharmaceutical composition of claim 9, wherein the pharmaceuticalcomposition is in the form of a capsule.
 15. The pharmaceuticalcomposition of claim 9, wherein the pharmaceutical composition isformulated for delivery to the colon.